Invitrogen

N-cadherin Monoclonal Antibody (NCD-2)

N-cadherin Antibody in Immunohistochemistry (IHC)

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N-cadherin Antibody (13-2100) in IHC

Immunofluorescent staining of the developing chick neural tube using N-Cadherin Monoclonal Antibody, Rat (Product # 13-2100).

N-cadherin Antibody in Western Blot (WB)

N-cadherin Antibody in Immunocytochemistry (ICC/IF)

N-cadherin Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

N-cadherin Antibody in Western Blot, Immunohistochemistry (WB, IHC)

N-cadherin Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))

N-cadherin Antibody in Western Blot, Immunohistochemistry (PFA fixed) (WB, IHC (PFA))

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Product Details

13-2100

Applications

Tested Dilution

Publications

Western Blot (WB)

1-10 µg/mL

View 8 publications 8 publications

Immunohistochemistry (IHC)

View 6 publications 6 publications

Immunohistochemistry (Paraffin) (IHC (P))

View 1 publication 1 publication

Immunohistochemistry (PFA fixed) (IHC (PFA))

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

10 µg/mL

Immunocytochemistry (ICC/IF)

View 5 publications 5 publications

Flow Cytometry (Flow)

View 1 publication 1 publication

Neutralization (Neu)

View 1 publication 1 publication

Inhibition Assays (IA)

100 µg/mL

Affinity Purification (AP)

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Chicken, Xenopus

Published species

Avian, Chicken, Hamster, Human, Mouse, Xenopus

Host/Isotype

Rat / IgG2a

Class

Monoclonal

Type

Antibody

Clone

NCD-2

Immunogen

Neuronal tissue of chick embryos.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

2.0 mg/mL

Purification

purified

Storage buffer

PBS, pH 7.4, with 1% BSA

Contains

no preservative

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533007

Product Specific Information

Source: Monoclonal antibody was obtained by fusing the mouse myeloma cell-line P3-X63-Ag8-U1 with spleen cells of Wistar rat after immunization with nervous tissue of chick embryos. The monoclonal antibody was harvested from ascitic fluid of SCID mouse.

Specificity: This antibody specifically reacts with chicken N-cadherin. This antibody inhibits N-cadherin dependent cell-cell contact.

Cross reactivity: This antibody reacts with Xenopus N-cadherin. This antibody does not react with human or mouse N-cadherin.

Storage: 4°C This product does not contain preservative. The stock solution(2.0 mg/mL) should be stored in aliquots at -20°C for 1 year, or should be stored at 4°C for 6 months after adding 0.1% sodium azide. Avoid repeated freeze-thaw cycles.

Reconstitution: Dissolve the lyophilized antibody in 50 µL of distilled water (final concentration: 2.0 mg/mL). This solution can be used as a stock solution. If dilution is necessary for your application, dilute the stock solution with the following Dilution solution just prior to use. When the entire amount of antibody is to be used over a short time period, it may be dissolved directly in 500 µL or more of the Dilution solution. Be sure to store the antibody at a minimum concentration of 2.0 mg/mL. A lower antibody concentration may result in decreased stability. Reconstituted antibody solution should contain 0.1% sodium azide as a preservative when stored at 4°C.

Ca2+ should be contained in the buffers to stabilize cadherin antigen.

Target Information

N-cadherin is a 140 kDa protein belonging to a family of transmembrane molecules that mediate calcium-dependent intercellular adhesion. Cadherins are involved in controlling morphogenetic movements during development and regulate cell surface adhesion through homotypic adhesion with the same cadherin species. N-cadherin's function is dependent on its association with the actin-cytoskeleton and is mediated through interactions between the C-terminal region of N-cadherin and the cytoplasmic catenin proteins. The stability of this association is regulated by phosphorylation and dephosphorylation of beta-catenin. Further, N-cadherin is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Bioinformatics and Experimental Analyses Reveal MAP4K4 as a Potential Marker for Gastric Cancer.

