Invitrogen

NEFL Monoclonal Antibody (DA2)

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

5 Published Figures

21 References

View all (78) NEFL antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

NEFL Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 14

NEFL Antibody (13-0400) in IHC (P)

Immunohistochemistry analysis of the neurofilament light chain showing staining in the filaments of paraffin-embedded mouse brain tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0) and microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 m... View More

NEFL Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)

NEFL Antibody in Immunohistochemistry (IHC)

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NEFL Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across tissue tested owing to their inherent genetic constitution. Relative expression of NEFL was observed in PC-12 differentiated to neurons in comparison to PC-12 undifferentiated cells. Relative expression was also observed in Mouse Cerebellum, Mouse Brain and Rat Brain in comparison to Mouse Heart and Mouse Spleen using Anti-NEFL Monoclonal Antibody (DA2) (Product # 13-0400) in Western Blot. {RE}

Product Details

13-0400

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5-2 µg/mL

View 10 publications 10 publications

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:20

View 3 publications 3 publications

ELISA (ELISA)

0.1-0.5 µg/mL

Immunoprecipitation (IP)

2-5 µg

Miscellaneous PubMed (Misc)

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

DA2

Immunogen

Specifically recognizes a phosphate-independent epitope on NF-L.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

purified

Storage buffer

PBS

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2532995

Product Specific Information

13-0400 detects the neurofilament, light chain in human, rat, and mouse samples.

Target Information

Involved in the maintenance of neuronal caliber, neurofilaments are the intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. NF-L is the light or low molecular weight microfilament subunit and runs on SDS-PAGE gels at approximately 70 kDa. Neurofilament are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-H is the heavy or high molecular weight microfilament subunit and runs on SDS-PAGE gels in the range 180-220 kDa, with some variation in different species.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Disrupted myelin lipid metabolism differentiates frontotemporal dementia caused by GRN and C9orf72 gene mutations.

Acta neuropathologica communications

Marian OC,Teo JD,Lee JY,Song H,Kwok JB,Landin-Romero R,Halliday G,Don AS

13-0400 was used in Western Blotting to determine how brain lipid metabolism is affected in familial FTD with TDP-43 inclusions, and how this is related to myelin and lysosomal markers.

Mon Mar 27 00:00:00 EDT 2023

Progenitor cells from brown adipose tissue undergo neurogenic differentiation.

Scientific reports

Jumabay M,Zhang L,Yao J,Boström KI

Published figure using NEFL monoclonal antibody (Product # 13-0400) in Immunocytochemistry

Mon Apr 04 00:00:00 EDT 2022

Progenitor cells from brown adipose tissue undergo neurogenic differentiation.

Scientific reports

Jumabay M,Zhang L,Yao J,Boström KI

Published figure using NEFL monoclonal antibody (Product # 13-0400) in Immunocytochemistry

Mon Apr 04 00:00:00 EDT 2022

Cep215 is essential for morphological differentiation of astrocytes.

Scientific reports

Kang D,Shin W,Yoo H,Kim S,Lee S,Rhee K

13-0400 was used in Western Blotting to show that Cep215 is involved in morphological differentiation of astrocytes. Cep215 was specifically localized at the glial processes as well as centrosomes in developing astrocytes.

Mon Oct 12 00:00:00 EDT 2020

Cep215 is essential for morphological differentiation of astrocytes.

Scientific reports

Kang D,Shin W,Yoo H,Kim S,Lee S,Rhee K

Mon Oct 12 00:00:00 EDT 2020

Impaired Neurofilament Integrity and Neuronal Morphology in Different Models of Focal Cerebral Ischemia and Human Stroke Tissue.

Frontiers in cellular neuroscience

Mages B,Aleithe S,Altmann S,Blietz A,Nitzsche B,Barthel H,Horn AKE,Hobusch C,Härtig W,Krueger M,Michalski D

13-0400 was used in Western Blotting to systematically address ischemia-induced alterations of the neurofilament light (NF-L), medium (NF-M), and heavy (NF-H) subunits as well as of α-internexin (INA).

Thu Oct 01 00:00:00 EDT 2020

Unbiased Proteomics of Early Lewy Body Formation Model Implicates Active Microtubule Affinity-Regulating Kinases (MARKs) in Synucleinopathies.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Henderson MX,Chung CH,Riddle DM,Zhang B,Gathagan RJ,Seeholzer SH,Trojanowski JQ,Lee VMY

13-0400 was used in Western Blotting to support a role for autophagy as a survival mechanism in response to stress due to ribosomal P proteins protein deficiency.

