Invitrogen

N-cadherin Monoclonal Antibody (8C11)

Advanced Verification

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

2 Published Figures

7 References

View all (97) N-cadherin antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

N-cadherin Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 12

N-cadherin Antibody (MA1-2002) in ICC/IF

Immunofluorescent analysis of N-Cadherin in SHSY5Y cells (human neuroblast). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed without (left panel) or with (right panel) an N-Cadherin monoclonal antibody (Product # MA1-2002) at a dilution of 1... View More

N-cadherin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

N-cadherin Antibody in Western Blot (WB)

N-cadherin Antibody in Immunoprecipitation (IP)

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Product Details

MA1-2002

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 5 publications 5 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20

Immunocytochemistry (ICC/IF)

1:50-1:200

Immunoprecipitation (IP)

5 µg

Product Specifications

Species Reactivity

Hamster, Human, Mouse, Rabbit

Published species

Avian, Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

8C11

Immunogen

Bacterial fusion protein corresponding to the extracellular domain of N-cadherin.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_326076

Product Specific Information

MA1-2002 detects N-Cadherin from human neuronal samples, including SHSY5Y cells. MA1-2002 also detects N-Cadherin in rabbit and hamster brain lysates. MA1-2002 does not detect N-Cadherin in lysates from mouse, rat, pig and cow brain. This antibody is not suitable for mouse C2C12 cell lysates in Western blot analysis.

MA1-2002 has been successfully used in Western blot, Immunoprecipitation, IHC (P) and Immunofluorescence applications. By Western blot, this antibody detects N-Cadherin at 130 kDa. This antibody is not suitable for frozen tissues in immunohistochemistry applications.

The MA1-2002 immunogen is a bacterial fusion protein to the extracellular domain of N-cadherin (a domain between EC3 and EC4, amino acid residues 92-593 from human N-cadherin).

Target Information

N-cadherin is a 140 kDa protein belonging to a family of transmembrane molecules that mediate calcium-dependent intercellular adhesion. Cadherins are involved in controlling morphogenetic movements during development and regulate cell surface adhesion through homotypic adhesion with the same cadherin species. N-cadherin's function is dependent on its association with the actin-cytoskeleton and is mediated through interactions between the C-terminal region of N-cadherin and the cytoplasmic catenin proteins. The stability of this association is regulated by phosphorylation and dephosphorylation of beta-catenin. Further, N-cadherin is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Transforming Growth Factor Beta and Epithelial to Mesenchymal Transition Alter Homologous Recombination Repair Gene Expression and Sensitize BRCA Wild-Type Ovarian Cancer Cells to Olaparib.

Cancers

Roberts CM,Rojas-Alexandre M,Hanna RE,Lin ZP,Ratner ES

MA1-2002 was used in Western Blotting to find that TGFβ and EMT resulted in the downregulation of genes responsible for homologous recombination (HR) and sensitized cells to olaparib.

Tue Aug 01 00:00:00 EDT 2023

Exosome-mediated mRNA delivery in vivo is safe and can be used to induce SARS-CoV-2 immunity.

The Journal of biological chemistry

Tsai SJ,Atai NA,Cacciottolo M,Nice J,Salehi A,Guo C,Sedgwick A,Kanagavelu S,Gould SJ

MA1-2002 was used in Western Blot to describe an alternative approach using exosomes, the only biologically normal nanovesicle.

Mon Nov 01 00:00:00 EDT 2021

Exosome-mediated mRNA delivery in vivo is safe and can be used to induce SARS-CoV-2 immunity.

The Journal of biological chemistry

Tsai SJ,Atai NA,Cacciottolo M,Nice J,Salehi A,Guo C,Sedgwick A,Kanagavelu S,Gould SJ

MA1-2002 was used in Western Blot to describe an alternative approach using exosomes, the only biologically normal nanovesicle.

Mon Nov 01 00:00:00 EDT 2021

The Motility and Mesenchymal Features of Breast Cancer Cells Correlate with the Levels and Intracellular Localization of Transglutaminase Type 2.

