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NEDD4 Polyclonal Antibody

NEDD4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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NEDD4 Antibody (21698-1-AP) in IHC (P)

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using (Product # 21698-1-AP) (NEDD4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

NEDD4 Antibody in Immunoprecipitation (IP)

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Product Details

21698-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2400

View 26 publications 26 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

View 4 publications 4 publications

Immunoprecipitation (IP)

0.5-4.0 µg

View 5 publications 5 publications

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

NEDD4 Fusion Protein Ag16291 (970-1319 aa encoded by BC152452)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.22 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

This antibody can recognize NEDD4-1 and NEDD4-2.

Immunogen sequence: TVLEDSYRR IMGVKRADFL KARLWIEFDG EKGLDYGGVA REWFFLISKE MFNPYYGLFE YSATDNYTLQ INPNSGLCNE DHLSYFKFIG RVAGMAVYHG KLLDGFFIRP FYKMMLHKPI TLHDMESVDS EYYNSLRWIL ENDPTELDLR FIIDEELFGQ THQHELKNGG SEIVVTNKNK KEYIYLVIQW RFVNRIQKQM AAFKEGFFEL IPQDLIKIFD ENELELLMCG LGDVDVNDWR EHTKYKNGYS ANHQVIQWFW KAVLMMDSEK RIRLLQFVTG TSRVPMNGFA ELYGSNGPQS FTVEQWGTPE KLPRAHTCFN RLDLPPYESF EELWDKLQMA IENTQGFDGV D (970-1319 aa encoded by BC152452)

Target Information

The E3 ubiquitin-protein ligase NEDD4 is a Ubl conjugation pathway protein. It contains one HECT (E6AP-type E3 ubiquitin-protein ligase) domain and four WW domains. It is involved in the pathway leading to the degradation of VEGFR-2/KDFR, independently of its ubiquitin-ligase activity. It acts as a potential proto-oncogene that negatively regulates PTEN via ubiquitination, a paradigm analogous to that of Mdm2 and p53. It is also reported to be involved in the budding of many viruses and is the regulator of epithelial Na+ channels, which lead to arterial hypertension. NEDD4 protein is localized to the sarcolemmal region of muscle fibers. Increase in NEDD4 protein expression leads to unloaded and denervated muscles as well as a decrease in Notch1 expression. Hence it acts as down regulator of Notch-1 gene expression.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Xinbao Pill attenuated chronic heart failure by suppressing the ubiquitination of β-adrenergic receptors.

Phytomedicine : international journal of phytotherapy and phytopharmacology

Tian X,Huang Z,Wang Y,Qi X,Wang D,Liu Z,Cheng Y

Sat Jul 01 00:00:00 EDT 2023

Xinbao Pill attenuated chronic heart failure by suppressing the ubiquitination of β-adrenergic receptors.

Phytomedicine : international journal of phytotherapy and phytopharmacology

Tian X,Huang Z,Wang Y,Qi X,Wang D,Liu Z,Cheng Y

Sat Jul 01 00:00:00 EDT 2023

hCINAP regulates the differentiation of embryonic stem cells by regulating NEDD4 liquid-liquid phase-separation-mediated YAP1 activation.

Cell reports

Zhuge R,Wang C,Wang J,Yu S,Liao L,Zheng X

Tue Jan 31 00:00:00 EST 2023

hCINAP regulates the differentiation of embryonic stem cells by regulating NEDD4 liquid-liquid phase-separation-mediated YAP1 activation.

Cell reports

Zhuge R,Wang C,Wang J,Yu S,Liao L,Zheng X

Tue Jan 31 00:00:00 EST 2023

hCINAP regulates the differentiation of embryonic stem cells by regulating NEDD4 liquid-liquid phase-separation-mediated YAP1 activation.

Cell reports

Zhuge R,Wang C,Wang J,Yu S,Liao L,Zheng X

Tue Jan 31 00:00:00 EST 2023

Phosphorylation by IKKβ Promotes the Degradation of HMGCL via NEDD4 in Lung Cancer.