Wed Jun 14 00:00:00 EDT 2017

Substituting mouse transcription factor Pou4f2 with a sea urchin orthologue restores retinal ganglion cell development.

Proceedings. Biological sciences

Mao CA,Agca C,Mocko-Strand JA,Wang J,Ullrich-Lüter E,Pan P,Wang SW,Arnone MI,Frishman LJ,Klein WH

13-0400 was used in immunohistochemistry to characterize restoration of retinal ganglion cell development by substitution of mouse transcription factor Pou4f2 with a sea urchin orthologue

Wed Mar 16 00:00:00 EDT 2016

Muse Cells, a New Type of Pluripotent Stem Cell Derived from Human Fibroblasts.

Cellular reprogramming

Liu Q,Zhang RZ,Li D,Cheng S,Yang YH,Tian T,Pan XR

13-0400 was used in immunocytochemistry to characterize a new type of pluripotent stem cell derived from human fibroblasts called muse cells

Fri Apr 01 00:00:00 EDT 2016

Repeated Three-Hour Maternal Separation Induces Depression-Like Behavior and Affects the Expression of Hippocampal Plasticity-Related Proteins in C57BL/6N Mice.

Neural plasticity

Bian Y,Yang L,Wang Z,Wang Q,Zeng L,Xu G

13-0400 was used in western blot to elucidate the mechanisms by which maternal separation negatively affects mice

Mon Aug 29 00:00:00 EDT 2016

Repeated Three-Hour Maternal Separation Induces Depression-Like Behavior and Affects the Expression of Hippocampal Plasticity-Related Proteins in C57BL/6N Mice.

Neural plasticity

Bian Y,Yang L,Wang Z,Wang Q,Zeng L,Xu G

13-0400 was used in western blot to elucidate the mechanisms by which maternal separation negatively affects mice

Mon Aug 29 00:00:00 EDT 2016

Ginseng Total Saponins Reverse Corticosterone-Induced Changes in Depression-Like Behavior and Hippocampal Plasticity-Related Proteins by Interfering with GSK-3 β -CREB Signaling Pathway.

Evidence-based complementary and alternative medicine : eCAM

Chen L,Dai J,Wang Z,Zhang H,Huang Y,Zhao Y

13-0400 was used in western blot to investigate the antidepressant mechanisms of ginseng total saponins using the corticosterone-induced mouse depression model.

Thu Feb 13 00:00:00 EST 2014

Phagocytosis of neuronal debris by microglia is associated with neuronal damage in multiple sclerosis.

Glia

Huizinga R,van der Star BJ,Kipp M,Jong R,Gerritsen W,Clarner T,Puentes F,Dijkstra CD,van der Valk P,Amor S

13-0400 was used in immunohistochemistry to investigate phagocytosis of neuronal antigens in a multiple sclerosis model.

Thu Mar 01 00:00:00 EST 2012

Beneficial effects of benzodiazepine diazepam on chronic stress-induced impairment of hippocampal structural plasticity and depression-like behavior in mice.

Behavioural brain research

Zhao Y,Wang Z,Dai J,Chen L,Huang Y,Zhan Z

13-0400 was used in western blot to report the results from four preclinical experiments to determine the effects of benzodiazepines using a chronic unpredictable stress model.

Sat Mar 17 00:00:00 EDT 2012

Near complete loss of retinal ganglion cells in the math5/brn3b double knockout elicits severe reductions of other cell types during retinal development.

Developmental biology

Moshiri A,Gonzalez E,Tagawa K,Maeda H,Wang M,Frishman LJ,Wang SW

13-0400 was used in immunohistochemistry - frozen section to determine the contribution of retinal ganglion cells to mammalian retina development

Tue Apr 15 00:00:00 EDT 2008

Changes in NMDA receptor subunits and interacting PSD proteins in dorsolateral prefrontal and anterior cingulate cortex indicate abnormal regional expression in schizophrenia.

Molecular psychiatry

Kristiansen LV,Beneyto M,Haroutunian V,Meador-Woodruff JH

13-0400 was used in western blot to measure the expression of the NR1 subunit and the NR2A-D subunits in the dorsolateral prefrontal and anterior cingulate cortex in samples from schizophrenic patients and controls

Tue Aug 01 00:00:00 EDT 2006

A NUDEL-dependent mechanism of neurofilament assembly regulates the integrity of CNS neurons.

