Proteintech

NEIL3 Polyclonal Antibody

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NEIL3 Antibody (11621-1-AP) in WB

Mouse skin tissue were subjected to SDS PAGE followed by western blot with 11621-1-AP (NEIL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Product Details

11621-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 10 publications 10 publications

Flow Cytometry (Flow)

View 1 publication 1 publication

Immunoprecipitation (IP)

Assay-dependent

View 3 publications 3 publications

ChIP assay (ChIP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

NEIL3 Fusion Protein Ag2200 (257-605 aa encoded by BC025954)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.35 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: CHCRITVCR FGDNNRMTYF CPHCQKENPQ HVDICKLPTR NTIISWTSSR VDHVMDSVAR KSEEHWTCVV CTLINKPSSK ACDACLTSRP IDSVLKSEEN STVFSHLMKY PCNTFGKPHT EVKINRKTAF GTTTLVLTDF SNKSSTLERK TKQNQILDEE FQNSPPASVC LNDIQHPSKK TTNDITQLSS KVNISPTISS ESKLFSPAHK KPKTAHYSSP ELKSCNPGYS NSELQINMTD GPRTLNPDSP RCSKHNRLCI LRVVRKDGEN KGRQFYACPL PREAQCGFFE WADLSFPFCN HGKRSTMKTV LKIGPNNGKN FFVCPLGKEK QCNFFQWAEN GPGIKIIPGC (257-605 aa encoded by BC025954)

Target Information

DNA glycosylase which prefers single-stranded DNA (ssDNA), or partially ssDNA structures such as bubble and fork structures, to double-stranded DNA (dsDNA). In vitro, displays strong glycosylase activity towards the hydantoin lesions spiroiminodihydantoin (Sp) and guanidinohydantoin (Gh) in both ssDNA and dsDNA; also recognizes FapyA, FapyG, 5-OHU, 5-OHC, 5-OHMH, Tg and 8-oxoA lesions in ssDNA. No activity on 8-oxoG detected. Also shows weak DNA-(apurinic or apyrimidinic site) lyase activity. In vivo, appears to be the primary enzyme involved in removing Sp and Gh from ssDNA in neonatal tissues. Seems to be an important facilitator of cell proliferation in certain populations, for example neural stem/progenitor cells and tumor cells, suggesting a role in replication-associated DNA repair.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

SNHG3 regulates NEIL3 via transcription factor E2F1 to mediate malignant proliferation of hepatocellular carcinoma.

Immunogenetics

Zhang F,Lu J,Yang J,Dai Q,Du X,Xu Y,Zhang C

Wed Feb 01 00:00:00 EST 2023

NEIL3-mediated proteasomal degradation facilitates the repair of cisplatin-induced DNA damage in human cells.

Scientific reports

Aliyaskarova U,Baiken Y,Renaud F,Couve S,Kisselev AF,Saparbaev M,Groisman R

Thu Mar 30 00:00:00 EDT 2023

NEIL3 contributes to the Fanconi anemia/BRCA pathway by promoting the downstream double-strand break repair step.

Cell reports

Li N,Xu Y,Chen H,Chen L,Zhang Y,Yu T,Yao R,Chen J,Fu Q,Zhou J,Wang J

Tue Nov 08 00:00:00 EST 2022

NEIL3 contributes to the Fanconi anemia/BRCA pathway by promoting the downstream double-strand break repair step.

Cell reports

Li N,Xu Y,Chen H,Chen L,Zhang Y,Yu T,Yao R,Chen J,Fu Q,Zhou J,Wang J

Tue Nov 08 00:00:00 EST 2022

PrimPol-mediated repriming facilitates replication traverse of DNA interstrand crosslinks.

The EMBO journal

González-Acosta D,Blanco-Romero E,Ubieto-Capella P,Mutreja K,Míguez S,Llanos S,García F,Muñoz J,Blanco L,Lopes M,Méndez J

Thu Jul 15 00:00:00 EDT 2021

Cooperation of the NEIL3 and Fanconi anemia/BRCA pathways in interstrand crosslink repair.

Nucleic acids research

Li N,Wang J,Wallace SS,Chen J,Zhou J,D'Andrea AD

Mon Apr 06 00:00:00 EDT 2020

Oxidative DNA-protein crosslinks formed in mammalian cells by abasic site lyases involved in DNA repair.

DNA repair

Quiñones JL,Thapar U,Wilson SH,Ramsden DA,Demple B

Sun Mar 01 00:00:00 EST 2020

TRAIP is a master regulator of DNA interstrand crosslink repair.

Nature

Wu RA,Semlow DR,Kamimae-Lanning AN,Kochenova OV,Chistol G,Hodskinson MR,Amunugama R,Sparks JL,Wang M,Deng L,Mimoso CA,Low E,Patel KJ,Walter JC

Fri Mar 01 00:00:00 EST 2019

C/EBPβ enhances platinum resistance of ovarian cancer cells by reprogramming H3K79 methylation.

