Proteintech

NAT10 Polyclonal Antibody

NAT10 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 9

NAT10 Antibody (13365-1-AP) in ICC/IF

Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NAT10 antibody (Product # 13365-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

NAT10 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

13365-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:6,000

View 28 publications 28 publications

Immunohistochemistry (IHC)

View 5 publications 5 publications

Immunocytochemistry (ICC/IF)

1:20-1:200

View 8 publications 8 publications

Immunoprecipitation (IP)

0.5-4.0 µg

View 3 publications 3 publications

RNA Immunoprecipitation (RIP)

View 4 publications 4 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

NAT10 Fusion Protein Ag4184 (676-1025 aa encoded by BC035558)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.17 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

The specificity of this antibody has been tested by siRNA.

Immunogen sequence: EAVSLLEEV ITPRKDLPPL LLKLNERPAE RLDYLGVSYG LTPRLLKFWK RAGFVPVYLR QTPNDLTGEH SCIMLKTLTD EDEADQGGWL AAFWKDFRRR FLALLSYQFS TFSPSLALNI IQNRNMGKPA QPALSREELE ALFLPYDLKR LEMYSRNMVD YHLIMDMIPA ISRIYFLNQL GDLALSAAQS ALLLGIGLQH KSVDQLEKEI ELPSGQLMGL FNRIIRKVVK LFNEVQEKAI EEQMVAAKDV VMEPTMKTLS DDLDEAAKEF QEKHKKEVGK LKSMDLSEYI IRGDDEEWNE VLNKAGPNAS IISLKSDKKR KLEAKQEPKQ SKKLKNRETK NKKDMKLKRK K (676-1025 aa encoded by BC035558)

Target Information

IRS4 encodes the insulin receptor substrate 4, a cytoplasmic protein that contains many potential tyrosine and serine/threonine phosphorylation sites. Tyrosine-phosphorylated IRS4 protein has been shown to associate with cytoplasmic signalling molecules that contain SH2 domains. The IRS4 protein is phosphorylated by the insulin receptor tyrosine kinase upon receptor stimulation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Lysine 2-hydroxyisobutyrylation of NAT10 promotes cancer metastasis in an ac4C-dependent manner.

Cell research

Liao L,He Y,Li SJ,Yu XM,Liu ZC,Liang YY,Yang H,Yang J,Zhang GG,Deng CM,Wei X,Zhu YD,Xu TY,Zheng CC,Cheng C,Li A,Li ZG,Liu JB,Li B

Mon May 01 00:00:00 EDT 2023

Lysine 2-hydroxyisobutyrylation of NAT10 promotes cancer metastasis in an ac4C-dependent manner.

Cell research

Liao L,He Y,Li SJ,Yu XM,Liu ZC,Liang YY,Yang H,Yang J,Zhang GG,Deng CM,Wei X,Zhu YD,Xu TY,Zheng CC,Cheng C,Li A,Li ZG,Liu JB,Li B

Mon May 01 00:00:00 EDT 2023

Lysine 2-hydroxyisobutyrylation of NAT10 promotes cancer metastasis in an ac4C-dependent manner.

Cell research

Liao L,He Y,Li SJ,Yu XM,Liu ZC,Liang YY,Yang H,Yang J,Zhang GG,Deng CM,Wei X,Zhu YD,Xu TY,Zheng CC,Cheng C,Li A,Li ZG,Liu JB,Li B

Mon May 01 00:00:00 EDT 2023

Maternal NAT10 orchestrates oocyte meiotic cell-cycle progression and maturation in mice.

Nature communications

Jiang X,Cheng Y,Zhu Y,Xu C,Li Q,Xing X,Li W,Zou J,Meng L,Azhar M,Cao Y,Tong X,Qin W,Zhu X,Bao J

Thu Jun 22 00:00:00 EDT 2023

Activated SIRT1 contributes to DPT-induced glioma cell parthanatos by upregulation of NOX2 and NAT10.