Genes

Zhang J,Cai X,Cui W,Wei Z

Published figure using N-cadherin monoclonal antibody (Product # 13-2100) in Western Blot

Mon Oct 03 00:00:00 EDT 2022

Dbnl and β-catenin promote pro-N-cadherin processing to maintain apico-basal polarity.

The Journal of cell biology

Herrera A,Menendez A,Torroba B,Ochoa A,Pons S

13-2100 was used in Immunocytochemistry, Western Blot, Immunohistochemistry (PFA fixed) to demonstrate that Dbnl and Drebrin-like β-catenin assist in the maturation of pro-N-cadherin, which is critical for AJ formation and for the recruitment AC components like aPKC and, consequently, for the maintenance of apico-basal polarity.

Mon Jun 07 00:00:00 EDT 2021

Dbnl and β-catenin promote pro-N-cadherin processing to maintain apico-basal polarity.

The Journal of cell biology

Herrera A,Menendez A,Torroba B,Ochoa A,Pons S

Mon Jun 07 00:00:00 EDT 2021

Dbnl and β-catenin promote pro-N-cadherin processing to maintain apico-basal polarity.

The Journal of cell biology

Herrera A,Menendez A,Torroba B,Ochoa A,Pons S

Mon Jun 07 00:00:00 EDT 2021

Dbnl and β-catenin promote pro-N-cadherin processing to maintain apico-basal polarity.

The Journal of cell biology

Herrera A,Menendez A,Torroba B,Ochoa A,Pons S

Mon Jun 07 00:00:00 EDT 2021

Dbnl and β-catenin promote pro-N-cadherin processing to maintain apico-basal polarity.

The Journal of cell biology

Herrera A,Menendez A,Torroba B,Ochoa A,Pons S

Mon Jun 07 00:00:00 EDT 2021

Low-Dose Albendazole Inhibits Epithelial-Mesenchymal Transition of Melanoma Cells by Enhancing Phosphorylated GSK-3β/Tyr216 Accumulation.

Journal of oncology

He Z,Lei S,Liang F,Tan L,Zhang W,Xie L,Zheng H,Lu Y

Published figure using N-cadherin monoclonal antibody (Product # 13-2100) in Western Blot

Fri Dec 31 00:00:00 EST 2021

Redistribution of Adhesive Forces through Src/FAK Drives Contact Inhibition of Locomotion in Neural Crest.

Developmental cell

Roycroft A,Szabó A,Bahm I,Daly L,Charras G,Parsons M,Mayor R

13-2100 was used in In vivo experiments to study the exact role of traction through cell-matrix adhesions, and tension through cell-cell adhesions during contact inhibition of locomotion.

Mon Jun 04 00:00:00 EDT 2018

Asymmetric localization of DLC1 defines avian trunk neural crest polarity for directional delamination and migration.

Nature communications

Liu JA,Rao Y,Cheung MPL,Hui MN,Wu MH,Chan LK,Ng IO,Niu B,Cheah KSE,Sharma R,Hodgson L,Cheung M

Published figure using N-cadherin monoclonal antibody (Product # 13-2100) in Immunofluorescence

Mon Oct 30 00:00:00 EDT 2017

Sustained Wnt/β-catenin signalling causes neuroepithelial aberrations through the accumulation of aPKC at the apical pole.

Nature communications

Herrera A,Saade M,Menendez A,Marti E,Pons S

13-2100 was used in immunohistochemistry and western blot to report that beta-catenin controls the cell fate and polarity of neuroblasts by modulating the expression and localization of aPKC.

Thu Jun 19 00:00:00 EDT 2014

Sustained Wnt/β-catenin signalling causes neuroepithelial aberrations through the accumulation of aPKC at the apical pole.

Nature communications

Herrera A,Saade M,Menendez A,Marti E,Pons S

13-2100 was used in immunohistochemistry and western blot to report that beta-catenin controls the cell fate and polarity of neuroblasts by modulating the expression and localization of aPKC.

Thu Jun 19 00:00:00 EDT 2014

Cell surface localization of α3β4 nicotinic acetylcholine receptors is regulated by N-cadherin homotypic binding and actomyosin contractility.

