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Invitrogen
N-cadherin Monoclonal Antibody (CH-19)
Advanced Verification
This Antibody was verified by Independent antibody to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 22
N-cadherin Antibody (MA1-91128) in ICC/IF
Immunofluorescence analysis of N-cadherin was performed using 90% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with N-cadherin Monoclonal Antibody (CH-19) (Product # MA1-91128) at 1:250 dilution in 0.1% BSA, incubated at 4 de... View More
Product Details
MA1-91128
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Dog,
Chicken,
Cat,
Guinea pig,
Goat,
Hamster,
Human,
Mouse,
Sheep,
Pig,
Rabbit,
Reptile,
Rat
Published species
Horse,
Mouse
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
CH-19
Immunogen
synthetic peptide DYDYLNDWGPRFKKLADMYGGGDD conjugated to KLH, corresponding to amino acids 889-912 of Chicken N-Cadherin (cdh2).
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
Ascites
Contains
15mM sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_1958717
Product Specific Information
Recommended positive controls: MDBK.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
N-cadherin is a 140 kDa protein belonging to a family of transmembrane molecules that mediate calcium-dependent intercellular adhesion. Cadherins are involved in controlling morphogenetic movements during development and regulate cell surface adhesion through homotypic adhesion with the same cadherin species. N-cadherin's function is dependent on its association with the actin-cytoskeleton and is mediated through interactions between the C-terminal region of N-cadherin and the cytoplasmic catenin proteins. The stability of this association is regulated by phosphorylation and dephosphorylation of beta-catenin. Further, N-cadherin is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: cadherin 2 type 1 N-cadherin (neuronal); cadherin 2, type 1 preproprotein; cadherin 2, type 1, N-cadherin (neuronal); Cadherin-2; Cadherin2; calcium-dependent adhesion protein, neuronal; CD325; CDw325; CDw325 antigen; N-cadherin; N-cadherin 1; Neural cadherin; neural calcium-dependent cell adhesion molecule; neural-cadherin; unnamed protein product
Gene Aliases: cadherin-2; CD325; CDH2; CDHN; CDw325; I79_014914; N-cadherin; NCAD
UniProt ID: (Human) P19022, (Chicken) P10288, (Mouse) P15116, (Rat) Q9Z1Y3
calcium ion binding
protein binding
beta-catenin binding
protein kinase binding
protein phosphatase binding
protein complex binding
alpha-catenin binding
gamma-catenin binding
nitric-oxide synthase binding
identical protein binding
cadherin binding
extracellular matrix binding
enzyme binding
metal ion binding
cadherin
cell adhesion
homophilic cell adhesion via plasma membrane adhesion molecules
heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules
glial cell differentiation
calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules
cell migration
cerebral cortex development
regulation of myelination
adherens junction organization
regulation of Rho protein signal transduction
positive regulation of MAPK cascade
cell-cell adhesion mediated by cadherin
blood vessel morphogenesis
brain morphogenesis
homeostasis of number of cells
regulation of axonogenesis
striated muscle cell differentiation
positive regulation of muscle cell differentiation
protein heterooligomerization
radial glial cell differentiation
neuroepithelial cell differentiation
regulation of oligodendrocyte progenitor proliferation
establishment of protein localization to plasma membrane
negative regulation of canonical Wnt signaling pathway
neuroligin clustering involved in postsynaptic membrane assembly
neuronal stem cell population maintenance
regulation of postsynaptic density protein 95 clustering
positive regulation of synaptic vesicle clustering
regulation of signal transduction
cell-cell adhesion
cartilage condensation
neural tube formation
synapse assembly
brain development
negative regulation of cell migration
neural crest cell delamination
regulation of synaptic transmission, glutamatergic
limb development
trigeminal ganglion formation
trigeminal ganglion structural organization
protein localization to plasma membrane
cell-cell adhesion via plasma-membrane adhesion molecules
cell aggregation
single organismal cell-cell adhesion
regulation of protein localization
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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