Acta pharmacologica Sinica

Liang SP,Wang XZ,Piao MH,Chen X,Wang ZC,Li C,Wang YB,Lu S,He C,Wang YL,Chi GF,Ge PF

Sun Oct 01 00:00:00 EDT 2023

Hydroxyapatite-Tethered Peptide Hydrogel Promotes Osteogenesis.

Gels (Basel, Switzerland)

Yu H,Song J,Zhang X,Jiang K,Fan H,Li Y,Zhao Y,Liu S,Hao D,Li G

Thu Dec 08 00:00:00 EST 2022

Ginsenoside Rc Promotes Bone Formation in Ovariectomy-Induced Osteoporosis In Vivo and Osteogenic Differentiation In Vitro.

International journal of molecular sciences

Yang N,Zhang X,Li L,Xu T,Li M,Zhao Q,Yu J,Wang J,Liu Z

Tue May 31 00:00:00 EDT 2022

Ginsenoside Rc Promotes Bone Formation in Ovariectomy-Induced Osteoporosis In Vivo and Osteogenic Differentiation In Vitro.

International journal of molecular sciences

Yang N,Zhang X,Li L,Xu T,Li M,Zhao Q,Yu J,Wang J,Liu Z

Tue May 31 00:00:00 EDT 2022

Facile Fabrication of 3D-Printed Porous Ti6Al4V Scaffolds with a Sr-CaP Coating for Bone Regeneration.

ACS omega

Su S,Chen W,Zheng M,Lu G,Tang W,Huang H,Qu D

Tue Mar 15 00:00:00 EDT 2022

Direct epitranscriptomic regulation of mammalian translation initiation through N4-acetylcytidine.

Molecular cell

Arango D,Sturgill D,Yang R,Kanai T,Bauer P,Roy J,Wang Z,Hosogane M,Schiffers S,Oberdoerffer S

Thu Aug 04 00:00:00 EDT 2022

Kinesin motor KIFC1 is required for tubulin acetylation and actin-dependent spindle migration in mouse oocyte meiosis.

Development (Cambridge, England)

Shan MM,Zou YJ,Pan ZN,Zhang HL,Xu Y,Ju JQ,Sun SC

Tue Mar 01 00:00:00 EST 2022

NAT10-mediated N4-acetylcytidine modification is required for meiosis entry and progression in male germ cells.

Nucleic acids research

Chen L,Wang WJ,Liu Q,Wu YK,Wu YW,Jiang Y,Liao XQ,Huang F,Li Y,Shen L,Yu C,Zhang SY,Yan LY,Qiao J,Sha QQ,Fan HY

Fri Oct 28 00:00:00 EDT 2022

NAT10-mediated N4-acetylcytidine modification is required for meiosis entry and progression in male germ cells.

Nucleic acids research

Chen L,Wang WJ,Liu Q,Wu YK,Wu YW,Jiang Y,Liao XQ,Huang F,Li Y,Shen L,Yu C,Zhang SY,Yan LY,Qiao J,Sha QQ,Fan HY

Fri Oct 28 00:00:00 EDT 2022

Elevation of N-acetyltransferase 10 in hippocampal neurons mediates depression- and anxiety-like behaviors.

Brain research bulletin

Guo XF,Wang XH,Fu YL,Meng Q,Huang BY,Yang R,Guo Y,Du YR,Wang X,Gao Y,Song L,Gong M,Wang S,Li YD,Shi HS,Shi Y

Fri Jul 01 00:00:00 EDT 2022

NAT10 Maintains OGA mRNA Stability Through ac4C Modification in Regulating Oocyte Maturation.

Frontiers in endocrinology

Lin J,Xiang Y,Huang J,Zeng H,Zeng Y,Liu J,Wu T,Liang Q,Liang X,Li J,Zhou C

Tue Aug 09 00:00:00 EDT 2022

NAT10 Maintains OGA mRNA Stability Through ac4C Modification in Regulating Oocyte Maturation.