PloS one

Brusés JL

Published figure using N-cadherin monoclonal antibody (Product # 13-2100) in Immunofluorescence

Tue Nov 26 00:00:00 EST 2013

Cell surface localization of α3β4 nicotinic acetylcholine receptors is regulated by N-cadherin homotypic binding and actomyosin contractility.

PloS one

Brusés JL

Published figure using N-cadherin monoclonal antibody (Product # 13-2100) in Immunofluorescence

Tue Nov 26 00:00:00 EST 2013

Cell surface localization of α3β4 nicotinic acetylcholine receptors is regulated by N-cadherin homotypic binding and actomyosin contractility.

PloS one

Brusés JL

Published figure using N-cadherin monoclonal antibody (Product # 13-2100) in Immunofluorescence

Tue Nov 26 00:00:00 EST 2013

Cell surface localization of α3β4 nicotinic acetylcholine receptors is regulated by N-cadherin homotypic binding and actomyosin contractility.

PloS one

Brusés JL

Published figure using N-cadherin monoclonal antibody (Product # 13-2100) in Immunofluorescence

Tue Nov 26 00:00:00 EST 2013

Cell surface localization of α3β4 nicotinic acetylcholine receptors is regulated by N-cadherin homotypic binding and actomyosin contractility.

PloS one

Brusés JL

Published figure using N-cadherin monoclonal antibody (Product # 13-2100) in Immunofluorescence

Tue Nov 26 00:00:00 EST 2013

N-cadherin modulates voltage activated calcium influx via RhoA, p120-catenin, and myosin-actin interaction.

Molecular and cellular neurosciences

Marrs GS,Theisen CS,Brusés JL

Published figure using N-cadherin monoclonal antibody (Product # 13-2100) in Western Blot

Sun Mar 01 00:00:00 EST 2009

N-cadherin modulates voltage activated calcium influx via RhoA, p120-catenin, and myosin-actin interaction.

Molecular and cellular neurosciences

Marrs GS,Theisen CS,Brusés JL

Published figure using N-cadherin monoclonal antibody (Product # 13-2100) in Western Blot

Sun Mar 01 00:00:00 EST 2009

N-cadherin modulates voltage activated calcium influx via RhoA, p120-catenin, and myosin-actin interaction.

Molecular and cellular neurosciences

Marrs GS,Theisen CS,Brusés JL

Published figure using N-cadherin monoclonal antibody (Product # 13-2100) in Western Blot

Sun Mar 01 00:00:00 EST 2009

N-cadherin modulates voltage activated calcium influx via RhoA, p120-catenin, and myosin-actin interaction.

Molecular and cellular neurosciences

Marrs GS,Theisen CS,Brusés JL

Published figure using N-cadherin monoclonal antibody (Product # 13-2100) in Western Blot

Sun Mar 01 00:00:00 EST 2009

N-cadherin modulates voltage activated calcium influx via RhoA, p120-catenin, and myosin-actin interaction.

Molecular and cellular neurosciences

Marrs GS,Theisen CS,Brusés JL

Published figure using N-cadherin monoclonal antibody (Product # 13-2100) in Western Blot

Sun Mar 01 00:00:00 EST 2009

Retinoic acid is a negative physiological regulator of N-cadherin during early avian heart morphogenesis.

Development, growth & differentiation

Romeih M,Cakstina I,Zile MH

13-2100 was used in blocking or activating experiment to assess the role of N-cadherin in retinoic acid-regulated early cardiovascular morphogenesis

Tue Dec 01 00:00:00 EST 2009

Characterization of supporting cell phenotype in the avian inner ear: implications for sensory regeneration.

Hearing research

Warchol ME

13-2100 was used in immunohistochemistry - paraffin section to review molecules that are expressed by supporting cells in the avian ear

Tue May 01 00:00:00 EDT 2007

The differential adhesion hypothesis: a direct evaluation.

Developmental biology

Foty RA,Steinberg MS

13-2100 was used in immunocytochemistry to review the differential adhesion hypothesis

Tue Feb 01 00:00:00 EST 2005

p120 catenin is required for morphogenetic movements involved in the formation of the eyes and the craniofacial skeleton in Xenopus.

