Proteintech

NFKB2 Polyclonal Antibody

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Antibody Testing Data (8)

NFKB2 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 8

NFKB2 Antibody (10409-2-AP) in ICC/IF

Immunofluorescent analysis of HeLa cells using 10409-2-AP (NFKB2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

NFKB2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

NFKB2 Antibody in Immunoprecipitation (IP)

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Product Details

10409-2-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 12 publications 12 publications

Immunohistochemistry (IHC)

View 6 publications 6 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:10-1:100

View 1 publication 1 publication

Immunoprecipitation (IP)

0.5-4.0 µg

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

NFKB2 Fusion Protein Ag0662 (600-899 aa encoded by BC002844)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.31 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human NFKB2.

Immunogen sequence: GLYPVHLAV RARSPECLDL LVDSGAEVEA TERQGGRTAL HLATEMEELG LVTHLVTKLR ANVNARTFAG NTPLHLAAGL GYPTLTRLLL KAGADIHAEN EEPLCPLPSP PTSDSDSDSE GPEKDTRSSF RGHTPLDLTC STKVKTLLLN AAQNTMEPPL TPPSPAGPGL SLGDTALQNL EQLLDGPEAQ GSWAELAERL GLRSLVDTYR QTTSPSGSLL RSYELAGGDL AGLLEALSDM GLEEGVRLLR GPETRDKLPS TEVKEDSAYG SQSVEQEAEK LGPPPEPPGG LCHGHPQPQV H (600-899 aa encoded by BC002844)

Target Information

The NFkB transcription factor was originally identified as a protein complex consisting of a DNA binding subunit and an associated protein. The subunit is functionally related to c-Rel p75 and Rel B p68. The p50 subunit was initially believed to be a functionally unique protein derived from the amino-terminus of a precursor designated p105. A cDNA has been isolated that encodes an alternative DNA binding subunit of NFkB. It is synthesized as a protein that is expressed in a variety of cell types and, like p105, undergoes cleavage to generate its NFkB subunit, in this case a protein designated p52 (previously referred to as p49). In contrast to p50 derived from p105, p52 acts in synergy with p65 to stimulate the HIV enhancer in transiently transfected Jurkat cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Transcription factor Dmrt1 triggers the SPRY1-NF-κB pathway to maintain testicular immune homeostasis and male fertility.

Zoological research

Zhang MF,Wan SC,Chen WB,Yang DH,Liu WQ,Li BL,Aierken A,Du XM,Li YX,Wu WP,Yang XC,Wei YD,Li N,Peng S,Li XL,Li GP,Hua JL

Thu May 18 00:00:00 EDT 2023

Changes Within H3K4me3-Marked Histone Reveal Molecular Background of Neutrophil Functional Plasticity.

Frontiers in immunology

Piatek P,Namiecinska M,Lewkowicz N,Kulińska-Michalska M,Jabłonowski Z,Matysiak M,Dulska J,Michlewska S,Wieczorek M,Lewkowicz P

Tue Jun 28 00:00:00 EDT 2022

BMC medical genomics

Wang M,Li Y,Yang J,Wang X,Zhang L

Fri Nov 25 00:00:00 EST 2022

TiO₂ nanosheets promote the transformation of vascular smooth muscle cells into foam cells in vitro and in vivo through the up-regulation of nuclear factor kappa B subunit 2.

Journal of hazardous materials

Li S,Yan D,Huang C,Yang F,Cao Y

Tue Feb 15 00:00:00 EST 2022

NFKB2 inhibits NRG1 transcription to affect nucleus pulposus cell degeneration and inflammation in intervertebral disc degeneration.

Mechanisms of ageing and development

Wang X,Wu H,Zhang Q,Liu F,Wang J,Wei C,Liu F,Li J

Thu Jul 01 00:00:00 EDT 2021

NFKB2 inhibits NRG1 transcription to affect nucleus pulposus cell degeneration and inflammation in intervertebral disc degeneration.

Mechanisms of ageing and development

Wang X,Wu H,Zhang Q,Liu F,Wang J,Wei C,Liu F,Li J

Thu Jul 01 00:00:00 EDT 2021

The possible protective effect of Nano-Selenium on the endocrine and exocrine pancreatic functions in a rat model of acute pancreatitis.