Frontiers in endocrinology

Lin J,Xiang Y,Huang J,Zeng H,Zeng Y,Liu J,Wu T,Liang Q,Liang X,Li J,Zhou C

Tue Aug 09 00:00:00 EDT 2022

NAT10 Maintains OGA mRNA Stability Through ac4C Modification in Regulating Oocyte Maturation.

Frontiers in endocrinology

Lin J,Xiang Y,Huang J,Zeng H,Zeng Y,Liu J,Wu T,Liang Q,Liang X,Li J,Zhou C

Tue Aug 09 00:00:00 EDT 2022

NAT10 Maintains OGA mRNA Stability Through ac4C Modification in Regulating Oocyte Maturation.

Frontiers in endocrinology

Lin J,Xiang Y,Huang J,Zeng H,Zeng Y,Liu J,Wu T,Liang Q,Liang X,Li J,Zhou C

Tue Aug 09 00:00:00 EDT 2022

Mechanical Force Modulates Alveolar Bone Marrow Mesenchymal Cells Characteristics for Bone Remodeling during Orthodontic Tooth Movement through Lactate Production.

Cells

Zhai M,Cui S,Li L,Cheng C,Zhang Z,Liu J,Wei F

Tue Nov 22 00:00:00 EST 2022

NAT10 promotes cell proliferation by acetylating CEP170 mRNA to enhance translation efficiency in multiple myeloma.

Acta pharmaceutica Sinica. B

Wei R,Cui X,Min J,Lin Z,Zhou Y,Guo M,An X,Liu H,Janz S,Gu C,Wang H,Yang Y

Mon Aug 01 00:00:00 EDT 2022

N4-acetylcytidine regulates the replication and pathogenicity of enterovirus 71.

Nucleic acids research

Hao H,Liu W,Miao Y,Ma L,Yu B,Liu L,Yang C,Zhang K,Chen Z,Yang J,Zheng Z,Zhang B,Deng F,Gong P,Yuan J,Hu Z,Guan W

Fri Sep 09 00:00:00 EDT 2022

N4-acetylcytidine regulates the replication and pathogenicity of enterovirus 71.

Nucleic acids research

Hao H,Liu W,Miao Y,Ma L,Yu B,Liu L,Yang C,Zhang K,Chen Z,Yang J,Zheng Z,Zhang B,Deng F,Gong P,Yuan J,Hu Z,Guan W

Fri Sep 09 00:00:00 EDT 2022

N4-acetylcytidine regulates the replication and pathogenicity of enterovirus 71.

Nucleic acids research

Hao H,Liu W,Miao Y,Ma L,Yu B,Liu L,Yang C,Zhang K,Chen Z,Yang J,Zheng Z,Zhang B,Deng F,Gong P,Yuan J,Hu Z,Guan W

Fri Sep 09 00:00:00 EDT 2022

NAT10 acetylates BCL-XL mRNA to promote the proliferation of multiple myeloma cells through PI3K-AKT pathway.

Frontiers in oncology

Zhang Y,Deng Z,Sun S,Xie S,Jiang M,Chen B,Gu C,Yang Y

Sun Nov 05 00:00:00 EDT 2023

Kinesin KIF15 regulates tubulin acetylation and spindle assembly checkpoint in mouse oocyte meiosis.

Cellular and molecular life sciences : CMLS

Zou YJ,Shan MM,Wan X,Liu JC,Zhang KH,Ju JQ,Xing CH,Sun SC

Thu Jul 14 00:00:00 EDT 2022

Dura cells in the etiopathogenesis of Crouzon syndrome: the effects of FGFR2 mutations in the dura cells on the proliferation of osteoblasts through the hippo/YAP mediated transcriptional regulation pathway.

