Invitrogen

NDUFS3 Monoclonal Antibody (3F9DD2)

4 Published Figures

14 References

View all (22) NDUFS3 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Published Figures (4)

FIGURE: 1 / 4

NDUFS3 Antibody (459130) in WB

Nutrients 2020 - Figure 5 HAE activated the mitochondrial biogenesis pathway in mice livers and hearts. Con, control; P407, poloxamer 407; P407 + HAE, poloxamer 407 plus HAE at 200 mg/kg/day. ( A ) Protein levels of liver PGC-1alpha and mitochondrial complex subunits I-V (left, western blot image; right, statistical analysis). ( B ) Liver mtDNA copy number. ( C ) Liver ATP level. ( D ) Protein levels of heart PGC-1alpha and... View More

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Product Details

459130

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5 µg/mL

View 11 publications 11 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

Immunocytochemistry (ICC/IF)

5-10 µg/mL

Flow Cytometry (Flow)

1 µg/mL

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Bovine, Human, Mouse, Rat

Published species

Horse, Human, Mouse, Rat

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

3F9DD2

Immunogen

Whole mitochondria from Human Heart

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

IgG fraction

Storage buffer

HEPES buffered saline

Contains

0.02% sodium azide

Storage conditions

4° C, do not freeze

Shipping conditions

Wet ice

RRID

AB_10376043

Product Specific Information

Use Isolated mitochondria from Human, Bovine Rat and Mouse hearts; Cultured Human fibroblasts; HeLa cells; Human colon and cerebellum tissues as positive control.

Target Information

This gene encodes one of the iron-sulfur protein components of mitochondrial NADH:ubiquinone oxidoreductase. Mutations in this gene are associated with Leigh syndrome resulting from mitochondrial complex I deficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Involvement of the mitochondrial nuclease EndoG in the regulation of cell proliferation through the control of reactive oxygen species.

Redox biology

Blasco N,Beà A,Barés G,Girón C,Navaridas R,Irazoki A,López-Lluch G,Zorzano A,Dolcet X,Llovera M,Sanchis D

459130 was used in Western Blotting to investigate the role of EndoG in cell proliferation.

Thu Oct 01 00:00:00 EDT 2020

Involvement of the mitochondrial nuclease EndoG in the regulation of cell proliferation through the control of reactive oxygen species.

Redox biology

Blasco N,Beà A,Barés G,Girón C,Navaridas R,Irazoki A,López-Lluch G,Zorzano A,Dolcet X,Llovera M,Sanchis D

459130 was used in Western Blotting to investigate the role of EndoG in cell proliferation.

Thu Oct 01 00:00:00 EDT 2020

Herba Houttuyniae Extract Benefits Hyperlipidemic Mice via Activation of the AMPK/PGC-1α/Nrf2 Cascade.

Nutrients

Cao K,Lv W,Liu X,Fan Y,Wang K,Feng Z,Liu J,Zang W,Xing L,Liu J

459130 was used in Western Blot to link the AMPK/PGC-1α/Nrf2 cascade to hyperlipidemia-induced liver and heart impairments and demonstrate the protective effect of HAE as an AMPK activator in the prevention of hyperlipidemia-related diseases.

Tue Jan 07 00:00:00 EST 2020

Herba Houttuyniae Extract Benefits Hyperlipidemic Mice via Activation of the AMPK/PGC-1α/Nrf2 Cascade.

Nutrients

Cao K,Lv W,Liu X,Fan Y,Wang K,Feng Z,Liu J,Zang W,Xing L,Liu J

Tue Jan 07 00:00:00 EST 2020

Protection from α-Synuclein induced dopaminergic neurodegeneration by overexpression of the mitochondrial import receptor TOM20.

NPJ Parkinson's disease

De Miranda BR,Rocha EM,Castro SL,Greenamyre JT

459130 was used in Immunohistochemistry-immunofluorescence to show α-synuclein-induced mitochondrial dysfunction, a pathogenic mechanism in Parkinson's disease, is prevented by TOM20 overexpression in adult rat brains.

Tue Dec 08 00:00:00 EST 2020

Protection from α-Synuclein induced dopaminergic neurodegeneration by overexpression of the mitochondrial import receptor TOM20.

NPJ Parkinson's disease

De Miranda BR,Rocha EM,Castro SL,Greenamyre JT

Tue Dec 08 00:00:00 EST 2020

Low abundance of NDUFV2 and NDUFS4 subunits of the hydrophilic complex I domain and VDAC1 predicts mammalian longevity.

Redox biology

Mota-Martorell N,Jove M,Pradas I,Sanchez I,Gómez J,Naudi A,Barja G,Pamplona R

459130 was used in Western Blotting to analyse complex I in heart tissue from 8 mammalian species with a longevity ranging from 3.5 to 46 years.

Wed Jul 01 00:00:00 EDT 2020

L-Arabinose Elicits Gut-Derived Hydrogen Production and Ameliorates Metabolic Syndrome in C57BL/6J Mice on High-Fat-Diet.

Nutrients

Zhao L,Wang Y,Zhang G,Zhang T,Lou J,Liu J

459130 was used in Western Blot to show oral L-arabinose elicited immediate and robust release of hydrogen in mice in a dose-and-time-dependent manner while alleviating high-fat-diet induced metabolic syndrome.

Fri Dec 13 00:00:00 EST 2019

Cathepsin G Inhibition by Serpinb1 and Serpinb6 Prevents Programmed Necrosis in Neutrophils and Monocytes and Reduces GSDMD-Driven Inflammation.

