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Invitrogen
NF-H Monoclonal Antibody (RNF 402)
Advanced Verification
This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 3
NF-H Antibody (OMA1-06116) in ICC/IF
Knockout of NF-H was achieved by CRISPR-Cas9 genome editing using LentiArray™ Lentiviral sgRNA (Product # A32042, AssayID CRISPR819012_LV) and LentiArray Cas9 Lentivirus (Product # A32064). Immunofluorescence analysis was performed on wild type SH-SY5Y cells (panel a,d), SH-SY5Y Cas9 control cells (panel b,e) and SH-SY5Y NF-HKO cells (panel c, f). Cells were fixed, permeabilized, and labeled with NF-H Monoclonal Antibody (RNF 402) (Product # OMA1-06116 , 1:100), followed by Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody... View More
Product Details
OMA1-06116
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Dog,
Chicken,
Guinea pig,
Hamster,
Human,
Mouse,
Non-human primate,
Sheep,
Rabbit,
Rat,
Xenopus
Host/Isotype
Mouse
/ IgM
Class
Monoclonal
Type
Antibody
Clone
RNF 402
Immunogen
Neurofilament preparation of calf brain tissue
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Ammonium sulfate precipitation
Storage buffer
PBS
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_325537
Product Specific Information
OMA1-06116 detects both the phosphorylated and non-phosphorylated forms of the 200 kDa neurofilament from rat, guinea pig, rabbit, dog, hamster, chicken, mouse, cow, monkey, sheep, xenopus and human tissues.
OMA1-06116 has been successfully used in immunohistochemical (frozen & paraffin) and Western blot procedures. By Western blot, this antibody detects a single ~200 kDa protein representing both forms of the 200 kDa neurofilament from rat brain homogenate.
OMA1-06116 antigen is cytoskeletal preparation of calf brain tissue.
Target Information
Involved in the maintenance of neuronal caliber, neurofilaments are the intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. NF-H has an important role in mature axons and is involved in amyotrophic lateral sclerosis (ALS).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 200 kDa neurofilament protein; heavy neurofilament protein; intermediate filament protein; NEF3; neurofilament; neurofilament 200kDa; Neurofilament heavy polypeptide; Neurofilament triplet H protein; neurofilament, heavy polypeptide; neurofilament, heavy polypeptide 200kDa; neurofilament, heavy polypeptide L homeolog; neurofilament-H; NF 200 kD; NF-H; NHC
Gene Aliases: CMT2CC; I79_012512; KIAA0845; mKIAA0845; NEFH; NF-H; NF200; NFH; XELAEV_18007418mg
UniProt ID: (Human) P12036, (Mouse) P19246
microtubule cytoskeleton organization
ovarian follicle atresia
axonogenesis
spinal cord development
hippocampus development
cerebral cortex development
cell projection assembly
axon regeneration
neurofilament bundle assembly
cellular response to oxidative stress
response to cocaine
peripheral nervous system neuron axonogenesis
axon development
cellular response to estradiol stimulus
regulation of organelle transport along microtubule
response to sodium arsenite
response to acrylamide
brain development
embryo development
neuron projection development
intermediate filament cytoskeleton organization
intermediate filament bundle assembly
neurofilament cytoskeleton organization
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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