Journal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements (GMS)

Abdel-Hakeem EA,Abdel-Hamid HA,Abdel Hafez SMN

Wed Jul 01 00:00:00 EDT 2020

A translational program that suppresses metabolism to shield the genome.

Nature communications

Balukoff NC,Ho JJD,Theodoridis PR,Wang M,Bokros M,Llanio LM,Krieger JR,Schatz JH,Lee S

Fri Nov 13 00:00:00 EST 2020

circPUM1 Promotes Tumorigenesis and Progression of Ovarian Cancer by Sponging miR-615-5p and miR-6753-5p.

Molecular therapy. Nucleic acids

Guan X,Zong ZH,Liu Y,Chen S,Wang LL,Zhao Y

Fri Dec 06 00:00:00 EST 2019

Semaphorin 4C Promotes Macrophage Recruitment and Angiogenesis in Breast Cancer.

Molecular cancer research : MCR

Yang J,Zeng Z,Qiao L,Jiang X,Ma J,Wang J,Ye S,Ma Q,Wei J,Wu M,Huang X,Ma D,Gao Q

Tue Oct 01 00:00:00 EDT 2019

SHMT2 and the BRCC36/BRISC deubiquitinase regulate HIV-1 Tat K63-ubiquitylation and destruction by autophagy.

PLoS pathogens

Xu M,Moresco JJ,Chang M,Mukim A,Smith D,Diedrich JK,Yates JR,Jones KA

Tue May 01 00:00:00 EDT 2018

SHMT2 and the BRCC36/BRISC deubiquitinase regulate HIV-1 Tat K63-ubiquitylation and destruction by autophagy.

PLoS pathogens

Xu M,Moresco JJ,Chang M,Mukim A,Smith D,Diedrich JK,Yates JR,Jones KA

Tue May 01 00:00:00 EDT 2018

A regulatory mutant on TRIM26 conferring the risk of nasopharyngeal carcinoma by inducing low immune response.

Cancer medicine

Lyu XM,Zhu XW,Zhao M,Zuo XB,Huang ZX,Liu X,Jiang T,Yang XX,Li X,Long XB,Wang JG,Li JB,Han MY,Wang S,Liu TF,Zhang B,Sun T,Cheng Z,Qiu MC,Dong L,Zheng L,Zhang LC,Wang JH,Wei GG,Yao K,Wang Q,Zheng HF,Li X

Wed Aug 01 00:00:00 EDT 2018

A regulatory mutant on TRIM26 conferring the risk of nasopharyngeal carcinoma by inducing low immune response.

Cancer medicine

Wed Aug 01 00:00:00 EDT 2018

A proteomics landscape of circadian clock in mouse liver.

Nature communications

Wang Y,Song L,Liu M,Ge R,Zhou Q,Liu W,Li R,Qie J,Zhen B,Wang Y,He F,Qin J,Ding C

Thu Apr 19 00:00:00 EDT 2018

A proteomics landscape of circadian clock in mouse liver.

Nature communications

Wang Y,Song L,Liu M,Ge R,Zhou Q,Liu W,Li R,Qie J,Zhen B,Wang Y,He F,Qin J,Ding C

Thu Apr 19 00:00:00 EDT 2018

Lymphotoxins Promote the Progression of Human Lymphatic Malformation by Enhancing Lymphatic Endothelial Cell Proliferation.

The American journal of pathology

Yang JG,Sun YF,He KF,Ren JG,Liu ZJ,Liu B,Zhang W,Zhao YF

Wed Nov 01 00:00:00 EDT 2017

Lymphotoxins Promote the Progression of Human Lymphatic Malformation by Enhancing Lymphatic Endothelial Cell Proliferation.

The American journal of pathology

Yang JG,Sun YF,He KF,Ren JG,Liu ZJ,Liu B,Zhang W,Zhao YF

Wed Nov 01 00:00:00 EDT 2017

Lymphotoxins Promote the Progression of Human Lymphatic Malformation by Enhancing Lymphatic Endothelial Cell Proliferation.

The American journal of pathology

Yang JG,Sun YF,He KF,Ren JG,Liu ZJ,Liu B,Zhang W,Zhao YF

Wed Nov 01 00:00:00 EDT 2017

Identification of 11(13)-dehydroivaxillin as a potent therapeutic agent against non-Hodgkin's lymphoma.

