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Invitrogen
NDE1 Recombinant Superclonal Antibody
Advanced Verification
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 3
NDE1 Antibody (711424) in WB
Western blot analysis was performed on Tissue extracts (30 µg lysate) of Mouse brain (Lane 1), Rat brain (Lane 2) and whole cell extracts of Neuro2a (Lane 3), IMR32 (Lane 4), T98G (Lane 5), NIH/3T3 (Lane 6), HeLa (Lane 7) and HepG2 (Lane 8). The blots were probed with Anti-Nde1 Recombinant Rabbit Polyclonal Antibody (Product # 711424, 1 µg/mL). A 40 kDa band corresponding to Nde1 was observed across the cell lines and tissues tested. The blots were detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondar... View More
Product Details
711424
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Superclonal
Type
Antibody
Immunogen
Protein corresponding to human NDE1 (aa1-aa346)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2692258
Product Specific Information
This antibody is predicted to react with Monkey, Cat, and Horse.
Recombinant rabbit polyclonal antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit polyclonal antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.
Target Information
NDE1 is s a member of the nuclear distribution E (NudE) family of proteins. It is localized at the centrosome and interacts with other centrosome components as part of a multiprotein complex that regulates dynein function. NDE1 protein plays an essential role in microtubule organization, mitosis and neuronal migration. Mutations to the gene lead to Lissencephaly 4 (LIS4) and Microhydranencephaly (MHAC).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: LIS1-interacting protein NUDE1; LIS1-interacting protein NUDE1, rat homolog; nuclear distribution gene E homolog 1; Nuclear distribution protein nudE homolog 1; NudE; nudE nuclear distribution E homolog 1; nudE nuclear distribution gene E homolog 1; rNudE
Gene Aliases: 2810027M15Rik; AU042936; AW822251; HOM-TES-87; LIS4; MHAC; mNudE; NDE; NDE1; NUDE; NUDE1
UniProt ID: (Human) Q9NXR1, (Rat) Q9ES39, (Mouse) Q9CZA6
Entrez Gene ID: (Human) 54820, (Rat) 83836, (Mouse) 67203
establishment of mitotic spindle orientation
neuron migration
microtubule nucleation
chromosome segregation
mitotic centrosome separation
neuroblast proliferation
cell migration
cerebral cortex development
forebrain development
microtubule organizing center organization
vesicle transport along microtubule
centrosome duplication
cell division
establishment of chromosome localization
centrosome localization
cell cycle
mitotic nuclear division
multicellular organism development
nervous system development
cell differentiation
G2/M transition of mitotic cell cycle
sister chromatid cohesion
regulation of microtubule motor activity
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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