Proteintech

NDE1 Polyclonal Antibody

NDE1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 17

NDE1 Antibody (10233-1-AP) in ICC/IF

Immunofluorescent analysis of MCF-7 cells, using NDE1 antibody 10233-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

NDE1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

NDE1 Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))

NDE1 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

10233-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 24 publications 24 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunohistochemistry (PFA fixed) (IHC (PFA))

1:100

Immunocytochemistry (ICC/IF)

1:20-1:200

View 18 publications 18 publications

Immunoprecipitation (IP)

0.5-4.0 µg

View 2 publications 2 publications

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

NDE1 Fusion Protein Ag0336 (41-282 aa encoded by BC001421)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.45 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

This antibody is a rabbit polyclonal antibody raised against a peptide mapping within human NDE1.

Immunogen sequence: EGSREYEAE LETQLQQIET RNRDLLSENN RLRMELETIK EKFEVQHSEG YRQISALEDD LAQTKAIKDQ LQKYIRELEQ ANDDLERAKR ATIMSLEDFE QRLNQAIERN AFLESELDEK ENLLESVQRL KDEARDLRQE LAVQQKQEKP RTPMPSSVEA ERTDTAVQAT GSVPSTPIAH RGPSSSLNTP GSFRRGLDDS TGGTPLTPAA RISALNIVGD LLRKVGALES KLASCRNLVY DQS (41-282 aa encoded by BC001421)

Target Information

NDE1 is s a member of the nuclear distribution E (NudE) family of proteins. It is localized at the centrosome and interacts with other centrosome components as part of a multiprotein complex that regulates dynein function. NDE1 protein plays an essential role in microtubule organization, mitosis and neuronal migration. Mutations to the gene lead to Lissencephaly 4 (LIS4) and Microhydranencephaly (MHAC).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The meiotic LINC complex component KASH5 is an activating adaptor for cytoplasmic dynein.

The Journal of cell biology

Garner KEL,Salter A,Lau CK,Gurusaran M,Villemant CM,Granger EP,McNee G,Woodman PG,Davies OR,Burke BE,Allan VJ

Mon May 01 00:00:00 EDT 2023

The mechanism of acentrosomal spindle assembly in human oocytes.

Science (New York, N.Y.)

Wu T,Dong J,Fu J,Kuang Y,Chen B,Gu H,Luo Y,Gu R,Zhang M,Li W,Dong X,Sun X,Sang Q,Wang L

Fri Nov 18 00:00:00 EST 2022

Nde1 is required for heterochromatin compaction and stability in neocortical neurons.

iScience

Chomiak AA,Guo Y,Kopsidas CA,McDaniel DP,Lowe CC,Pan H,Zhou X,Zhou Q,Doughty ML,Feng Y

Fri Jun 17 00:00:00 EDT 2022

Nde1 is required for heterochromatin compaction and stability in neocortical neurons.

iScience

Chomiak AA,Guo Y,Kopsidas CA,McDaniel DP,Lowe CC,Pan H,Zhou X,Zhou Q,Doughty ML,Feng Y

Fri Jun 17 00:00:00 EDT 2022

CBP-HSF2 structural and functional interplay in Rubinstein-Taybi neurodevelopmental disorder.

Nature communications

de Thonel A,Ahlskog JK,Daupin K,Dubreuil V,Berthelet J,Chaput C,Pires G,Leonetti C,Abane R,Barris LC,Leray I,Aalto AL,Naceri S,Cordonnier M,Benasolo C,Sanial M,Duchateau A,Vihervaara A,Puustinen MC,Miozzo F,Fergelot P,Lebigot É,Verloes A,Gressens P,Lacombe D,Gobbo J,Garrido C,Westerheide SD,David L,Petitjean M,Taboureau O,Rodrigues-Lima F,Passemard S,Sabéran-Djoneidi D,Nguyen L,Lancaster M,Sistonen L,Mezger V

Wed Nov 16 00:00:00 EST 2022

Phosphorylation and Pin1 binding to the LIC1 subunit selectively regulate mitotic dynein functions.

The Journal of cell biology

Kumari A,Kumar C,Pergu R,Kumar M,Mahale SP,Wasnik N,Mylavarapu SVS

Mon Dec 06 00:00:00 EST 2021

Behavioral Deficits in Mice with Postnatal Disruption of Ndel1 in Forebrain Excitatory Neurons: Implications for Epilepsy and Neuropsychiatric Disorders.