Cell reports

Burgener SS,Leborgne NGF,Snipas SJ,Salvesen GS,Bird PI,Benarafa C

459130 was used in Western Blotting to demonstrate that cytosolic serpins expressed in myeloid cells prevent cell death and regulate inflammatory responses by inhibiting CatG and alternative activation of GSDMD.

Tue Jun 18 00:00:00 EDT 2019

Astrocyte-specific DJ-1 overexpression protects against rotenone-induced neurotoxicity in a rat model of Parkinson's disease.

Neurobiology of disease

De Miranda BR,Rocha EM,Bai Q,El Ayadi A,Hinkle D,Burton EA,Timothy Greenamyre J

459130 was used in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to indicate that astrocyte-specific overexpression of hDJ-1 protects neighboring neurons against multiple pathologic features of Parkinson's disease and provides the first direct evidence in vivo of a cell non-autonomous neuroprotective function of astroglial DJ-1.

Sun Jul 01 00:00:00 EDT 2018

Sixty years old is the breakpoint of human frontal cortex aging.

Free radical biology & medicine

Cabré R,Naudí A,Dominguez-Gonzalez M,Ayala V,Jové M,Mota-Martorell N,Piñol-Ripoll G,Gil-Villar MP,Rué M,Portero-Otín M,Ferrer I,Pamplona R

459130 was used in western blot to discover that protein oxidative and glycoxidative damage significantly increases during human brain aging

Wed Feb 01 00:00:00 EST 2017

Enhanced GLUT4-Dependent Glucose Transport Relieves Nutrient Stress in Obese Mice Through Changes in Lipid and Amino Acid Metabolism.

Diabetes

Gurley JM,Ilkayeva O,Jackson RM,Griesel BA,White P,Matsuzaki S,Qaisar R,Van Remmen H,Humphries KM,Newgard CB,Olson AL

Published figure using NDUFS3 monoclonal antibody (Product # 459130) in Western Blot

Thu Dec 01 00:00:00 EST 2016

Calsequestrins in skeletal and cardiac muscle from adult Danio rerio.

Journal of muscle research and cell motility

Furlan S,Mosole S,Murgia M,Nagaraj N,Argenton F,Volpe P,Nori A

459130 was used in western blot to analyze Danio rerio for calsequestrins in cardiac and skeletal muscle

Fri Apr 01 00:00:00 EDT 2016

Cysteine dietary supplementation reverses the decrease in mitochondrial ROS production at complex I induced by methionine restriction.

Journal of bioenergetics and biomembranes

Gomez A,Gomez J,Lopez Torres M,Naudi A,Mota-Martorell N,Pamplona R,Barja G

459130 was used in western blot to investigate cysteine and methionine dietary supplementation in aging rodents.

Mon Jun 01 00:00:00 EDT 2015

Insulin and IGF-1 improve mitochondrial function in a PI-3K/Akt-dependent manner and reduce mitochondrial generation of reactive oxygen species in Huntington's disease knock-in striatal cells.

Free radical biology & medicine

Ribeiro M,Rosenstock TR,Oliveira AM,Oliveira CR,Rego AC

459130 was used in western blot to examine the role of insulin and IGF-1 on the mitochondria of striatal cells derived from HD knock-in mice.

Mon Sep 01 00:00:00 EDT 2014

Independent and additive effects of atenolol and methionine restriction on lowering rat heart mitochondria oxidative stress.

Journal of bioenergetics and biomembranes

Sanchez-Roman I,Gomez A,Naudí A,Jove M,Gómez J,Lopez-Torres M,Pamplona R,Barja G

459130 was used in western blot to study the effects on rat cardiac mitochondrial oxidative stress of simultaneous treatment with atenolol plus methionine restriction.

Sun Jun 01 00:00:00 EDT 2014

IKKα and alternative NF-κB regulate PGC-1β to promote oxidative muscle metabolism.

The Journal of cell biology

Bakkar N,Ladner K,Canan BD,Liyanarachchi S,Bal NC,Pant M,Periasamy M,Li Q,Janssen PM,Guttridge DC

Published figure using NDUFS3 monoclonal antibody (Product # 459130) in Western Blot

Mon Feb 20 00:00:00 EST 2012

Bioinformatics

Protein Aliases: CI-30kD; complex I 30kDa subunit; Complex I-30kD; NADH dehydrogenase (ubiquinone) Fe-S protein 3, 30kDa (NADH-coenzyme Q reductase); NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial; NADH dehydrogenase-ubiquinone 30 kDa subunit; NADH-ubiquinone oxidoreductase 30 kDa subunit

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Gene Aliases: 0610010M09Rik; CI-30; CI-30kD; NDUFS3

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UniProt ID: (Bovine) P23709, (Human) O75489, (Mouse) Q9DCT2

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Entrez Gene ID: (Bovine) 287327, (Human) 4722, (Mouse) 68349, (Rat) 295923

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Function(s)

NADH dehydrogenase activity NADH dehydrogenase (ubiquinone) activity protein binding electron carrier activity oxidoreductase activity oxidoreductase activity, acting on NAD(P)H molecular_function dehydrogenase oxidoreductase metabolite interconversion enzyme

Process(es)

negative regulation of cell growth oxidation-reduction process reactive oxygen species metabolic process negative regulation of intrinsic apoptotic signaling pathway mitochondrial electron transport, NADH to ubiquinone substantia nigra development mitochondrial respiratory chain complex I assembly transport biological_process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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NDUFS3 Monoclonal Antibody (3F9DD2)

NDUFS3 Antibody (459130) in WB

Product Details

459130

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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