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Invitrogen
N-cadherin Recombinant Rabbit Monoclonal Antibody (SY02-46)
Advanced Verification
This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 11
N-cadherin Antibody (MA5-32088) in ICC/IF
Immunocytochemical analysis of N-cadherin in RH-35 cells using a N-cadherin Monoclonal antibody (Product # MA5-32088) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Product Details
MA5-32088
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
SY02-46
Immunogen
Synthetic peptide within Human N Cadherin aa 161-210
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 40% Glycerol, 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2800542
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
N-cadherin is a 140 kDa protein belonging to a family of transmembrane molecules that mediate calcium-dependent intercellular adhesion. Cadherins are involved in controlling morphogenetic movements during development and regulate cell surface adhesion through homotypic adhesion with the same cadherin species. N-cadherin's function is dependent on its association with the actin-cytoskeleton and is mediated through interactions between the C-terminal region of N-cadherin and the cytoplasmic catenin proteins. The stability of this association is regulated by phosphorylation and dephosphorylation of beta-catenin. Further, N-cadherin is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: cadherin 2 type 1 N-cadherin (neuronal); cadherin 2, type 1, N-cadherin (neuronal); Cadherin-2; Cadherin2; calcium-dependent adhesion protein, neuronal; CD325; CDw325; CDw325 antigen; N-cadherin; N-cadherin 1; Neural cadherin
Gene Aliases: CD325; CDH2; CDHN; CDw325; N-cadherin; NCAD
UniProt ID: (Human) P19022, (Mouse) P15116, (Rat) Q9Z1Y3
Entrez Gene ID: (Human) 1000, (Mouse) 12558, (Rat) 83501
cell adhesion
homophilic cell adhesion via plasma membrane adhesion molecules
heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules
glial cell differentiation
calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules
cell migration
cerebral cortex development
regulation of myelination
adherens junction organization
regulation of Rho protein signal transduction
positive regulation of MAPK cascade
cell-cell adhesion mediated by cadherin
blood vessel morphogenesis
brain morphogenesis
homeostasis of number of cells
regulation of axonogenesis
striated muscle cell differentiation
positive regulation of muscle cell differentiation
protein heterooligomerization
radial glial cell differentiation
neuroepithelial cell differentiation
regulation of oligodendrocyte progenitor proliferation
establishment of protein localization to plasma membrane
negative regulation of canonical Wnt signaling pathway
neuroligin clustering involved in postsynaptic membrane assembly
neuronal stem cell population maintenance
regulation of postsynaptic density protein 95 clustering
positive regulation of synaptic vesicle clustering
synapse assembly
regulation of signal transduction
single organismal cell-cell adhesion
regulation of protein localization
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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