Cerebral cortex communications

Gavrilovici C,Jiang Y,Kiroski I,Sterley TL,Vandal M,Bains J,Park SK,Rho JM,Teskey GC,Nguyen MD

Tue Feb 23 00:00:00 EST 2021

Cilium induction triggers differentiation of glioma stem cells.

Cell reports

Goranci-Buzhala G,Mariappan A,Ricci-Vitiani L,Josipovic N,Pacioni S,Gottardo M,Ptok J,Schaal H,Callaini G,Rajalingam K,Dynlacht B,Hadian K,Papantonis A,Pallini R,Gopalakrishnan J

Tue Sep 07 00:00:00 EDT 2021

Cilium induction triggers differentiation of glioma stem cells.

Cell reports

Goranci-Buzhala G,Mariappan A,Ricci-Vitiani L,Josipovic N,Pacioni S,Gottardo M,Ptok J,Schaal H,Callaini G,Rajalingam K,Dynlacht B,Hadian K,Papantonis A,Pallini R,Gopalakrishnan J

Tue Sep 07 00:00:00 EDT 2021

FBW7 couples structural integrity with functional output of primary cilia.

Communications biology

Petsouki E,Gerakopoulos V,Szeto N,Chang W,Humphrey MB,Tsiokas L

Mon Sep 13 00:00:00 EDT 2021

Posterior Neocortex-Specific Regulation of Neuronal Migration by CEP85L Identifies Maternal Centriole-Dependent Activation of CDK5.

Neuron

Kodani A,Kenny C,Lai A,Gonzalez DM,Stronge E,Sejourne GM,Isacco L,Partlow JN,O'Donnell A,McWalter K,Byrne AB,Barkovich AJ,Yang E,Hill RS,Gawlinski P,Wiszniewski W,Cohen JS,Fatemi SA,Baranano KW,Sahin M,Vossler DG,Yuskaitis CJ,Walsh CA

Wed Apr 22 00:00:00 EDT 2020

NRF2 negatively regulates primary ciliogenesis and hedgehog signaling.

PLoS biology

Liu P,Dodson M,Fang D,Chapman E,Zhang DD

Sat Feb 01 00:00:00 EST 2020

Phenylephrine alleviates ¹³¹I damage in submandibular gland through promoting endogenous stem cell regeneration via lissencephaly-1 upregulation.

Toxicology and applied pharmacology

Wang XY,Yu J,Zhang Y,Zhang FY,Liu KJ,Xiang B

Mon Jun 01 00:00:00 EDT 2020

Phenylephrine alleviates ¹³¹I damage in submandibular gland through promoting endogenous stem cell regeneration via lissencephaly-1 upregulation.

Toxicology and applied pharmacology

Wang XY,Yu J,Zhang Y,Zhang FY,Liu KJ,Xiang B

Mon Jun 01 00:00:00 EDT 2020

CENP-F stabilizes kinetochore-microtubule attachments and limits dynein stripping of corona cargoes.

The Journal of cell biology

Auckland P,Roscioli E,Coker HLE,McAinsh AD

Mon May 04 00:00:00 EDT 2020

The DISC1-Girdin complex - a missing link in signaling to the T cell cytoskeleton.

Journal of cell science

Maskalenko N,Nath S,Ramakrishnan A,Anikeeva N,Sykulev Y,Poenie M

Mon Jul 06 00:00:00 EDT 2020

Loss of polycystins suppresses deciliation via the activation of the centrosomal integrity pathway.

Life science alliance

Gerakopoulos V,Ngo P,Tsiokas L

Tue Sep 01 00:00:00 EDT 2020

A liquid-like spindle domain promotes acentrosomal spindle assembly in mammalian oocytes.

Science (New York, N.Y.)

So C,Seres KB,Steyer AM,Mönnich E,Clift D,Pejkovska A,Möbius W,Schuh M

Fri Jun 28 00:00:00 EDT 2019

Extraciliary roles of the ciliopathy protein JBTS17 in mitosis and neurogenesis.

Annals of neurology

Hong H,Joo K,Park SM,Seo J,Kim MH,Shin E,Cheong HI,Lee JH,Kim J

Mon Jul 01 00:00:00 EDT 2019

Neddylation inhibitor MLN4924 suppresses cilia formation by modulating AKT1.