Cells

Bianchi N,Brugnoli F,Grassilli S,Bourgeois K,Keillor JW,Bergamini CM,Aguiari G,Volinia S,Bertagnolo V

MA1-2002 was used in Western Blot to investigate motility in breast cancer cell lines in association with the expression of Transglutaminase type 2 as well as upon the administration of Doxorubicin, an active cytotoxic agent that is employed in chemotherapy.

Sat Nov 06 00:00:00 EDT 2021

A mechanically active heterotypic E-cadherin/N-cadherin adhesion enables fibroblasts to drive cancer cell invasion.

Nature cell biology

Labernadie A,Kato T,Brugués A,Serra-Picamal X,Derzsi S,Arwert E,Weston A,González-Tarragó V,Elosegui-Artola A,Albertazzi L,Alcaraz J,Roca-Cusachs P,Sahai E,Trepat X

MA1-2002 was used in Immunohistochemistry to investigate the interactions between cancer-associated fibroblasts and cancer cells which drive cooperative tumour invasion.

Wed Mar 01 00:00:00 EST 2017

Control of cell-cell forces and collective cell dynamics by the intercellular adhesome.

Nature cell biology

Bazellières E,Conte V,Elosegui-Artola A,Serra-Picamal X,Bintanel-Morcillo M,Roca-Cusachs P,Muñoz JJ,Sales-Pardo M,Guimerà R,Trepat X

MA1-2002 was used in immunohistochemistry to elucidate the contributions of cell-cell adhesion proteins to intercellular force and epithelial tissue dynamics.

Wed Apr 01 00:00:00 EDT 2015

Distinct PTPmu-associated signaling molecules differentially regulate neurite outgrowth on E-, N-, and R-cadherin.

Molecular and cellular neurosciences

Oblander SA,Brady-Kalnay SM

MA1-2002 was used in western blot to investigate the effects of PTPmu on cadherin-dependent neurite outgrowth

Sat May 01 00:00:00 EDT 2010

N-cadherin-catenin complexes form prior to cleavage of the proregion and transport to the plasma membrane.

The Journal of biological chemistry

Wahl JK,Kim YJ,Cullen JM,Johnson KR,Wheelock MJ

MA1-2002 was used in western blot to investigate N-cadherin processing by proteolysis and the formation of N-cadherin-catenin complexes

Fri May 09 00:00:00 EDT 2003

Bioinformatics

Protein Aliases: cadherin 2, type 1 preproprotein; cadherin 2, type 1, N-cadherin (neuronal); Cadherin-2; Cadherin2; calcium-dependent adhesion protein, neuronal; CD325; CDw325; CDw325 antigen; N-cadherin; N-cadherin 1; Neural cadherin

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Gene Aliases: CD325; CDH2; CDHN; CDw325; I79_014914; N-cadherin; NCAD

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UniProt ID: (Human) P19022, (Mouse) P15116

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Entrez Gene ID: (Human) 1000, (Rabbit) 100344571, (Mouse) 12558, (Chinese hamster) 100689204

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Function(s)

calcium ion binding protein binding beta-catenin binding protein kinase binding protein phosphatase binding protein complex binding alpha-catenin binding gamma-catenin binding nitric-oxide synthase binding enzyme binding metal ion binding cadherin

Process(es)

cell adhesion homophilic cell adhesion via plasma membrane adhesion molecules heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules glial cell differentiation calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules cell migration cerebral cortex development regulation of myelination adherens junction organization regulation of Rho protein signal transduction positive regulation of MAPK cascade cell-cell adhesion mediated by cadherin blood vessel morphogenesis brain morphogenesis homeostasis of number of cells regulation of axonogenesis striated muscle cell differentiation positive regulation of muscle cell differentiation protein heterooligomerization radial glial cell differentiation neuroepithelial cell differentiation regulation of oligodendrocyte progenitor proliferation establishment of protein localization to plasma membrane negative regulation of canonical Wnt signaling pathway neuroligin clustering involved in postsynaptic membrane assembly neuronal stem cell population maintenance regulation of postsynaptic density protein 95 clustering positive regulation of synaptic vesicle clustering synapse assembly regulation of signal transduction single organismal cell-cell adhesion regulation of protein localization

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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N-cadherin Monoclonal Antibody (8C11)

N-cadherin Antibody (MA1-2002) in ICC/IF

Product Details

MA1-2002

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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