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Invitrogen
NEFM Polyclonal Antibody
Advanced Verification
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 1
NEFM Antibody (PA5-82815)
Relative expression in different tissues in IHC: Detection of differential expression levels of NEFM demonstrates antibody specificity. Immunohistochemical analysis of NEFM using anti-NEFM Polyclonal Antibody (Product #PA5-82815), shows significant staining of NEFM in cerebral cortex and shows minimal or weak staining in pancreas tissues. The relative expression levels of NEFM within each tissue is shown u... View More
Product Details
PA5-82815
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein corresponding to Human NEFM. Recombinant protein control fragment (Product #RP-93757).
Conjugate
Form
Liquid
Concentration
0.4 mg/mL
Purification
Anitgen affinity chromatography
Storage buffer
PBS, pH 7.2, with 40% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2789971
Product Specific Information
Immunogen sequence: YIEKVHYLEQ QNKEIEAEIQ ALRQKQASHA QLGDAYDQEI RELRATLEMV NHEKAQVQLD SDHLEEDIHR LKERFEEEAR LRDDTEAAIR ALRKDIEEAS LVKVELDKKV QSLQDEVAFL RSNH
Target Information
Neurofilaments (NFs) are a type of intermediate filament (IF) expressed almost exclusively in neuronal cells, and in those cells most prominently in large axons. NFs, in most vertebrates, are composed of three different polypeptide chains with different molecular weights - neurofilament medium protein (NF-M), high (NF-H) and light protein (NF-L), which share sequence and structural similarity in a coiled-coil core domain, but differ in the length and sequence of their N-termini and more dramatically of their C-termini which in the case of NF-M and NF-H form the flexible extensions that link NFs to each other and to other elements in the cytoplasm. NF-M protein tail-mediated interactions of neurofilaments are critical for size and cytoskeletal architecture of axons, and are mediated, in part, by the highly phosphorylated tail domain of this protein. NF-M phosphorylation and O-GlcNAcylation are regulated reciprocally and affect its translocation and filament formation and function. Antibodies to the various neurofilament subunits are very useful cell type markers since the proteins are among the most abundant of the nervous system, are expressed only in neurons and are biochemically very stable.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 160 kDa neurofilament protein; Neurofilament 3; neurofilament 3, medium; Neurofilament medium polypeptide; neurofilament protein M; Neurofilament triplet M protein; neurofilament, medium polypeptide 150kDa; neurofilament-3 (150 kD medium); neurofilament-M; NF-M; NMC
Gene Aliases: NEF3; NEFM; NF-M; NF160; NF165; NFM
UniProt ID: (Human) P07197, (Mouse) P08553
Entrez Gene ID: (Human) 4741, (Mouse) 18040
neurofilament bundle assembly
axon development
mitophagy in response to mitochondrial depolarization
microtubule cytoskeleton organization
axo-dendritic transport
regulation of axon diameter
intermediate filament cytoskeleton organization
intermediate filament polymerization or depolymerization
intermediate filament bundle assembly
neurofilament cytoskeleton organization
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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