Nature cell biology

Nguyen MD,Shu T,Sanada K,Larivière RC,Tseng HC,Park SK,Julien JP,Tsai LH

Thu Jul 01 00:00:00 EDT 2004

A NUDEL-dependent mechanism of neurofilament assembly regulates the integrity of CNS neurons.

Nature cell biology

Nguyen MD,Shu T,Sanada K,Larivière RC,Tseng HC,Park SK,Julien JP,Tsai LH

Thu Jul 01 00:00:00 EDT 2004

A NUDEL-dependent mechanism of neurofilament assembly regulates the integrity of CNS neurons.

Nature cell biology

Nguyen MD,Shu T,Sanada K,Larivière RC,Tseng HC,Park SK,Julien JP,Tsai LH

Thu Jul 01 00:00:00 EDT 2004

A NUDEL-dependent mechanism of neurofilament assembly regulates the integrity of CNS neurons.

Nature cell biology

Nguyen MD,Shu T,Sanada K,Larivière RC,Tseng HC,Park SK,Julien JP,Tsai LH

Thu Jul 01 00:00:00 EDT 2004

Brn3b/Brn3c double knockout mice reveal an unsuspected role for Brn3c in retinal ganglion cell axon outgrowth.

Development (Cambridge, England)

Wang SW,Mu X,Bowers WJ,Kim DS,Plas DJ,Crair MC,Federoff HJ,Gan L,Klein WH

Tue Jan 01 00:00:00 EST 2002

The TSC1 tumor suppressor hamartin interacts with neurofilament-L and possibly functions as a novel integrator of the neuronal cytoskeleton.

The Journal of biological chemistry

Haddad LA,Smith N,Bowser M,Niida Y,Murthy V,Gonzalez-Agosti C,Ramesh V

Fri Nov 15 00:00:00 EST 2002

The TSC1 tumor suppressor hamartin interacts with neurofilament-L and possibly functions as a novel integrator of the neuronal cytoskeleton.

The Journal of biological chemistry

Haddad LA,Smith N,Bowser M,Niida Y,Murthy V,Gonzalez-Agosti C,Ramesh V

Fri Nov 15 00:00:00 EST 2002

Numerous conglomerate inclusions in slowly progressive familial amyotrophic lateral sclerosis with posterior column involvement.

Journal of the neurological sciences

Katayama S,Watanabe C,Noda K,Ohishi H,Yamamura Y,Nishisaka T,Inai K,Asayama K,Murayama S,Nakamura S

13-0400 was used in immunohistochemistry (paraffin) to characterize disease in a patient with familial amyotrophic lateral sclerosis.

Wed Dec 01 00:00:00 EST 1999

Bioinformatics

Protein Aliases: 68 kDa neurofilament protein; FLJ53642; light molecular weight neurofilament protein; NEF3; neurofilament; neurofilament 3; Neurofilament light polypeptide; neurofilament protein L; neurofilament protein, light chain; neurofilament subunit NF-L; Neurofilament triplet L protein; neurofilament, light polypeptide 68kDa; neurofilament-l; neurofilament-light; NF-L; NFM; NLC; OTTHUMP00000196614; protein phosphatase 1, regulatory subunit 110

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Gene Aliases: AI847934; CMT1F; CMT2E; NEFL; NF-L; NF68; NFL; PPP1R110

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UniProt ID: (Human) P07196, (Mouse) P08551, (Rat) P19527

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Entrez Gene ID: (Human) 4747, (Mouse) 18039, (Rat) 83613

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Function(s)

Ras guanyl-nucleotide exchange factor activity structural constituent of cytoskeleton protein binding protein C-terminus binding protein domain specific binding protein binding, bridging identical protein binding phospholipase binding protein heterodimerization activity structural molecule activity

Process(es)

MAPK cascade microtubule cytoskeleton organization anterograde axonal transport retrograde axonal transport response to toxic substance peripheral nervous system axon regeneration axonal transport of mitochondrion spinal cord development hippocampus development cerebral cortex development regulation of axon diameter neurofilament bundle assembly locomotion response to peptide hormone negative regulation of neuron apoptotic process positive regulation of GTPase activity intermediate filament polymerization or depolymerization intermediate filament organization neuron projection morphogenesis positive regulation of axonogenesis neuromuscular process controlling balance protein polymerization response to corticosterone response to sodium arsenite response to acrylamide intermediate filament bundle assembly neurofilament cytoskeleton organization response to organic substance response to organonitrogen compound

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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NEFL Monoclonal Antibody (DA2)

NEFL Antibody (13-0400) in IHC (P)

Product Details

13-0400

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Immunoprecipitation (IP)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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