Proteintech

Myelin basic protein Polyclonal Antibody

Myelin basic protein Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 10

Myelin basic protein Antibody (10458-1-AP) in IHC (P)

Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using (Product # 10458-1-AP) (Myelin basic protein antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Myelin basic protein Antibody in Western Blot (WB)

Product Image Gallery

Product Details

10458-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:6,000

View 33 publications 33 publications

Immunohistochemistry (IHC)

View 17 publications 17 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

View 39 publications 39 publications

Flow Cytometry (Flow)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Myelin basic protein Fusion Protein Ag0713 (1-171 aa encoded by BC008749)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

10458-1-AP is capable of recognizing multiple isoforms of MBP.

Immunogen sequence: MASQKRPSQ RHGSKYLATA STMDHARHGF LPRHRDTGIL DSIGRFFGGD RGAPKRGSGK DSHHPARTAH YGSLPQKSHG RTQDENPVVH FFKNIVTPRT PPPSQGKGRG LSLSRFSWGA EGQRPGFGYG GRASDYKSAH KGFKGVDAQG TLSKIFKLGG RDSRSGSPMA RR (1-171 aa encoded by BC008749)

Target Information

The protein encoded by the classic MBP gene is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the nervous system. However, MBP-related transcripts are also present in the bone marrow and the immune system. These mRNAs arise from the long MBP gene (otherwise called "Golli-MBP") that contains 3 additional exons located upstream of the classic MBP exons. Alternative splicing from the Golli and the MBP transcription start sites gives rise to 2 sets of MBP-related transcripts and gene products. The Golli mRNAs contain 3 exons unique to Golli-MBP, spliced in-frame to 1 or more MBP exons. They encode hybrid proteins that have N-terminal Golli aa sequence linked to MBP aa sequence. The second family of transcripts contain only MBP exons and produce the well characterized myelin basic proteins. This complex gene structure is conserved among species suggesting that the MBP transcription unit is an integral part of the Golli transcription unit and that this arrangement is important for the function and/or regulation of these genes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Mon Oct 01 00:00:00 EDT 2018

Mesenchymal Stem Cell-Derived Exosomes Reduce A1 Astrocytes via Downregulation of Phosphorylated NFκB P65 Subunit in Spinal Cord Injury.

Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology

Wang L,Pei S,Han L,Guo B,Li Y,Duan R,Yao Y,Xue B,Chen X,Jia Y

Fri Nov 09 00:00:00 EST 2018

Mesenchymal Stem Cell-Derived Exosomes Reduce A1 Astrocytes via Downregulation of Phosphorylated NFκB P65 Subunit in Spinal Cord Injury.

Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology

Wang L,Pei S,Han L,Guo B,Li Y,Duan R,Yao Y,Xue B,Chen X,Jia Y

Fri Nov 09 00:00:00 EST 2018

Sigma-1 receptor activation alleviates blood-brain barrier dysfunction in vascular dementia mice.

Experimental neurology

Liu DY,Chi TY,Ji XF,Liu P,Qi XX,Zhu L,Wang ZQ,Li L,Chen L,Zou LB

Mon Oct 01 00:00:00 EDT 2018

ST8SIA2 promotes oligodendrocyte differentiation and the integrity of myelin and axons.

Glia