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NFATC1 Antibody (PA5-79730) in WB
Western blot analysis of NFATC1 in Lane 1: mouse thymus tissue lysate, Lane 2: human 22RV1 whole cell lysate using 50 µg (reducing conditions) per well. Electrophoresis was performed on 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours and protein was transferred to a nitrocellulose membrane at 150mA for 50-90 minutes. Sample was blocked with 5% Non-fat Milk/TBS for 1.5 hours at room temperature, incubated with NFATC1 polyclonal antibody (Product # PA5-79730) at a dilution of 0.5 µg/mL (overnight at 4°C), followe... View More
Product Details
PA5-79730
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse,
Rat,
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived human NFAT2 recombinant protein (Position: Q589-K652).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2746845
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Target Information
The product of this gene is a component of the nuclear factor of activated T cells DNA-binding transcription complex. This complex consists of at least two components: a preexisting cytosolic component that translocates to the nucleus upon T cell receptor (TCR) stimulation, and an inducible nuclear component. Proteins belonging to this family of transcription factors play a central role in inducible gene transcription during immune response. The product of this gene is an inducible nuclear component. It functions as a major molecular target for the immunosuppressive drugs such as cyclosporin A. Five transcript variants encoding distinct isoforms have been identified for this gene. Different isoforms of this protein may regulate inducible expression of different cytokine genes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: MGC138448; NF-ATc; NF-ATc1; NFAT tra; NFAT trans; NFAT transcription complex cytosolic component; nuclear factor of activated T-cells 'c'; Nuclear factor of activated T-cells, cytoplasmic 1; nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 1; transcription factor NF-ATc
Gene Aliases: 2210017P03Rik; AI449492; AV076380; NF-ATC; NF-ATc1.2; NFAT2; NFATC; NFATC1; Nfatcb
UniProt ID: (Human) O95644
Entrez Gene ID: (Human) 4772, (Rat) 100361818, (Mouse) 18018
RNA polymerase II distal enhancer sequence-specific DNA binding
RNA polymerase II transcription factor binding
transcriptional activator activity, RNA polymerase II distal enhancer sequence-specific binding
transcription factor activity, sequence-specific DNA binding
transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding
protein binding
FK506 binding
mitogen-activated protein kinase p38 binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II core promoter sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
RNA polymerase II transcription coactivator binding
transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
chromatin binding
transcription factor binding
sequence-specific DNA binding
transcription regulatory region DNA binding
Rel homology transcription factor
transcription from RNA polymerase II promoter
Wnt signaling pathway, calcium modulating pathway
negative regulation of Wnt signaling pathway
calcineurin-NFAT signaling cascade
intracellular signal transduction
Fc-epsilon receptor signaling pathway
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
G1/S transition of mitotic cell cycle
negative regulation of transcription from RNA polymerase II promoter
epithelial to mesenchymal transition
lymphangiogenesis
B-1a B cell differentiation
heart valve morphogenesis
aortic valve morphogenesis
pulmonary valve morphogenesis
regulation of transcription from RNA polymerase II promoter
calcium ion transport
heart development
positive regulation of gene expression
transition between fast and slow fiber
osteoclast differentiation
regulation of hair cycle
negative regulation of osteoblast differentiation
ventricular septum morphogenesis
patterning of lymph vessels
heart trabecula morphogenesis
negative regulation of stem cell proliferation
transcription, DNA-templated
regulation of transcription, DNA-templated
cell quiescence
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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