Nature communications

Liu D,Zhang XX,Li MC,Cao CH,Wan DY,Xi BX,Tan JH,Wang J,Yang ZY,Feng XX,Ye F,Chen G,Wu P,Xi L,Wang H,Zhou JF,Feng ZH,Ma D,Gao QL

Mon Apr 30 00:00:00 EDT 2018

C/EBPβ enhances platinum resistance of ovarian cancer cells by reprogramming H3K79 methylation.

Nature communications

Liu D,Zhang XX,Li MC,Cao CH,Wan DY,Xi BX,Tan JH,Wang J,Yang ZY,Feng XX,Ye F,Chen G,Wu P,Xi L,Wang H,Zhou JF,Feng ZH,Ma D,Gao QL

Mon Apr 30 00:00:00 EDT 2018

NEIL3 Repairs Telomere Damage during S Phase to Secure Chromosome Segregation at Mitosis.

Cell reports

Zhou J,Chan J,Lambelé M,Yusufzai T,Stumpff J,Opresko PL,Thali M,Wallace SS

Tue Aug 29 00:00:00 EDT 2017

NEIL3 Repairs Telomere Damage during S Phase to Secure Chromosome Segregation at Mitosis.

Cell reports

Zhou J,Chan J,Lambelé M,Yusufzai T,Stumpff J,Opresko PL,Thali M,Wallace SS

Tue Aug 29 00:00:00 EDT 2017

NEIL3 Repairs Telomere Damage during S Phase to Secure Chromosome Segregation at Mitosis.

Cell reports

Zhou J,Chan J,Lambelé M,Yusufzai T,Stumpff J,Opresko PL,Thali M,Wallace SS

Tue Aug 29 00:00:00 EDT 2017

NEIL3 Repairs Telomere Damage during S Phase to Secure Chromosome Segregation at Mitosis.

Cell reports

Zhou J,Chan J,Lambelé M,Yusufzai T,Stumpff J,Opresko PL,Thali M,Wallace SS

Tue Aug 29 00:00:00 EDT 2017

Release from quiescence stimulates the expression of human NEIL3 under the control of the Ras dependent ERK-MAP kinase pathway.

DNA repair

Neurauter CG,Luna L,Bjørås M

Sun Apr 01 00:00:00 EDT 2012

Release from quiescence stimulates the expression of human NEIL3 under the control of the Ras dependent ERK-MAP kinase pathway.

DNA repair

Neurauter CG,Luna L,Bjørås M

Sun Apr 01 00:00:00 EDT 2012

Release from quiescence stimulates the expression of human NEIL3 under the control of the Ras dependent ERK-MAP kinase pathway.

DNA repair

Neurauter CG,Luna L,Bjørås M

Sun Apr 01 00:00:00 EDT 2012

Release from quiescence stimulates the expression of human NEIL3 under the control of the Ras dependent ERK-MAP kinase pathway.

DNA repair

Neurauter CG,Luna L,Bjørås M

Sun Apr 01 00:00:00 EDT 2012

Bioinformatics

Protein Aliases: DNA glycosylase FPG2; DNA glycosylase hFPG2; DNA glycosylase/AP lyase Neil3; Endonuclease 8-like 3; Endonuclease VIII-like 3; FLJ10858; nei endonuclease VIII-like 3; nei-like DNA glycosylase 3; Nei-like protein 3; zinc finger, GRF-type containing 3

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Gene Aliases: AI449477; BC034753; C85903; FGP2; FPG2; hFPG2; hNEI3; NEI3; NEIL3; ZGRF3

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UniProt ID: (Human) Q8TAT5, (Mouse) Q4ADY6

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Entrez Gene ID: (Human) 55247, (Mouse) 234258

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Function(s)

bubble DNA binding damaged DNA binding double-stranded DNA binding single-stranded DNA binding DNA-(apurinic or apyrimidinic site) lyase activity zinc ion binding DNA N-glycosylase activity purine-specific mismatch base pair DNA N-glycosylase activity nucleic acid binding DNA binding catalytic activity uracil DNA N-glycosylase activity hydrolase activity hydrolase activity, acting on glycosyl bonds hydrolase activity, hydrolyzing N-glycosyl compounds lyase activity single-strand selective uracil DNA N-glycosylase activity DNA-3-methylbase glycosylase activity metal ion binding nicotinamide riboside hydrolase activity nicotinic acid riboside hydrolase activity deoxyribonucleoside 5'-monophosphate N-glycosidase activity DNA glycosylase

Process(es)

base-excision repair nucleotide-excision repair DNA metabolic process DNA repair cellular response to DNA damage stimulus metabolic process nucleic acid phosphodiester bond hydrolysis

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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NEIL3 Polyclonal Antibody

NEIL3 Antibody (11621-1-AP) in WB

Product Details

11621-1-AP

Western Blot (WB)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

ChIP assay (ChIP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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