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Product Details
MA5-32294
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
SN73-03
Immunogen
Synthetic peptide within Human NEDD4-2 aa 926-975.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 40% Glycerol, 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2809579
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
NEDD4L is an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. NEDD4L inhibits TGF-beta signaling by triggering SMAD2 and TGFR1 ubiquitination and proteasome-dependent degradation. NEDD4L promotes ubiquitination and internalization of various plasma membrane channels such as ENaC, Nav1.2, Nav1.3, Nav1.5, Nav1.7, Nav1.8, Kv1.3, EAAT1 or CLC5. NEDD4L also promotes ubiquitination and degradation of SGK.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: E3 ubiquitin-protein ligase NEDD4-like; FLJ33870; HECT-type E3 ubiquitin transferase NED4L; Nedd4-2; NEDD4.2; neural precursor cell expressed, developmentally down-regulated gene 4b; ubiquitin-protein ligase Rsp5
Gene Aliases: 1300012C07Rik; hNEDD4-2; KIAA0439; NEDD4-2; NEDD4.2; Nedd4b; NEDD4L; NEDL3; RSP5
UniProt ID: (Human) Q96PU5
Entrez Gene ID: (Human) 23327, (Rat) 291553, (Mouse) 83814
ubiquitin-protein transferase activity
protein binding
potassium channel regulator activity
ligase activity
sodium channel regulator activity
potassium channel inhibitor activity
sodium channel inhibitor activity
ion channel binding
ubiquitin protein ligase activity
UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity
ribosomal S6-glutamic acid ligase activity
coenzyme F420-0 gamma-glutamyl ligase activity
coenzyme F420-2 alpha-glutamyl ligase activity
protein-glycine ligase activity
protein-glycine ligase activity, initiating
protein-glycine ligase activity, elongating
tubulin-glycine ligase activity
protein-glutamic acid ligase activity
tubulin-glutamic acid ligase activity
ubiquitin-protein ligase
negative regulation of transcription from RNA polymerase II promoter
protein polyubiquitination
regulation of membrane depolarization
protein monoubiquitination
sodium ion transport
cellular sodium ion homeostasis
excretion
response to metal ion
protein ubiquitination
viral life cycle
water homeostasis
ion transmembrane transport
regulation of ion transmembrane transport
regulation of protein catabolic process
regulation of membrane potential
protein ubiquitination involved in ubiquitin-dependent protein catabolic process
proteasome-mediated ubiquitin-dependent protein catabolic process
positive regulation of protein catabolic process
positive regulation of endocytosis
regulation of membrane repolarization
protein K48-linked ubiquitination
ventricular cardiac muscle cell action potential
regulation of potassium ion transmembrane transporter activity
negative regulation of potassium ion transmembrane transporter activity
negative regulation of potassium ion transmembrane transport
negative regulation of sodium ion transmembrane transport
positive regulation of dendrite extension
negative regulation of protein localization to cell surface
negative regulation of sodium ion transmembrane transporter activity
positive regulation of caveolin-mediated endocytosis
negative regulation of systemic arterial blood pressure
response to salt stress
positive regulation of sodium ion transport
negative regulation of sodium ion transport
ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway
regulation of bicellular tight junction assembly
positive regulation of cation channel activity
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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