Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
NEFL Monoclonal Antibody (F.852.7)
Advanced Verification
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 2
NEFL Antibody (MA5-15097) in WB
Western blot was performed using Anti-NEFL Monoclonal Antibody (F.852.7) (Product # MA5-15097) and a 68 kDa band corresponding to NEFL was observed in Mouse Cerebellum, Mouse Brain and Rat Brain but not in Mouse Heart and Mouse Testis. Tissue extracts (30 µg lysate) of Mouse Cerebellum (Lane 1), Mouse Brain (Lane 2), Rat Brain (Lane 3), Mouse Heart (Lane 4) and Mouse Testis (Lane 5) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Pr... View More
Product Details
MA5-15097
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Pig,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
F.852.7
Immunogen
Purified and enzymatically dephosphorylated pig neurofilament, light chain
Conjugate
Form
Liquid
Concentration
57 µg/mL
Purification
Affinity chromatography
Storage buffer
0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
Contains
<0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_10978383
Product Specific Information
It is not recommended to aliquot this antibody.
Target Information
Involved in the maintenance of neuronal caliber, neurofilaments are the intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. NF-L is the light or low molecular weight microfilament subunit and runs on SDS-PAGE gels at approximately 70 kDa. Neurofilament are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-H is the heavy or high molecular weight microfilament subunit and runs on SDS-PAGE gels in the range 180-220 kDa, with some variation in different species.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: 68 kDa neurofilament protein; FLJ53642; light molecular weight neurofilament protein; NEF3; neurofilament; neurofilament 3; Neurofilament light polypeptide; neurofilament protein L; neurofilament protein, light chain; neurofilament subunit NF-L; Neurofilament triplet L protein; neurofilament, light polypeptide; neurofilament, light polypeptide 68kDa; neurofilament-l; neurofilament-light; NF-L; NFM; NLC; OTTHUMP00000196614; protein phosphatase 1, regulatory subunit 110
Gene Aliases: AI847934; CMT1F; CMT2E; NEFL; NF-L; NF68; NFL; PPP1R110
UniProt ID: (Human) P07196, (Mouse) P08551, (Rat) P19527
Entrez Gene ID: (Human) 4747, (Pig) 100521224, (Mouse) 18039, (Rat) 83613
MAPK cascade
microtubule cytoskeleton organization
anterograde axonal transport
retrograde axonal transport
response to toxic substance
peripheral nervous system axon regeneration
axonal transport of mitochondrion
spinal cord development
hippocampus development
cerebral cortex development
regulation of axon diameter
neurofilament bundle assembly
locomotion
response to peptide hormone
negative regulation of neuron apoptotic process
positive regulation of GTPase activity
intermediate filament polymerization or depolymerization
intermediate filament organization
neuron projection morphogenesis
positive regulation of axonogenesis
neuromuscular process controlling balance
protein polymerization
response to corticosterone
response to sodium arsenite
response to acrylamide
axon development
intermediate filament bundle assembly
neurofilament cytoskeleton organization
response to organic substance
response to organonitrogen compound
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support