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Invitrogen
NEFL Polyclonal Antibody
Advanced Verification
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 4
NEFL Antibody (PA1-10000) in ICC/IF
Immunofluorescent analysis of NEFL in rat cerebellum. The rat cerebellum section was obtained following transcardial perfusion of the rat with 4% paraformaldehyde, brain was post fixed for 24 hours, and cut to 45µM. Free-floating sections were stained with an NEFL polyclonal antibody (Product # PA1-10000) at a dilution of 1:2,000 in green, and costained with NeuN/Fox3 monoclonal antibody at a dilution of 1:5,000 in red. The NEFL polyclonal antibody (Product # PA1-10000) antibody labels perikarya and processes of neuronal cells, particularl... View More
Product Details
PA1-10000
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Human,
Mouse,
Pig,
Rat
Published species
Human
Host/Isotype
Chicken
/ IgY
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant human NF-L protein
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
PBS
Contains
0.02% sodium azide
Storage conditions
4° C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_1077181
Product Specific Information
PA1-10000 was made against a preparation of full length human recombinant NF-L protein. It binds NF-L from a variety of species including human, rat and mouse.
Target Information
Involved in the maintenance of neuronal caliber, neurofilaments are the intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. NF-L is the light or low molecular weight microfilament subunit and runs on SDS-PAGE gels at approximately 70 kDa. Neurofilament are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-H is the heavy or high molecular weight microfilament subunit and runs on SDS-PAGE gels in the range 180-220 kDa, with some variation in different species.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 68 kDa neurofilament protein; FLJ53642; light molecular weight neurofilament protein; Micro glutamic acid-rich protein; NEF3; neurofilament; neurofilament 3; Neurofilament light polypeptide; neurofilament protein L; neurofilament protein, light chain; neurofilament subunit NF-L; Neurofilament triplet L protein; neurofilament, light polypeptide; neurofilament, light polypeptide 68kDa; neurofilament-l; neurofilament-light; NF-L; NFM; NLC; OTTHUMP00000196614; protein phosphatase 1, regulatory subunit 110
Gene Aliases: AI847934; CMT1F; CMT2E; NEFL; NF-L; NF68; NFL; PPP1R110
UniProt ID: (Bovine) P02548, (Human) P07196, (Mouse) P08551, (Rat) P19527
Entrez Gene ID: (Bovine) 281348, (Human) 4747, (Pig) 100521224, (Mouse) 18039, (Rat) 83613
structural constituent of cytoskeleton
protein C-terminus binding
protein binding, bridging
identical protein binding
structural constituent of postsynaptic intermediate filament cytoskeleton
Ras guanyl-nucleotide exchange factor activity
protein binding
protein domain specific binding
phospholipase binding
protein heterodimerization activity
structural molecule activity
microtubule cytoskeleton organization
anterograde axonal transport
retrograde axonal transport
peripheral nervous system axon regeneration
axonal transport of mitochondrion
regulation of axon diameter
neurofilament bundle assembly
locomotion
negative regulation of neuron apoptotic process
intermediate filament organization
intermediate filament bundle assembly
neuron projection morphogenesis
positive regulation of axonogenesis
neuromuscular process controlling balance
neurofilament cytoskeleton organization
synapse maturation
postsynaptic intermediate filament cytoskeleton organization
MAPK cascade
response to toxic substance
spinal cord development
hippocampus development
cerebral cortex development
response to peptide hormone
positive regulation of GTPase activity
intermediate filament polymerization or depolymerization
protein polymerization
response to corticosterone
response to sodium arsenite
response to acrylamide
axon development
response to organic substance
response to organonitrogen compound
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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