Protein & cell

Mao H,Tang Z,Li H,Sun B,Tan M,Fan S,Zhu Y,Sun Y

Tue Oct 01 00:00:00 EDT 2019

NDE1 positively regulates oligodendrocyte morphological differentiation.

Scientific reports

Shimizu S,Ishino Y,Tohyama M,Miyata S

Wed May 16 00:00:00 EDT 2018

NDE1 positively regulates oligodendrocyte morphological differentiation.

Scientific reports

Shimizu S,Ishino Y,Tohyama M,Miyata S

Wed May 16 00:00:00 EDT 2018

NDE1 positively regulates oligodendrocyte morphological differentiation.

Scientific reports

Shimizu S,Ishino Y,Tohyama M,Miyata S

Wed May 16 00:00:00 EDT 2018

NDE1 positively regulates oligodendrocyte morphological differentiation.

Scientific reports

Shimizu S,Ishino Y,Tohyama M,Miyata S

Wed May 16 00:00:00 EDT 2018

Lateral attachment of kinetochores to microtubules is enriched in prometaphase rosette and facilitates chromosome alignment and bi-orientation establishment.

Scientific reports

Itoh G,Ikeda M,Iemura K,Amin MA,Kuriyama S,Tanaka M,Mizuno N,Osakada H,Haraguchi T,Tanaka K

Thu Mar 01 00:00:00 EST 2018

Lateral attachment of kinetochores to microtubules is enriched in prometaphase rosette and facilitates chromosome alignment and bi-orientation establishment.

Scientific reports

Itoh G,Ikeda M,Iemura K,Amin MA,Kuriyama S,Tanaka M,Mizuno N,Osakada H,Haraguchi T,Tanaka K

Thu Mar 01 00:00:00 EST 2018

The LIS1/NDE1 Complex Is Essential for FGF Signaling by Regulating FGF Receptor Intracellular Trafficking.

Cell reports

Liu L,Lu J,Li X,Wu A,Wu Q,Zhao M,Tang N,Song H

Tue Mar 20 00:00:00 EDT 2018

The LIS1/NDE1 Complex Is Essential for FGF Signaling by Regulating FGF Receptor Intracellular Trafficking.

Cell reports

Liu L,Lu J,Li X,Wu A,Wu Q,Zhao M,Tang N,Song H

Tue Mar 20 00:00:00 EDT 2018

The LIS1/NDE1 Complex Is Essential for FGF Signaling by Regulating FGF Receptor Intracellular Trafficking.

Cell reports

Liu L,Lu J,Li X,Wu A,Wu Q,Zhao M,Tang N,Song H

Tue Mar 20 00:00:00 EDT 2018

The LIS1/NDE1 Complex Is Essential for FGF Signaling by Regulating FGF Receptor Intracellular Trafficking.

Cell reports

Liu L,Lu J,Li X,Wu A,Wu Q,Zhao M,Tang N,Song H

Tue Mar 20 00:00:00 EDT 2018

The NDE1 genomic locus can affect treatment of psychiatric illness through gene expression changes related to microRNA-484.

Open biology

Bradshaw NJ,Ukkola-Vuoti L,Pankakoski M,Zheutlin AB,Ortega-Alonso A,Torniainen-Holm M,Sinha V,Therman S,Paunio T,Suvisaari J,Lönnqvist J,Cannon TD,Haukka J,Hennah W

Wed Nov 01 00:00:00 EDT 2017

The NDE1 genomic locus can affect treatment of psychiatric illness through gene expression changes related to microRNA-484.

Open biology

Bradshaw NJ,Ukkola-Vuoti L,Pankakoski M,Zheutlin AB,Ortega-Alonso A,Torniainen-Holm M,Sinha V,Therman S,Paunio T,Suvisaari J,Lönnqvist J,Cannon TD,Haukka J,Hennah W

Wed Nov 01 00:00:00 EDT 2017

CPAP promotes timely cilium disassembly to maintain neural progenitor pool.

The EMBO journal

Gabriel E,Wason A,Ramani A,Gooi LM,Keller P,Pozniakovsky A,Poser I,Noack F,Telugu NS,Calegari F,Šarić T,Hescheler J,Hyman AA,Gottardo M,Callaini G,Alkuraya FS,Gopalakrishnan J

Fri Apr 15 00:00:00 EDT 2016

CPAP promotes timely cilium disassembly to maintain neural progenitor pool.

