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NFAT5 Antibody (A305-174A) in WB
Detection of human NFAT5 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-NFAT5 antibody A305-174A (lot A305-174A-1) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Product Details
A305-174A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 1481 to 1531 of human Nuclear factor of activated T-cells 5.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
phosphate/tris citrate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4° C
Shipping conditions
Wet ice
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Based on 100% sequence identity, this antibody is predicted to react with Mouse and Rat
Target Information
The nuclear factor of activated T-cells (NFAT) transcription complex is required for the expression of a group of proteins that collectively regulate the immune response. Four NFAT proteins, encoded on separate genes and expressed as several splice variants, have been described: NFAT1 (also known as NFATp or NFATc2), NFAT2 (NFATc or NFATc1), NFAT3, and NFAT4 (NFATx or NFATc3). These proteins show a low level of sequence similarity with the Dorsal/Rel/NFkB family of transcription factors. Another NFAT-related protein termed NFAT5 differs from isoforms 1-4 in that it lacks many of the Fos/Jun contact sites observed in its predecessors and its subcellular localization is not calcineurin-dependent.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: glutamine rich protein H65; NF-AT5; NFAT-like protein 1; Nuclear factor of activated T-cells 5; osmotic response element-binding protein; T-cell transcription factor NFAT5; TonE-binding protein; Tonicity-responsive enhancer-binding protein
Gene Aliases: KIAA0827; NF-AT5; NFAT5; NFATL1; NFATZ; OREBP; TONEBP
UniProt ID: (Human) O94916
Entrez Gene ID: (Human) 10725
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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