American journal of translational research

Dong XH,Zhang MZ,Lai CZ,Li CC,Du L,Song GD,Zong XL,Jin XL

Thu Nov 18 00:00:00 EST 2021

NAT10-Mediated N4-Acetylcytidine of RNA Contributes to Post-transcriptional Regulation of Mouse Oocyte Maturation in vitro.

Frontiers in cell and developmental biology

Xiang Y,Zhou C,Zeng Y,Guo Q,Huang J,Wu T,Liu J,Liang Q,Zeng H,Liang X

Tue Aug 17 00:00:00 EDT 2021

NAT10-Mediated N4-Acetylcytidine of RNA Contributes to Post-transcriptional Regulation of Mouse Oocyte Maturation in vitro.

Frontiers in cell and developmental biology

Xiang Y,Zhou C,Zeng Y,Guo Q,Huang J,Wu T,Liu J,Liang Q,Zeng H,Liang X

Tue Aug 17 00:00:00 EDT 2021

ac4C acetylation of RUNX2 catalyzed by NAT10 spurs osteogenesis of BMSCs and prevents ovariectomy-induced bone loss.

Molecular therapy. Nucleic acids

Yang W,Li HY,Wu YF,Mi RJ,Liu WZ,Shen X,Lu YX,Jiang YH,Ma MJ,Shen HY

Fri Dec 03 00:00:00 EST 2021

ac4C acetylation of RUNX2 catalyzed by NAT10 spurs osteogenesis of BMSCs and prevents ovariectomy-induced bone loss.

Molecular therapy. Nucleic acids

Yang W,Li HY,Wu YF,Mi RJ,Liu WZ,Shen X,Lu YX,Jiang YH,Ma MJ,Shen HY

Fri Dec 03 00:00:00 EST 2021

ac4C acetylation of RUNX2 catalyzed by NAT10 spurs osteogenesis of BMSCs and prevents ovariectomy-induced bone loss.

Molecular therapy. Nucleic acids

Yang W,Li HY,Wu YF,Mi RJ,Liu WZ,Shen X,Lu YX,Jiang YH,Ma MJ,Shen HY

Fri Dec 03 00:00:00 EST 2021

Multiple Omics Integration Reveals Key Circular RNAs in Hepatocellular Carcinoma.

Frontiers in oncology

Huang ZL,Huang XY,Huang J,Huang XY,Xu YH,Zhou J,Tang ZY

Sat Apr 23 00:00:00 EDT 2022

Single-Cell RNA-Sequencing Reveals the Breadth of Osteoblast Heterogeneity.

JBMR plus

Yoshioka H,Okita S,Nakano M,Minamizaki T,Nubukiyo A,Sotomaru Y,Bonnelye E,Kozai K,Tanimoto K,Aubin JE,Yoshiko Y

Tue Jun 01 00:00:00 EDT 2021

Prognostic and Immunological Role of mRNA ac4C Regulator NAT10 in Pan-Cancer: New Territory for Cancer Research?

Frontiers in oncology

Yang C,Wu T,Zhang J,Liu J,Zhao K,Sun W,Zhou X,Kong X,Shi J

Sat Apr 23 00:00:00 EDT 2022

Knee loading repairs osteoporotic osteoarthritis by relieving abnormal remodeling of subchondral bone via Wnt/β-catenin signaling.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Zheng W,Ding B,Li X,Liu D,Yokota H,Zhang P

Sat Feb 01 00:00:00 EST 2020

Acetylation of Cytidine Residues Boosts HIV-1 Gene Expression by Increasing Viral RNA Stability.

Cell host & microbe

Tsai K,Jaguva Vasudevan AA,Martinez Campos C,Emery A,Swanstrom R,Cullen BR

Wed Aug 12 00:00:00 EDT 2020

Rab23/Kif17 regulate meiotic progression in oocytes by modulating tubulin acetylation and actin dynamics.