International journal of biological sciences

Zhong C,Xiong G,Yang H,Du X,Du J,Yao F,Fang W,Deng Y

Tue Mar 21 00:00:00 EDT 2023

Phosphorylation by IKKβ Promotes the Degradation of HMGCL via NEDD4 in Lung Cancer.

International journal of biological sciences

Zhong C,Xiong G,Yang H,Du X,Du J,Yao F,Fang W,Deng Y

Tue Mar 21 00:00:00 EDT 2023

NEDD4 ameliorates myocardial reperfusion injury by preventing macrophages pyroptosis.

Cell communication and signaling : CCS

Sun W,Lu H,Cui S,Zhao S,Yu H,Song H,Ruan Q,Zhang Y,Chu Y,Dong S

Thu Feb 02 00:00:00 EST 2023

CFDP1 promotes hepatocellular carcinoma progression through activating NEDD4/PTEN/PI3K/AKT signaling pathway.

Cancer medicine

Zhou Y,Qiu J,Liu S,Wang P,Ma D,Zhang G,Cao Y,Hu L,Wang Z,Wu J,Jiang C

Sun Jan 01 00:00:00 EST 2023

Mesenchymal stem cells-derived small extracellular vesicles alleviate diabetic retinopathy by delivering NEDD4.

Stem cell research & therapy

Sun F,Sun Y,Zhu J,Wang X,Ji C,Zhang J,Chen S,Yu Y,Xu W,Qian H

Fri Jul 15 00:00:00 EDT 2022

Mesenchymal stem cells-derived small extracellular vesicles alleviate diabetic retinopathy by delivering NEDD4.

Stem cell research & therapy

Sun F,Sun Y,Zhu J,Wang X,Ji C,Zhang J,Chen S,Yu Y,Xu W,Qian H

Fri Jul 15 00:00:00 EDT 2022

Mesenchymal stem cells-derived small extracellular vesicles alleviate diabetic retinopathy by delivering NEDD4.

Stem cell research & therapy

Sun F,Sun Y,Zhu J,Wang X,Ji C,Zhang J,Chen S,Yu Y,Xu W,Qian H

Fri Jul 15 00:00:00 EDT 2022

NEDD4 attenuates phosgene-induced acute lung injury through the inhibition of Notch1 activation.

Journal of cellular and molecular medicine

Shao Y,Jiang Z,He D,Shen J

Sun May 01 00:00:00 EDT 2022

NEDD4 attenuates phosgene-induced acute lung injury through the inhibition of Notch1 activation.

Journal of cellular and molecular medicine

Shao Y,Jiang Z,He D,Shen J

Sun May 01 00:00:00 EDT 2022

Identification of Marek's disease virus pUL56 homologue and analysis of critical amino acid stretches indispensable for its intracellular localization.

Virus research

Zhang J,Xu B,Ma D,Song J,Liu X,Wei P,Huang T

Sun May 01 00:00:00 EDT 2022

Hepatokine ERAP1 Disturbs Skeletal Muscle Insulin Sensitivity Via Inhibiting USP33-Mediated ADRB2 Deubiquitination.

Diabetes

Niu Y,Jiang H,Yin H,Wang F,Hu R,Hu X,Peng B,Shu Y,Li Z,Chen S,Guo F

Sun May 01 00:00:00 EDT 2022

PINCH-1 promotes IGF-1 receptor expression and skin cancer progression through inhibition of the GRB10-NEDD4 complex.

Theranostics

Wang X,Wang R,Jiang K,Zhu M,Guo L,Wu C

Tue Apr 12 00:00:00 EDT 2022

JAC1 suppresses proliferation of breast cancer through the JWA/p38/SMURF1/HER2 signaling.

Cell death discovery

Ren Y,Chen D,Zhai Z,Chen J,Li A,Liang Y,Zhou J

Mon Apr 19 00:00:00 EDT 2021

HECT E3 Ubiquitin Ligase Nedd4 Is Required for Antifungal Innate Immunity.

