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Invitrogen
NEFM Polyclonal Antibody
Advanced Verification
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 6
NEFM Antibody (PA5-19200) in ICC/IF
Immunofluorescence analysis of NF-M was performed using 70% confluent log phase HEK-293 and HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. HEK-293 cells were labeled with NF-M Goat Polyclonal Antibody (Product # PA5-19200) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Rabbit anti-Goat IgG Heavy Chain Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conj... View More
Product Details
PA5-19200
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide sequence (QASHITVERKDYLK) corresponding to the internal amino acids of NEFM
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Ammonium sulfate precipitation
Storage buffer
TBS, pH 7.3, with 0.5% BSA
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_10983250
Product Specific Information
This antibody is predicted to react with bovine, canine, human, porcine and rat based on sequence homology.
This antibody is tested in Peptide ELISA: antibody detection limit dilution 128,000.
Target Information
Neurofilaments (NFs) are a type of intermediate filament (IF) expressed almost exclusively in neuronal cells, and in those cells most prominently in large axons. NFs, in most vertebrates, are composed of three different polypeptide chains with different molecular weights - neurofilament medium protein (NF-M), high (NF-H) and light protein (NF-L), which share sequence and structural similarity in a coiled-coil core domain, but differ in the length and sequence of their N-termini and more dramatically of their C-termini which in the case of NF-M and NF-H form the flexible extensions that link NFs to each other and to other elements in the cytoplasm. NF-M protein tail-mediated interactions of neurofilaments are critical for size and cytoskeletal architecture of axons, and are mediated, in part, by the highly phosphorylated tail domain of this protein. NF-M phosphorylation and O-GlcNAcylation are regulated reciprocally and affect its translocation and filament formation and function. Antibodies to the various neurofilament subunits are very useful cell type markers since the proteins are among the most abundant of the nervous system, are expressed only in neurons and are biochemically very stable.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 160 kDa neurofilament protein; Neurofilament 3; neurofilament 3, medium; Neurofilament medium polypeptide; neurofilament protein M; Neurofilament triplet M protein; neurofilament, medium polypeptide 150kDa; neurofilament-3 (150 kD medium); neurofilament-M; NF-M; NMC
Gene Aliases: NEF3; NEFM; NF-M; NF160; NF165; NFM
UniProt ID: (Human) P07197, (Mouse) P08553
Entrez Gene ID: (Human) 4741, (Mouse) 18040
neurofilament bundle assembly
axon development
mitophagy in response to mitochondrial depolarization
microtubule cytoskeleton organization
axo-dendritic transport
regulation of axon diameter
intermediate filament cytoskeleton organization
intermediate filament polymerization or depolymerization
intermediate filament bundle assembly
neurofilament cytoskeleton organization
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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