Development (Cambridge, England)

Wang HH,Zhang Y,Tang F,Pan MH,Wan X,Li XH,Sun SC

Mon Feb 04 00:00:00 EST 2019

Small molecule inhibitor of TGF-β signaling enables robust osteogenesis of autologous GMSCs to successfully repair minipig severe maxillofacial bone defects.

Stem cell research & therapy

Shi A,Heinayati A,Bao D,Liu H,Ding X,Tong X,Wang L,Wang B,Qin H

Thu Jun 13 00:00:00 EDT 2019

MicroRNA‑223 promotes osteoblast differentiation of MC3T3‑E1 cells by targeting histone deacetylase 2.

International journal of molecular medicine

Chen J,He G,Wang Y,Cai D

Fri Mar 01 00:00:00 EST 2019

CCDC84 Acetylation Oscillation Regulates Centrosome Duplication by Modulating HsSAS-6 Degradation.

Cell reports

Wang T,Zou Y,Huang N,Teng J,Chen J

Tue Nov 12 00:00:00 EST 2019

RAB35 depletion affects spindle formation and actin-based spindle migration in mouse oocyte meiosis.

Molecular human reproduction

Zhang Y,Wan X,Wang HH,Pan MH,Pan ZN,Sun SC

Mon Jul 01 00:00:00 EDT 2019

Acetylation of Cytidine in mRNA Promotes Translation Efficiency.

Cell

Arango D,Sturgill D,Alhusaini N,Dillman AA,Sweet TJ,Hanson G,Hosogane M,Sinclair WR,Nanan KK,Mandler MD,Fox SD,Zengeya TT,Andresson T,Meier JL,Coller J,Oberdoerffer S

Thu Dec 13 00:00:00 EST 2018

Acetylation of Cytidine in mRNA Promotes Translation Efficiency.

Cell

Arango D,Sturgill D,Alhusaini N,Dillman AA,Sweet TJ,Hanson G,Hosogane M,Sinclair WR,Nanan KK,Mandler MD,Fox SD,Zengeya TT,Andresson T,Meier JL,Coller J,Oberdoerffer S

Thu Dec 13 00:00:00 EST 2018

Acetylation of Cytidine in mRNA Promotes Translation Efficiency.

Cell

Arango D,Sturgill D,Alhusaini N,Dillman AA,Sweet TJ,Hanson G,Hosogane M,Sinclair WR,Nanan KK,Mandler MD,Fox SD,Zengeya TT,Andresson T,Meier JL,Coller J,Oberdoerffer S

Thu Dec 13 00:00:00 EST 2018

Acetylation of Cytidine in mRNA Promotes Translation Efficiency.

Cell

Arango D,Sturgill D,Alhusaini N,Dillman AA,Sweet TJ,Hanson G,Hosogane M,Sinclair WR,Nanan KK,Mandler MD,Fox SD,Zengeya TT,Andresson T,Meier JL,Coller J,Oberdoerffer S

Thu Dec 13 00:00:00 EST 2018

Targeting of NAT10 enhances healthspan in a mouse model of human accelerated aging syndrome.

Nature communications

Balmus G,Larrieu D,Barros AC,Collins C,Abrudan M,Demir M,Geisler NJ,Lelliott CJ,White JK,Karp NA,Atkinson J,Kirton A,Jacobsen M,Clift D,Rodriguez R,Adams DJ,Jackson SP

Fri Apr 27 00:00:00 EDT 2018

Targeting of NAT10 enhances healthspan in a mouse model of human accelerated aging syndrome.

Nature communications

Balmus G,Larrieu D,Barros AC,Collins C,Abrudan M,Demir M,Geisler NJ,Lelliott CJ,White JK,Karp NA,Atkinson J,Kirton A,Jacobsen M,Clift D,Rodriguez R,Adams DJ,Jackson SP

Fri Apr 27 00:00:00 EDT 2018

Kif18a regulates Sirt2-mediated tubulin acetylation for spindle organization during mouse oocyte meiosis.

