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NCoR1 Antibody (PA5-142489) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of NCoR1 in Jurkat cells using NCoR1 Polyclonal Antibody (Product # PA5-142489). Cells were fixed with paraformaldehyde, permeabilized with 0.15% Triton, and incubated with the primary antibody for 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing nucleoli and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).
Product Details
PA5-142489
Product Specifications
Species Reactivity
Human
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Peptide with sequence C-SYDNIKNAREGTRSP.
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Ammonium sulfate precipitation
Storage buffer
TBS, pH 7.3, with 0.5% BSA
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2933136
Product Specific Information
This antibody is tested in Peptide ELISA: antibody detection limit dilution 1:16,000.
Target Information
This gene encodes a protein that mediates ligand-independent transcription repression of thyroid-hormone and retinoic-acid receptors by promoting chromatin condensation and preventing access of the transcription machinery. It is part of a complex which also includes histone deacetylases and transcriptional regulators similar to the yeast protein Sin3p. This gene is located between the Charcot-Marie-Tooth and Smith-Magenis syndrome critical regions on chromosome 17. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 17 and 20.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: h KIAA1047; hCIT529I10; MGC104216; N-CoR; nuclear receptor corepressor; Nuclear receptor corepressor 1; protein phosphatase 1, regulatory subunit 109; thyroid hormone- and retinoic acid receptor-associated corepressor 1
Gene Aliases: hN-CoR; KIAA1047; N-CoR; N-CoR1; NCOR1; PPP1R109; TRAC1
UniProt ID: (Human) O75376
Entrez Gene ID: (Human) 9611
RNA polymerase II regulatory region DNA binding
RNA polymerase II activating transcription factor binding
chromatin binding
transcription corepressor activity
protein binding
ligand-dependent nuclear receptor binding
protein domain specific binding
estrogen receptor binding
nuclear hormone receptor binding
histone deacetylase binding
peroxisome proliferator activated receptor binding
transcription regulatory region DNA binding
retinoid X receptor binding
thyroid hormone receptor binding
chromatin/chromatin-binding, or -regulatory protein
negative regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
skeletal muscle tissue development
lactation
circadian rhythm
positive regulation of cell proliferation
covalent chromatin modification
cerebellum development
cellular lipid metabolic process
negative regulation of JNK cascade
spindle assembly
regulation of glycolytic process by negative regulation of transcription from RNA polymerase II promoter
regulation of lipid transport by negative regulation of transcription from RNA polymerase II promoter
negative regulation of production of miRNAs involved in gene silencing by miRNA
regulation of fatty acid transport
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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