Journal of cell science

Ciesiolka M,Delvaeye M,Van Imschoot G,Verschuere V,McCrea P,van Roy F,Vleminckx K

Sun Aug 15 00:00:00 EDT 2004

Alpha5beta1 integrin mediates strong tissue cohesion.

Journal of cell science

Robinson EE,Zazzali KM,Corbett SA,Foty RA

Wed Jan 15 00:00:00 EST 2003

Cadherin-mediated cell adhesion and tissue segregation: qualitative and quantitative determinants.

Developmental biology

Duguay D,Foty RA,Steinberg MS

13-2100 was used in flow cytometry to examine cadherin-subtype-specific binding specificities.

Wed Jan 15 00:00:00 EST 2003

Tissue spreading on implantable substrates is a competitive outcome of cell-cell vs. cell-substratum adhesivity.

Proceedings of the National Academy of Sciences of the United States of America

Ryan PL,Foty RA,Kohn J,Steinberg MS

Tue Apr 10 00:00:00 EDT 2001

The forkhead transcription factor Foxf1 is required for differentiation of extra-embryonic and lateral plate mesoderm.

Development (Cambridge, England)

Mahlapuu M,Ormestad M,Enerbäck S,Carlsson P

Mon Jan 01 00:00:00 EST 2001

Identification of a neural alpha-catenin as a key regulator of cadherin function and multicellular organization.

Cell

Hirano S,Kimoto N,Shimoyama Y,Hirohashi S,Takeichi M

Fri Jul 24 00:00:00 EDT 1992

Identification of a neural alpha-catenin as a key regulator of cadherin function and multicellular organization.

Cell

Hirano S,Kimoto N,Shimoyama Y,Hirohashi S,Takeichi M

Fri Jul 24 00:00:00 EDT 1992

Bioinformatics

Protein Aliases: cadherin 2, type 1, N-cadherin (neuronal); cadherin 2, type 1, N-cadherin (neuronal) L homeolog; cadherin 2, type 1, N-cadherin a (neuronal); Cadherin-2; Cadherin-2A; Cadherin2; CDw325 antigen; N-cadherin; N-cadherin A; N-cadherin-2; Neural cadherin; Neural cadherin A; Neural cadherin-2; neural calcium-dependent cell adhesion molecule; unnamed protein product

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Gene Aliases: cadherin-2; CDH2; cdh2-a; cdh2-b; cdh2a; cdhn-a; N-Cad; XELAEV_18031953mg

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UniProt ID: (Chicken) P10288, (Xenopus) P33147

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Entrez Gene ID: (Chicken) 414745, (Xenopus) 378518

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Function(s)

calcium ion binding protein binding beta-catenin binding protein kinase binding protein phosphatase binding identical protein binding alpha-catenin binding gamma-catenin binding cadherin binding extracellular matrix binding cadherin

Process(es)

cartilage condensation neural tube formation cell adhesion homophilic cell adhesion via plasma membrane adhesion molecules heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules synapse assembly brain development glial cell differentiation calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules cell migration negative regulation of cell migration neural crest cell delamination positive regulation of MAPK cascade cell-cell adhesion mediated by cadherin blood vessel morphogenesis brain morphogenesis homeostasis of number of cells regulation of axonogenesis striated muscle cell differentiation regulation of synaptic transmission, glutamatergic radial glial cell differentiation limb development neuroepithelial cell differentiation trigeminal ganglion formation trigeminal ganglion structural organization regulation of oligodendrocyte progenitor proliferation protein localization to plasma membrane negative regulation of canonical Wnt signaling pathway neuroligin clustering involved in postsynaptic membrane assembly neuronal stem cell population maintenance cell-cell adhesion cell-cell adhesion via plasma-membrane adhesion molecules cell aggregation regulation of postsynaptic density protein 95 clustering positive regulation of synaptic vesicle clustering

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N-cadherin Monoclonal Antibody (NCD-2)

N-cadherin Antibody (13-2100) in IHC

Product Details

13-2100

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Neutralization (Neu)

Inhibition Assays (IA)

Affinity Purification (AP)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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