Cell death & disease

Xiao X,Li H,Jin H,Jin J,Yu M,Ma C,Tong Y,Zhou L,Lei H,Xu H,Zhang W,Liu W,Wu Y

Thu Sep 14 00:00:00 EDT 2017

Identification of 11(13)-dehydroivaxillin as a potent therapeutic agent against non-Hodgkin's lymphoma.

Cell death & disease

Xiao X,Li H,Jin H,Jin J,Yu M,Ma C,Tong Y,Zhou L,Lei H,Xu H,Zhang W,Liu W,Wu Y

Thu Sep 14 00:00:00 EDT 2017

Dioscin attenuates hepatic ischemia-reperfusion injury in rats through inhibition of oxidative-nitrative stress, inflammation and apoptosis.

Transplantation

Tao X,Wan X,Xu Y,Xu L,Qi Y,Yin L,Han X,Lin Y,Peng J

Sat Sep 27 00:00:00 EDT 2014

Dioscin attenuates hepatic ischemia-reperfusion injury in rats through inhibition of oxidative-nitrative stress, inflammation and apoptosis.

Transplantation

Tao X,Wan X,Xu Y,Xu L,Qi Y,Yin L,Han X,Lin Y,Peng J

Sat Sep 27 00:00:00 EDT 2014

Neuroprotective effects of rhynchophylline against ischemic brain injury via regulation of the Akt/mTOR and TLRs signaling pathways.

Molecules (Basel, Switzerland)

Huang H,Zhong R,Xia Z,Song J,Feng L

Wed Jul 30 00:00:00 EDT 2014

Neuroprotective effects of rhynchophylline against ischemic brain injury via regulation of the Akt/mTOR and TLRs signaling pathways.

Molecules (Basel, Switzerland)

Huang H,Zhong R,Xia Z,Song J,Feng L

Wed Jul 30 00:00:00 EDT 2014

Neuroprotective effects of rhynchophylline against ischemic brain injury via regulation of the Akt/mTOR and TLRs signaling pathways.

Molecules (Basel, Switzerland)

Huang H,Zhong R,Xia Z,Song J,Feng L

Wed Jul 30 00:00:00 EDT 2014

Neuroprotective effects of rhynchophylline against ischemic brain injury via regulation of the Akt/mTOR and TLRs signaling pathways.

Molecules (Basel, Switzerland)

Huang H,Zhong R,Xia Z,Song J,Feng L

Wed Jul 30 00:00:00 EDT 2014

Bioinformatics

Protein Aliases: DNA-binding factor KBF2; H2TF1; Lymphocyte translocation chromosome 10 protein; Lyt10; NF kappaB2; NF KB; NFKB, p52/p100 subunit; nuclear factor; nuclear factor kappa b; nuclear factor Kappa-B, subunit 2; Nuclear factor NF-kappa-B p100 subunit; nuclear factor NF-kappa-B p52 subunit; nuclear factor of Kappa light chain gene enhancer in B cells 2; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 2; nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49/p100); nuclear factor of kappa light polypeptide gene enhancer in B-cells 2, p49/p100; Oncogene Lyt-10; OTTHUMP00000020371; transcription factor NFKB2

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Gene Aliases: CVID10; H2TF1; lyt; LYT-10; LYT10; NF-kappaB2; NF-kB2; NFKB2; p100; p49; p49/p100; p50B; p52; RGD1307189

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UniProt ID: (Human) Q00653, (Mouse) Q9WTK5

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Entrez Gene ID: (Human) 4791, (Rat) 309452, (Mouse) 18034

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Function(s)

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding chromatin binding transcription factor activity, sequence-specific DNA binding transcription coactivator activity protein binding RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding DNA binding Rel homology transcription factor

Process(es)

negative regulation of transcription from RNA polymerase II promoter follicular dendritic cell differentiation germinal center formation regulation of transcription, DNA-templated transcription from RNA polymerase II promoter inflammatory response I-kappaB kinase/NF-kappaB signaling aging extracellular matrix organization positive regulation of type I interferon production response to lipopolysaccharide response to cytokine NIK/NF-kappaB signaling innate immune response positive regulation of transcription from RNA polymerase II promoter rhythmic process spleen development positive regulation of NF-kappaB transcription factor activity transcription, DNA-templated regulation of transcription from RNA polymerase II promoter signal transduction lymph node development

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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NFKB2 Polyclonal Antibody

NFKB2 Antibody (10409-2-AP) in ICC/IF

Product Details

10409-2-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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