Proteintech

NF-L Polyclonal Antibody

NF-L Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 12

NF-L Antibody (12998-1-AP) in ICC/IF

Immunofluorescent analysis of SH-SY5Y cells using 12998-1-AP (NF-L antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

NF-L Antibody in Immunohistochemistry (Paraffin) (IHC (P))

NF-L Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

12998-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:4,000

View 10 publications 10 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:400

Immunocytochemistry (ICC/IF)

1:20-1:200

View 6 publications 6 publications

Flow Cytometry (Flow)

Assay-dependent

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Not applicable (N/A)

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

NF-L Fusion Protein Ag3667 (1-352 aa encoded by BC039237)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.43 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

This antibody is specific to NEFL. It has a very weak reaction to NEFM.

Immunogen sequence: MSSFSYEPY YSTSYKRRYV ETPRVHISSV RSGYSTARSA YSSYSAPVSS SLSVRRSYSS SSGSLMPSLE NLDLSQVAAI SNDLKSIRTQ EKAQLQDLND RFASFIERVH ELEQQNKVLE AELLVLRQKH SEPSRFRALY EQEIRDLRLA AEDATNEKQA LQGEREGLEE TLRNLQARYE EEVLSREDAE GRLMEARKGA DEAALARAEL EKRIDSLMDE ISFLKKVHEE EIAELQAQIQ YAQISVEMDV TKPDLSAALK DIRAQYEKLA AKNMQNAEEW FKSRFTVLTE SAAKNTDAVR AAKDEVSESR RLLKAKTLEI EACRGMNEAL EKQLQELEDK QNADISAMQD TIN (1-352 aa encoded by BC039237)

Target Information

Involved in the maintenance of neuronal caliber, neurofilaments are the intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. NF-L is the light or low molecular weight microfilament subunit and runs on SDS-PAGE gels at approximately 70 kDa. Neurofilament are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-H is the heavy or high molecular weight microfilament subunit and runs on SDS-PAGE gels in the range 180-220 kDa, with some variation in different species.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

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Sat Apr 01 00:00:00 EDT 2023

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Mon Nov 07 00:00:00 EST 2022

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Liu X,Wang J,Wang P,Zhong L,Wang S,Feng Q,Wei X,Zhou L

Wed Oct 19 00:00:00 EDT 2022

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A comprehensive mouse brain acetylome-the cellular-specific distribution of acetylated brain proteins.

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Ji Y,Chen Z,Cen Z,Ye Y,Li S,Lu X,Shao Q,Wang D,Ji J,Ji Q

Sat Sep 17 00:00:00 EDT 2022

A comprehensive mouse brain acetylome-the cellular-specific distribution of acetylated brain proteins.

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Perinatal exposure to nonylphenol delayed myelination in offspring cerebellum.

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Serum Protein Biomarker Findings Reflective of Oxidative Stress and Vascular Abnormalities in Male, but Not Female, Collision Sport Athletes.

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Advanced science (Weinheim, Baden-Wurttemberg, Germany)

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Sun Oct 01 00:00:00 EDT 2017

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Bioinformatics

Protein Aliases: 68 kDa neurofilament protein; FLJ53642; light molecular weight neurofilament protein; NEF3; neurofilament; neurofilament 3; Neurofilament light polypeptide; neurofilament protein L; neurofilament protein, light chain; neurofilament subunit NF-L; Neurofilament triplet L protein; neurofilament, light polypeptide 68kDa; neurofilament-l; neurofilament-light; NF-L; NFM; NLC; OTTHUMP00000196614; protein phosphatase 1, regulatory subunit 110

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Gene Aliases: AI847934; CMT1F; CMT2E; NEFL; NF-L; NF68; NFL; PPP1R110

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UniProt ID: (Human) P07196, (Mouse) P08551, (Rat) P19527

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Entrez Gene ID: (Human) 4747, (Mouse) 18039, (Rat) 83613

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Function(s)

Ras guanyl-nucleotide exchange factor activity structural constituent of cytoskeleton protein binding protein C-terminus binding protein domain specific binding protein binding, bridging identical protein binding phospholipase binding protein heterodimerization activity structural molecule activity

Process(es)

MAPK cascade microtubule cytoskeleton organization anterograde axonal transport retrograde axonal transport response to toxic substance peripheral nervous system axon regeneration axonal transport of mitochondrion spinal cord development hippocampus development cerebral cortex development regulation of axon diameter neurofilament bundle assembly locomotion response to peptide hormone negative regulation of neuron apoptotic process positive regulation of GTPase activity intermediate filament polymerization or depolymerization intermediate filament organization neuron projection morphogenesis positive regulation of axonogenesis neuromuscular process controlling balance protein polymerization response to corticosterone response to sodium arsenite response to acrylamide intermediate filament bundle assembly neurofilament cytoskeleton organization response to organic substance response to organonitrogen compound

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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NF-L Polyclonal Antibody

NF-L Antibody (12998-1-AP) in ICC/IF

Product Details

12998-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Miscellaneous PubMed (Misc)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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