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NF2 Antibody (PA5-96130) in WB
Western blot analysis of NF2 in extracts of various cell lines. Samples were incubated with NF2 Polyclonal antibody (Product # PA5-96130) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
Product Details
PA5-96130
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 19-595 of human NF2 (NP_0002591)
Conjugate
Form
Liquid
Concentration
0.68 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.05% ProClin 300
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2807932
Product Specific Information
Positive Samples: HeLa, 293T, HepG2; Cellular Location: Cell projection, Cytoplasm, Cytoplasmic granule, Cytoplasmic side, Nucleus, Nucleus, Peripheral membrane protein, cytoskeleton, filopodium membrane, perinuclear region, ruffle membrane
Immunogen sequence: PKTFTVRIVT MDAEMEFNCE MKWKGKDLFD LVCRTLGLRE TWFFGLQYTI KDTVAWLKMD KKVLDHDVSK EEPVTFHFLA KFYPENAEEE LVQEITQHLF FLQVKKQILD EKIYCPPEAS VLLASYAVQA KYGDYDPSVH KRGFLAQEEL LPKRVINLYQ MTPEMWEERI TAWYAEHRGR ARDEAEMEYL KIAQDLEMYG VNYFAIRNKK GTELLLGVDA LGLHIYDPEN RLTPKISFPW NEIRNISYSD KEFTIKPLDK KIDVFKFNSS KLRVNKLILQ LCIGNHDLFM RRRKADSLEV QQMKAQAREE KARKQMERQR LAREKQMREE AERTRDELER RLLQMKEEAT MANEALMRSE ETADLLAEKA QITEEEAKLL AQKAAEAEQE MQRIKATAIR TEEEKRLMEQ KVLEAEVLAL KMAEESERRA KEADQLKQDL QEAREAERRA KQKLLEIATK PTYPPMNPIP APLPPDIPSF NLIGDSLSFD FKDTDMKRLS MEIEKEKVEY MEKSKHLQEQ LNELKTEIEA LKLKERETAL DILHNENSDR GGSSKHNTIK KLTLQSAKSR VAFFEEL
Target Information
NF2 encodes a protein, Merlin, that is similar to some members of the ERM family of proteins that are thought to link cytoskeletal components with proteins in the cell membrane. Merlin has been shown to interact with cell-surface proteins, proteins involved in cytoskeletal dynamics and proteins involved in regulating ion transport. This gene is expressed at high levels during embryonic development; in adults, significant expression is found in Schwann cells, meningeal cells, lens and nerve. Mutations in this gene are associated with neurofibromatosis type II which is characterized by nervous system and skin tumors and ocular abnormalities. Two predominant isoforms and a number of minor isoforms are produced by alternatively spliced transcripts.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Merlin; MGC123124; moesin-ezrin-radixin like; Moesin-ezrin-radixin-like protein; moesin-ezrin-radizin-like protein; neurofibromatosis 2; neurofibromin 2 (bilateral acoustic neuroma); neurofibromin 2 (merlin); Neurofibromin-2; OTTHUMP00000199607; Schwannomerlin; Schwannomin
Gene Aliases: ACN; BANF; merlin; Nf-2; NF2; SCH
UniProt ID: (Human) P35240, (Mouse) P46662, (Rat) Q63648
Entrez Gene ID: (Human) 4771, (Mouse) 18016, (Rat) 25744
mesoderm formation
negative regulation of cell-matrix adhesion
negative regulation of protein kinase activity
ectoderm development
negative regulation of DNA replication
negative regulation of cell proliferation
Schwann cell proliferation
regulation of gliogenesis
hippocampus development
negative regulation of cell-cell adhesion
actin cytoskeleton organization
negative regulation of cell growth
negative regulation of cell migration
regulation of protein stability
regulation of hippo signaling
odontogenesis of dentin-containing tooth
negative regulation of tyrosine phosphorylation of Stat3 protein
negative regulation of tyrosine phosphorylation of Stat5 protein
negative regulation of MAPK cascade
cell-cell junction organization
positive regulation of cell differentiation
negative regulation of JAK-STAT cascade
positive regulation of stress fiber assembly
lens fiber cell differentiation
regulation of stem cell proliferation
regulation of protein localization to nucleus
regulation of neural precursor cell proliferation
brain development
regulation of cell proliferation
regulation of neurogenesis
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