The EMBO journal

Gabriel E,Wason A,Ramani A,Gooi LM,Keller P,Pozniakovsky A,Poser I,Noack F,Telugu NS,Calegari F,Šarić T,Hescheler J,Hyman AA,Gottardo M,Callaini G,Alkuraya FS,Gopalakrishnan J

Fri Apr 15 00:00:00 EDT 2016

CPAP promotes timely cilium disassembly to maintain neural progenitor pool.

The EMBO journal

Gabriel E,Wason A,Ramani A,Gooi LM,Keller P,Pozniakovsky A,Poser I,Noack F,Telugu NS,Calegari F,Šarić T,Hescheler J,Hyman AA,Gottardo M,Callaini G,Alkuraya FS,Gopalakrishnan J

Fri Apr 15 00:00:00 EDT 2016

CPAP promotes timely cilium disassembly to maintain neural progenitor pool.

The EMBO journal

Gabriel E,Wason A,Ramani A,Gooi LM,Keller P,Pozniakovsky A,Poser I,Noack F,Telugu NS,Calegari F,Šarić T,Hescheler J,Hyman AA,Gottardo M,Callaini G,Alkuraya FS,Gopalakrishnan J

Fri Apr 15 00:00:00 EDT 2016

Ndel1 suppresses ciliogenesis in proliferating cells by regulating the trichoplein-Aurora A pathway.

The Journal of cell biology

Inaba H,Goto H,Kasahara K,Kumamoto K,Yonemura S,Inoko A,Yamano S,Wanibuchi H,He D,Goshima N,Kiyono T,Hirotsune S,Inagaki M

Mon Feb 15 00:00:00 EST 2016

Ndel1 suppresses ciliogenesis in proliferating cells by regulating the trichoplein-Aurora A pathway.

The Journal of cell biology

Inaba H,Goto H,Kasahara K,Kumamoto K,Yonemura S,Inoko A,Yamano S,Wanibuchi H,He D,Goshima N,Kiyono T,Hirotsune S,Inagaki M

Mon Feb 15 00:00:00 EST 2016

Ndel1 suppresses ciliogenesis in proliferating cells by regulating the trichoplein-Aurora A pathway.

The Journal of cell biology

Inaba H,Goto H,Kasahara K,Kumamoto K,Yonemura S,Inoko A,Yamano S,Wanibuchi H,He D,Goshima N,Kiyono T,Hirotsune S,Inagaki M

Mon Feb 15 00:00:00 EST 2016

Ndel1 suppresses ciliogenesis in proliferating cells by regulating the trichoplein-Aurora A pathway.

The Journal of cell biology

Inaba H,Goto H,Kasahara K,Kumamoto K,Yonemura S,Inoko A,Yamano S,Wanibuchi H,He D,Goshima N,Kiyono T,Hirotsune S,Inagaki M

Mon Feb 15 00:00:00 EST 2016

Dynein Separately Partners with NDE1 and Dynactin To Orchestrate T Cell Focused Secretion.

Journal of immunology (Baltimore, Md. : 1950)

Nath S,Christian L,Tan SY,Ki S,Ehrlich LI,Poenie M

Thu Sep 15 00:00:00 EDT 2016

Dynein Separately Partners with NDE1 and Dynactin To Orchestrate T Cell Focused Secretion.

Journal of immunology (Baltimore, Md. : 1950)

Nath S,Christian L,Tan SY,Ki S,Ehrlich LI,Poenie M

Thu Sep 15 00:00:00 EDT 2016

N-Acetyl-D-Glucosamine Kinase Interacts with Dynein-Lis1-NudE1 Complex and Regulates Cell Division.

Molecules and cells

Sharif SR,Islam A,Moon IS

Thu Sep 01 00:00:00 EDT 2016

N-Acetyl-D-Glucosamine Kinase Interacts with Dynein-Lis1-NudE1 Complex and Regulates Cell Division.

Molecules and cells

Sharif SR,Islam A,Moon IS

Thu Sep 01 00:00:00 EDT 2016

NDE1 and GSK3β Associate with TRAK1 and Regulate Axonal Mitochondrial Motility: Identification of Cyclic AMP as a Novel Modulator of Axonal Mitochondrial Trafficking.

