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NEK9 Antibody (PA5-115636) in ICC/IF
Immunocytochemistry analysis of NEK9 in HeLa cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-115636) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with mouse anti-beta tubulin; AlexaFluor 594 conjugated goat anti-rabbit IgG (Red); AlexaFluor 88 conjugated goat anti-mouse IgG (Green) and DAPI (blue) using a dilution of 1:200 (1 hr at 37°C).
Product Details
PA5-115636
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human NEK9(Accession Q8TD19), corresponding to amino acid residues S750-N800.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2900271
Product Specific Information
Antibody detects endogenous levels of total Nek9.
Target Information
NEK9, a NEK type protein kinase, regulates chromosome alignment and segregation in mitosis. The protein has a N-terminal NIMA-like catalytic domain, a central domain with homology to the guanine nucleotide exchange factor for the GTPase Ran (RCC1), and a C-terminal coiled-coil domain. It is phosphorylated by active p34(Cdc2) and is capable of autophosphorylation and oligomerization. The activated protein binds Nek6 and RCC1, and has been shown to associate with the human homolog of the Drosophila protein Bicaudal D (Bicd2) in vivo and to phosphorylate this coiled-coil protein in vitro. NEK9 expression has been documented in normal human brain. ESTs have been isolated from several tissue libraries, including normal bone, lymph and thymus and cancerous cervix, eye, lymph and placenta.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DKFZp434D0935; MGC138306; MGC16714; NEK 9; Nek8; Nercc 1 kinase; Nercc1 kinase; Never in mitosis A-related kinase 9; NIMA (never in mitosis gene a)- related kinase 9; NimA-related kinase 8; NimA-related protein kinase 9; Serine/threonine-protein kinase Nek9
Gene Aliases: APUG; C130021H08Rik; KIAA1995; LCCS10; NC; NEK8; NEK9; NERCC; NERCC1
UniProt ID: (Human) Q8TD19, (Mouse) Q8K1R7
Entrez Gene ID: (Human) 91754, (Mouse) 217718
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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