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NFATC4 Antibody (PA1-021) in ICC/IF
Immunofluorescent analysis of NFAT3 using anti-NFAT3 polyclonal antibody (Product # PA1-021) (shown in green) in HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were then blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with a rabbit polyclonal antibody recognizing NFAT3 (Product # PA1-021), at a dilution of 1:100 for at least 1 hour at room temperature. Cells were washed with PBS and incubated with DyLight 488 goa... View More
Product Details
PA1-021
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to residues C R(887) D L S G F P A P P G E E P P A(902) of human NFAT3.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2267268
Product Specific Information
PA1-021 detects nuclear factor of activated T-cells 3 (NFAT3) from human cells.
PA1-021 has been successfully used in Western blot immunohistochemistry, immunofluorescence, and immunoprecipitation procedures. By Western blot, this antibody detects an ~150 kDa protein representing NFAT3 in HEK293 cells transfected with the human NFAT3 gene. Immunohistochemical staining of NFAT3 in HEK293 cells transfected with the human NFAT3 gene results in cytoplasmic staining.
The PA1-021 immunogen is a synthetic peptide corresponding to residues C R(887) D L S G F P A P P G E E P P A(902) of human NFAT3. This sequence is 94% conserved with mouse NFAT3. The PA1-021 immunizing peptide (Cat. # PEP-122) is available for use in neutralization and control experiments.
Target Information
This protein is an adapter or scaffold protein, which participates in the assembly of numerous protein complexes and is involved in several cellular processes such as cell fate determination, cytoskeletal organization, repression of gene transcription, mitosis, cell-cell adhesion, cell differentiation, proliferation and migration.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: NF-AT3; NF-ATc4; Nuclear factor of activated T-cells, cytoplasmic 4; nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 4; T-cell transcription factor NFAT3
Gene Aliases: NF-AT3; NF-ATC4; NFAT3; NFATC4
UniProt ID: (Human) Q14934
Entrez Gene ID: (Human) 4776
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding
transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding
transcription coactivator activity
protein binding
transcription factor binding
peroxisome proliferator activated receptor binding
Rel homology transcription factor
patterning of blood vessels
transcription from RNA polymerase II promoter
inflammatory response
heart development
intrinsic apoptotic signaling pathway in response to DNA damage
negative regulation of Wnt signaling pathway
negative regulation of protein binding
positive regulation of tumor necrosis factor production
calcineurin-NFAT signaling cascade
cellular response to UV
negative regulation of chromatin binding
cellular respiration
positive regulation of transcription from RNA polymerase II promoter
regulation of synaptic plasticity
negative regulation of dendrite morphogenesis
smooth muscle cell differentiation
muscle cell development
cellular response to lithium ion
negative regulation of pri-miRNA transcription from RNA polymerase II promoter
negative regulation of synapse maturation
positive regulation of apoptotic signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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