ACS chemical neuroscience

Ogawa F,Murphy LC,Malavasi EL,O'Sullivan ST,Torrance HS,Porteous DJ,Millar JK

Wed May 18 00:00:00 EDT 2016

NDE1 and GSK3β Associate with TRAK1 and Regulate Axonal Mitochondrial Motility: Identification of Cyclic AMP as a Novel Modulator of Axonal Mitochondrial Trafficking.

ACS chemical neuroscience

Ogawa F,Murphy LC,Malavasi EL,O'Sullivan ST,Torrance HS,Porteous DJ,Millar JK

Wed May 18 00:00:00 EDT 2016

NDE1 and GSK3β Associate with TRAK1 and Regulate Axonal Mitochondrial Motility: Identification of Cyclic AMP as a Novel Modulator of Axonal Mitochondrial Trafficking.

ACS chemical neuroscience

Ogawa F,Murphy LC,Malavasi EL,O'Sullivan ST,Torrance HS,Porteous DJ,Millar JK

Wed May 18 00:00:00 EDT 2016

NDE1 and GSK3β Associate with TRAK1 and Regulate Axonal Mitochondrial Motility: Identification of Cyclic AMP as a Novel Modulator of Axonal Mitochondrial Trafficking.

ACS chemical neuroscience

Ogawa F,Murphy LC,Malavasi EL,O'Sullivan ST,Torrance HS,Porteous DJ,Millar JK

Wed May 18 00:00:00 EDT 2016

Cell cycle-dependent ubiquitylation and destruction of NDE1 by CDK5-FBW7 regulates ciliary length.

The EMBO journal

Maskey D,Marlin MC,Kim S,Kim S,Ong EC,Li G,Tsiokas L

Thu Oct 01 00:00:00 EDT 2015

Cell cycle-dependent ubiquitylation and destruction of NDE1 by CDK5-FBW7 regulates ciliary length.

The EMBO journal

Maskey D,Marlin MC,Kim S,Kim S,Ong EC,Li G,Tsiokas L

Thu Oct 01 00:00:00 EDT 2015

Cell cycle-dependent ubiquitylation and destruction of NDE1 by CDK5-FBW7 regulates ciliary length.

The EMBO journal

Maskey D,Marlin MC,Kim S,Kim S,Ong EC,Li G,Tsiokas L

Thu Oct 01 00:00:00 EDT 2015

Cell cycle-dependent ubiquitylation and destruction of NDE1 by CDK5-FBW7 regulates ciliary length.

The EMBO journal

Maskey D,Marlin MC,Kim S,Kim S,Ong EC,Li G,Tsiokas L

Thu Oct 01 00:00:00 EDT 2015

The scaffold protein Nde1 safeguards the brain genome during S phase of early neural progenitor differentiation.

eLife

Houlihan SL,Feng Y

Tue Sep 23 00:00:00 EDT 2014

The scaffold protein Nde1 safeguards the brain genome during S phase of early neural progenitor differentiation.

eLife

Houlihan SL,Feng Y

Tue Sep 23 00:00:00 EDT 2014

Nde1-mediated inhibition of ciliogenesis affects cell cycle re-entry.

Nature cell biology

Kim S,Zaghloul NA,Bubenshchikova E,Oh EC,Rankin S,Katsanis N,Obara T,Tsiokas L

Fri Apr 01 00:00:00 EDT 2011

Nde1-mediated inhibition of ciliogenesis affects cell cycle re-entry.

Nature cell biology

Kim S,Zaghloul NA,Bubenshchikova E,Oh EC,Rankin S,Katsanis N,Obara T,Tsiokas L

Fri Apr 01 00:00:00 EDT 2011

Nde1-mediated inhibition of ciliogenesis affects cell cycle re-entry.

Nature cell biology

Kim S,Zaghloul NA,Bubenshchikova E,Oh EC,Rankin S,Katsanis N,Obara T,Tsiokas L

Fri Apr 01 00:00:00 EDT 2011

Nde1-mediated inhibition of ciliogenesis affects cell cycle re-entry.

Nature cell biology

Kim S,Zaghloul NA,Bubenshchikova E,Oh EC,Rankin S,Katsanis N,Obara T,Tsiokas L

Fri Apr 01 00:00:00 EDT 2011

The essential role of centrosomal NDE1 in human cerebral cortex neurogenesis.