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Product Details
500-3634
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corrsponding to the internal segment of humanNFkBp50
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with proprietary stabilizer
Contains
0.01% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
Product Specific Information
Positive control: all human tissue
Cellular location: Nucleus, Cytoplasm.
Target Information
NFkB is a nuclear transcription factor activated by various extra and intracellular stimuli such as cytokines, UV radiation, stress, in injury, and by bacterial and viral products. NFkB is involved in regulation of various cellular events including cell growth, differentiation, proliferation, apoptosis and inflammation. NFKB1 (p50) is a 50KDa functional sub-unit of NF-kB and a member of Rel protein family which is synthesized as a p105 precursor protein and consists of an N-terminal conserved RHD-region containing nuclear localization signal, DNA-binding and dimerization domains. NFKB1 (p50) forms homodimers or heterodimerizes with p65, forming the functional NFkB factor. Further, NFKB1 (p50) directs the nuclear translocation of NF-kB and is instrumental in its DNA-binding. The NFkB complex is expressed in most cell types, and is primarily found in the cytoplasm in an inactive state in association with I-kappa-B. Phosphorylation of I-kappa-B releases the complex, allowing translocation to the nucleus and modification of gene expression. Phosphorylation of the p50 subunit at the Ser-337 residue has been shown to significantly increase DNA binding efficiency of the NFkB protein complex. The pathological role of NFkB has been suggested in AIDS, hematogenic cancer cell metastasis and rheumatoid arthritis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DKFZp686C01211; DNA-binding factor KBF1; EBP-1; MGC54151; NF KB; NF-kappabeta; NF-kB p50; nuclear factor; nuclear factor kappa b; nuclear factor kappa-B DNA binding subunit; Nuclear factor NF-kappa-B p105 subunit; nuclear factor NF-kappa-B p50 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1; OTTHUMP00000219572
Gene Aliases: CVID12; EBP-1; KBF1; NF-kappa-B; NF-kappaB; NF-kB1; NFkappaB; NFKB-p105; NFKB-p50; NFKB1; p105; p50
UniProt ID: (Human) P19838
Entrez Gene ID: (Human) 4790
regulatory region DNA binding
transcription regulatory region sequence-specific DNA binding
RNA polymerase II regulatory region sequence-specific DNA binding
RNA polymerase II distal enhancer sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II distal enhancer sequence-specific binding
transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding
chromatin binding
transcription factor activity, sequence-specific DNA binding
protein binding
transcription factor binding
heat shock protein binding
identical protein binding
protein homodimerization activity
actinin binding
transcription regulatory region DNA binding
protein heterodimerization activity
Rel homology transcription factor
negative regulation of transcription from RNA polymerase II promoter
stimulatory C-type lectin receptor signaling pathway
transcription from RNA polymerase II promoter
apoptotic process
inflammatory response
response to oxidative stress
I-kappaB kinase/NF-kappaB signaling
positive regulation of macrophage derived foam cell differentiation
positive regulation of lipid storage
negative regulation of calcidiol 1-monooxygenase activity
negative regulation of vitamin D biosynthetic process
membrane protein intracellular domain proteolysis
negative regulation of cellular protein metabolic process
negative regulation of cholesterol transport
positive regulation of type I interferon production
response to muscle stretch
NIK/NF-kappaB signaling
Fc-epsilon receptor signaling pathway
negative regulation of apoptotic process
negative regulation of interleukin-12 biosynthetic process
innate immune response
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
response to copper ion
negative regulation of inflammatory response
T cell receptor signaling pathway
positive regulation of NF-kappaB transcription factor activity
stress-activated MAPK cascade
cellular response to lipopolysaccharide
cellular response to mechanical stimulus
cellular response to nicotine
cellular response to carbohydrate stimulus
cellular response to interleukin-1
cellular response to interleukin-6
cellular response to tumor necrosis factor
cellular response to dsRNA
cellular response to peptide hormone stimulus
positive regulation of canonical Wnt signaling pathway
positive regulation of hyaluronan biosynthetic process
cellular response to diterpene
cellular response to glucoside
cellular response to brain-derived neurotrophic factor stimulus
positive regulation of miRNA metabolic process
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