Journal of immunology (Baltimore, Md. : 1950)

Nuro-Gyina PK,Tang N,Guo H,Yan C,Zeng Q,Waldschmidt TJ,Zhang J

Sun Aug 01 00:00:00 EDT 2021

Samotolisib Attenuates Acute Liver Injury Through Inhibiting Caspase-11-Mediated Pyroptosis Via Regulating E3 Ubiquitin Ligase Nedd4.

Frontiers in pharmacology

Zhao YY,Wu DM,He M,Zhang F,Zhang T,Liu T,Li J,Li L,Xu Y

Tue Sep 07 00:00:00 EDT 2021

Human cytomegalovirus UL42 protein inhibits the degradation of glycoprotein B through inhibition of Nedd4 family ubiquitin E3 ligases.

Microbiology and immunology

Koshizuka T,Kondo H,Kato H,Takahashi K

Mon Nov 01 00:00:00 EDT 2021

Human cytomegalovirus UL42 protein inhibits the degradation of glycoprotein B through inhibition of Nedd4 family ubiquitin E3 ligases.

Microbiology and immunology

Koshizuka T,Kondo H,Kato H,Takahashi K

Mon Nov 01 00:00:00 EDT 2021

Human cytomegalovirus UL42 protein inhibits the degradation of glycoprotein B through inhibition of Nedd4 family ubiquitin E3 ligases.

Microbiology and immunology

Koshizuka T,Kondo H,Kato H,Takahashi K

Mon Nov 01 00:00:00 EDT 2021

Identification of a new natural biflavonoids against breast cancer cells induced ferroptosis via the mitochondrial pathway.

Bioorganic chemistry

Xie Y,Zhou X,Li J,Yao XC,Liu WL,Kang FH,Zou ZX,Xu KP,Xu PS,Tan GS

Thu Apr 01 00:00:00 EDT 2021

Tumor-derived exosomal miR-19b-3p facilitates M2 macrophage polarization and exosomal LINC00273 secretion to promote lung adenocarcinoma metastasis via Hippo pathway.

Clinical and translational medicine

Chen J,Zhang K,Zhi Y,Wu Y,Chen B,Bai J,Wang X

Wed Sep 01 00:00:00 EDT 2021

Tumor-derived exosomal miR-19b-3p facilitates M2 macrophage polarization and exosomal LINC00273 secretion to promote lung adenocarcinoma metastasis via Hippo pathway.

Clinical and translational medicine

Chen J,Zhang K,Zhi Y,Wu Y,Chen B,Bai J,Wang X

Wed Sep 01 00:00:00 EDT 2021

Distinct and Overlapping Roles of Hippo Effectors YAP and TAZ During Human and Mouse Hepatocarcinogenesis.

Cellular and molecular gastroenterology and hepatology

Wang H,Wang J,Zhang S,Jia J,Liu X,Zhang J,Wang P,Song X,Che L,Liu K,Ribback S,Cigliano A,Evert M,Wu H,Calvisi DF,Zeng Y,Chen X

Mon Mar 07 00:00:00 EST 2022

Multivesicular body sorting and the exosomal pathway are required for the release of rat hepatitis E virus from infected cells.

Virus research

Primadharsini PP,Nagashima S,Takahashi M,Kobayashi T,Nishiyama T,Nishizawa T,Yasuda J,Okamoto H

Sun Mar 01 00:00:00 EST 2020

Ubiquitin ligase CHIP regulates OTUD3 stability and suppresses tumour metastasis in lung cancer.

Cell death and differentiation

Zhang P,Li C,Li H,Yuan L,Dai H,Peng Z,Deng Z,Chang Z,Cui CP,Zhang L

Sun Nov 01 00:00:00 EDT 2020

YY1-regulated LINC00152 promotes triple negative breast cancer progression by affecting on stability of PTEN protein.

