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Myeloperoxidase Antibody (100-4181) in WB
Anti-Myeloperoxidase [Human Leukocytes] detects multiple MPO subunits and chain combinations by western blot. Polyclonal rabbit-anti-Myeloperoxidase was used at a 1:5000 dilution to detect 1.0 µg of human myeloperoxidase. This antibody detects a multiple bands corresponding to 53 kDa and 15 kDa polypeptides and chain combinations forming 68 kDa and 106 kDa proteins. The staining of the 68 kDa band is so intense that is over saturates the signal detection. A 4-20% gradient gel was used to separate the protein by SDS-PAGE. The protein was tr... View More
Product Details
100-4181
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Myeloperoxidase [Human Leukocytes]
Conjugate
Form
Lyophilized
Concentration
75 mg/mL
Storage buffer
0.02M potassium phosphate/whole serum, pH 7.2, with 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
Product Specific Information
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, purified and partially purified Myeloperoxidase [Human Leukocytes]. Cross reactivity against Myeloperoxidase from other tissues and species may occur but have not been specifically determined.
Target Information
Myeloperoxidase (MPO) is a heme protein that is synthesized during myeloid differentiation and is a major component of neutrophil azurophilic granules. It is produced as a single chain precursor and subsequently cleaved into a light and heavy chain. The mature MPO is a tetramer composed of 2 light chains and 2 heavy chains. This enzyme is responsible for producing hypohalous acids that are central to the microbicidal activity of neutrophils. Diseases that are associated with MPO include Myeloperoxidase Deficiency and Alzheimer's Disease, Familial, 1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: MPO; Myeloperoxidase
Gene Aliases: MPO
UniProt ID: (Human) P05164
Entrez Gene ID: (Human) 4353
response to yeast
hypochlorous acid biosynthetic process
respiratory burst involved in defense response
defense response
response to oxidative stress
aging
response to mechanical stimulus
removal of superoxide radicals
response to food
response to lipopolysaccharide
low-density lipoprotein particle remodeling
hydrogen peroxide catabolic process
negative regulation of apoptotic process
negative regulation of growth of symbiont in host
defense response to fungus
oxidation-reduction process
response to gold nanoparticle
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