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NF-H Antibody (MA5-47470) in ICC/IF
Immunocytochemistry analysis of NF-H in E20 rat cortex grown in culture. Samples were stained with NF-H monoclonal antibody (Product # MA5-47470) using a dilution of 1:5,000 (red). Costained with polyclonal antibody to neurofilament NF-L, dilution 1:5,000 in green. The NF-H antibody binds to phosphorylated NF-H found in some mature axonal profiles, while the NF-L binds to both axonal, dendritic and developing neuronal processes. The mature axonal profiles contain both NF-L and phosphorylated NF-H and so appear yellow, while processes expre... View More
Product Details
MA5-47470
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
9B12
Immunogen
Native NF-H purified from bovine spinal cord, binding to phosphorylated KSP sequences
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 50% glycerol
Contains
5mM sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2942452
Target Information
Involved in the maintenance of neuronal caliber, neurofilaments are the intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. NF-H has an important role in mature axons and is involved in amyotrophic lateral sclerosis (ALS).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 200 kDa neurofilament protein; NEF3; neurofilament; neurofilament 200kDa; Neurofilament heavy polypeptide; Neurofilament triplet H protein; neurofilament, heavy polypeptide 200kDa; NF 200 kD; NF-H; NHC
Gene Aliases: CMT2CC; KIAA0845; mKIAA0845; NEFH; NF-H; NF200; NFH
UniProt ID: (Human) P12036, (Mouse) P19246
Entrez Gene ID: (Human) 4744, (Rat) 24587, (Mouse) 380684
microtubule cytoskeleton organization
ovarian follicle atresia
axonogenesis
spinal cord development
hippocampus development
cerebral cortex development
cell projection assembly
axon regeneration
neurofilament bundle assembly
cellular response to oxidative stress
response to cocaine
peripheral nervous system neuron axonogenesis
axon development
cellular response to estradiol stimulus
regulation of organelle transport along microtubule
response to sodium arsenite
response to acrylamide
brain development
embryo development
neuron projection development
intermediate filament cytoskeleton organization
intermediate filament bundle assembly
neurofilament cytoskeleton organization
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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