Cell division

Tang F,Pan MH,Wan X,Lu Y,Zhang Y,Sun SC

Wed Mar 30 00:00:00 EDT 2022

Kif18a regulates Sirt2-mediated tubulin acetylation for spindle organization during mouse oocyte meiosis.

Cell division

Tang F,Pan MH,Wan X,Lu Y,Zhang Y,Sun SC

Wed Mar 30 00:00:00 EDT 2022

RCCD1 depletion attenuates TGF-β-induced EMT and cell migration by stabilizing cytoskeletal microtubules in NSCLC cells.

Cancer letters

Wu J,He Z,Yang XM,Li KL,Wang DL,Sun FL

Tue Aug 01 00:00:00 EDT 2017

RCCD1 depletion attenuates TGF-β-induced EMT and cell migration by stabilizing cytoskeletal microtubules in NSCLC cells.

Cancer letters

Wu J,He Z,Yang XM,Li KL,Wang DL,Sun FL

Tue Aug 01 00:00:00 EDT 2017

Human GW182 Paralogs Are the Central Organizers for RNA-Mediated Control of Transcription.

Cell reports

Hicks JA,Li L,Matsui M,Chu Y,Volkov O,Johnson KC,Corey DR

Tue Aug 15 00:00:00 EDT 2017

Human GW182 Paralogs Are the Central Organizers for RNA-Mediated Control of Transcription.

Cell reports

Hicks JA,Li L,Matsui M,Chu Y,Volkov O,Johnson KC,Corey DR

Tue Aug 15 00:00:00 EDT 2017

Human GW182 Paralogs Are the Central Organizers for RNA-Mediated Control of Transcription.

Cell reports

Hicks JA,Li L,Matsui M,Chu Y,Volkov O,Johnson KC,Corey DR

Tue Aug 15 00:00:00 EDT 2017

Human GW182 Paralogs Are the Central Organizers for RNA-Mediated Control of Transcription.

Cell reports

Hicks JA,Li L,Matsui M,Chu Y,Volkov O,Johnson KC,Corey DR

Tue Aug 15 00:00:00 EDT 2017

Chemical inhibition of NAT10 corrects defects of laminopathic cells.

Science (New York, N.Y.)

Larrieu D,Britton S,Demir M,Rodriguez R,Jackson SP

Fri May 02 00:00:00 EDT 2014

Chemical inhibition of NAT10 corrects defects of laminopathic cells.

Science (New York, N.Y.)

Larrieu D,Britton S,Demir M,Rodriguez R,Jackson SP

Fri May 02 00:00:00 EDT 2014

Chemical inhibition of NAT10 corrects defects of laminopathic cells.

Science (New York, N.Y.)

Larrieu D,Britton S,Demir M,Rodriguez R,Jackson SP

Fri May 02 00:00:00 EDT 2014

Chemical inhibition of NAT10 corrects defects of laminopathic cells.

Science (New York, N.Y.)

Larrieu D,Britton S,Demir M,Rodriguez R,Jackson SP

Fri May 02 00:00:00 EDT 2014

Bioinformatics

Protein Aliases: 18S rRNA cytosine acetyltransferase; hALP; N-acetyltransferase 10; N-acetyltransferase 10 (GCN5-related); N-acetyltransferase-like protein; novel putative ATPase domain containing protein; RNA cytidine acetyltransferase

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Gene Aliases: AI429152; ALP; KIAA1709; NAT10; NET43; RGD1306717

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UniProt ID: (Human) Q9H0A0, (Mouse) Q8K224

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Entrez Gene ID: (Human) 55226, (Mouse) 98956, (Rat) 311257

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Function(s)

Process(es)

rRNA modification biological_process negative regulation of telomere maintenance via telomerase ribosomal small subunit biogenesis tRNA acetylation metabolic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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NAT10 Polyclonal Antibody

NAT10 Antibody (13365-1-AP) in ICC/IF

Product Details

13365-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

RNA Immunoprecipitation (RIP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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