Biochemical and biophysical research communications

Shen X,Zhong J,Yu P,Zhao Q,Huang T

Tue Feb 05 00:00:00 EST 2019

YY1-regulated LINC00152 promotes triple negative breast cancer progression by affecting on stability of PTEN protein.

Biochemical and biophysical research communications

Shen X,Zhong J,Yu P,Zhao Q,Huang T

Tue Feb 05 00:00:00 EST 2019

Promotion of liver growth by CAR is accompanied by Akt pathway activation and FoxM1-Nedd4-mediated repression of PTEN.

Archives of biochemistry and biophysics

Yarushkin AA,Mazin ME,Pustylnyak YA,Prokopyeva EA,Pustylnyak VO

Sun Sep 15 00:00:00 EDT 2019

N-myc downstream-regulated gene 1 inhibits the proliferation of colorectal cancer through emulative antagonizing NEDD4-mediated ubiquitylation of p21.

Journal of experimental & clinical cancer research : CR

Zhang S,Yu C,Yang X,Hong H,Lu J,Hu W,Hao X,Li S,Aikemu B,Yang G,He Z,Zhang L,Xue P,Cai Z,Ma J,Zang L,Feng B,Yuan F,Sun J,Zheng M

Thu Dec 12 00:00:00 EST 2019

NEDD4 protects vascular endothelial cells against Angiotensin II-induced cell death via enhancement of XPO1-mediated nuclear export.

Experimental cell research

Xu J,Sheng Z,Li F,Wang S,Yuan Y,Wang M,Yu Z

Tue Oct 01 00:00:00 EDT 2019

Dynamic recruitment of ubiquitin to mutant huntingtin inclusion bodies.

Scientific reports

Juenemann K,Jansen AHP,van Riel L,Merkx R,Mulder MPC,An H,Statsyuk A,Kirstein J,Ovaa H,Reits EA

Tue Jan 23 00:00:00 EST 2018

Dynamic recruitment of ubiquitin to mutant huntingtin inclusion bodies.

Scientific reports

Juenemann K,Jansen AHP,van Riel L,Merkx R,Mulder MPC,An H,Statsyuk A,Kirstein J,Ovaa H,Reits EA

Tue Jan 23 00:00:00 EST 2018

Zbtb7b engages the long noncoding RNA Blnc1 to drive brown and beige fat development and thermogenesis.

Proceedings of the National Academy of Sciences of the United States of America

Li S,Mi L,Yu L,Yu Q,Liu T,Wang GX,Zhao XY,Wu J,Lin JD

Tue Aug 22 00:00:00 EDT 2017

Zbtb7b engages the long noncoding RNA Blnc1 to drive brown and beige fat development and thermogenesis.

Proceedings of the National Academy of Sciences of the United States of America

Li S,Mi L,Yu L,Yu Q,Liu T,Wang GX,Zhao XY,Wu J,Lin JD

Tue Aug 22 00:00:00 EDT 2017

Inhibition of ubiquitin proteasome function prevents monocrotaline-induced pulmonary arterial remodeling.

Life sciences

Zhu Y,Wu Y,Shi W,Wang J,Yan X,Wang Q,Liu Y,Yang L,Gao L,Li M

Wed Mar 15 00:00:00 EDT 2017

Inhibition of ubiquitin proteasome function prevents monocrotaline-induced pulmonary arterial remodeling.

Life sciences

Zhu Y,Wu Y,Shi W,Wang J,Yan X,Wang Q,Liu Y,Yang L,Gao L,Li M

Wed Mar 15 00:00:00 EDT 2017

Phosphatase and tensin homolog deleted on chromosome 10 degradation induced by NEDD4 promotes acquired erlotinib resistance in non-small-cell lung cancer.

Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine

Sun H,Ma H,Wang J,Xia L,Zhu G,Wang Z,Sun J,Chen Z

Sat Jul 01 00:00:00 EDT 2017

Phosphatase and tensin homolog deleted on chromosome 10 degradation induced by NEDD4 promotes acquired erlotinib resistance in non-small-cell lung cancer.

Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine

Sun H,Ma H,Wang J,Xia L,Zhu G,Wang Z,Sun J,Chen Z

Sat Jul 01 00:00:00 EDT 2017

O-GlcNAc regulates NEDD4-1 stability via caspase-mediated pathway.

Biochemical and biophysical research communications

Jiang K,Bai B,Ta Y,Zhang T,Xiao Z,Wang PG,Zhang L

Fri Mar 18 00:00:00 EDT 2016

O-GlcNAc regulates NEDD4-1 stability via caspase-mediated pathway.

Biochemical and biophysical research communications

Jiang K,Bai B,Ta Y,Zhang T,Xiao Z,Wang PG,Zhang L

Fri Mar 18 00:00:00 EDT 2016

Bioinformatics

Protein Aliases: Cell proliferation-inducing gene 53 protein; E3 ubiquitin-protein ligase NEDD4; HECT-type E3 ubiquitin transferase NEDD4; NEDD-4; Neural precursor cell expressed developmentally down-regulated protein 4; neural precursor cell expressed, developmentally down-regulated gene 4; neural precursor cell expressed, developmentally down-regulated gene 4A; neural precursor cell expressed, developmentally down-regulted gene 4; OTTHUMP00000217840; OTTHUMP00000217841; OTTHUMP00000217842; receptor-potentiating factor 1

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Gene Aliases: AA959633; AL023035; AU019897; E430025J12Rik; KIAA0093; mKIAA0093; Nedd-4; NEDD4; NEDD4-1; Nedd4a; PIG53; RPF1

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UniProt ID: (Human) P46934, (Rat) Q62940, (Mouse) P46935

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Entrez Gene ID: (Human) 4734, (Rat) 25489, (Mouse) 17999

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Function(s)

protein binding protein C-terminus binding ligase activity sodium channel inhibitor activity protein domain specific binding beta-2 adrenergic receptor binding ubiquitin binding phosphoserine binding phosphothreonine binding ubiquitin protein ligase activity RNA polymerase binding proline-rich region binding ubiquitin-protein transferase activity UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity ribosomal S6-glutamic acid ligase activity coenzyme F420-0 gamma-glutamyl ligase activity coenzyme F420-2 alpha-glutamyl ligase activity protein-glycine ligase activity protein-glycine ligase activity, initiating protein-glycine ligase activity, elongating tubulin-glycine ligase activity protein-glutamic acid ligase activity tubulin-glutamic acid ligase activity ubiquitin-protein ligase

Process(es)

adaptive immune response outflow tract morphogenesis endocardial cushion development protein monoubiquitination protein targeting to lysosome lysosomal transport neuromuscular junction development negative regulation of sodium ion transport negative regulation of transcription from RNA polymerase II promoter in response to UV-induced DNA damage positive regulation of phosphatidylinositol 3-kinase signaling response to denervation involved in regulation of muscle adaptation regulation of macroautophagy protein ubiquitination transmission of virus negative regulation of vascular endothelial growth factor receptor signaling pathway neuron projection development receptor internalization receptor catabolic process cellular response to UV regulation of ion transmembrane transport T cell activation regulation of membrane potential protein ubiquitination involved in ubiquitin-dependent protein catabolic process glucocorticoid receptor signaling pathway ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway development involved in symbiotic interaction positive regulation of protein catabolic process positive regulation of nucleocytoplasmic transport blood vessel morphogenesis regulation of dendrite morphogenesis regulation of synapse organization progesterone receptor signaling pathway response to calcium ion protein K63-linked ubiquitination regulation of potassium ion transmembrane transporter activity negative regulation of sodium ion transmembrane transporter activity negative regulation of transcription from RNA polymerase II promoter immune response nervous system development

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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NEDD4 Polyclonal Antibody

NEDD4 Antibody (21698-1-AP) in IHC (P)

Product Details

21698-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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