Invitrogen

NFkB p50 Polyclonal Antibody

Advanced Verification

IP-mass spectrometry

10 Published Figures

13 References

View all (15) NFkB p50 antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

NFkB p50 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 15

NFkB p50 Antibody (51-3500) in ICC/IF

Immunofluorescent analysis of NFkB p105/p50 Antibody was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with NFkB p105/p50 Antibody (Product # 38-5600) at 1µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Fluor 488 Goat Anti-Rabbit IgG Secondary Antibody (Product # A-11008) at a dilution of 1:400 for 45 minute... View More

NFkB p50 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

NFkB p50 Antibody in Immunocytochemistry, Immunohistochemistry, Flow Cytometry (ICC/IF, IHC, Flow)

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Product Details

51-3500

Applications

Tested Dilution

Publications

Western Blot (WB)

1.0 µg/mL

View 10 publications 10 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1 µg/mL

View 1 publication 1 publication

Flow Cytometry (Flow)

View 1 publication 1 publication

ELISA (ELISA)

0.1-1.0 µg/mL

Gel Shift (GS)

5-10 µg/mL

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse, Rat, Virus

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein derived from an internal region of the human NF-kappa-B (p50) (nuclear factor of kappa-B).

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533906

Target Information

NFkB is a nuclear transcription factor activated by various extra and intracellular stimuli such as cytokines, UV radiation, stress, in injury, and by bacterial and viral products. NFkB is involved in regulation of various cellular events including cell growth, differentiation, proliferation, apoptosis and inflammation. NFKB1 (p50) is a 50KDa functional sub-unit of NF-kB and a member of Rel protein family which is synthesized as a p105 precursor protein and consists of an N-terminal conserved RHD-region containing nuclear localization signal, DNA-binding and dimerization domains. NFKB1 (p50) forms homodimers or heterodimerizes with p65, forming the functional NFkB factor. Further, NFKB1 (p50) directs the nuclear translocation of NF-kB and is instrumental in its DNA-binding. The NFkB complex is expressed in most cell types, and is primarily found in the cytoplasm in an inactive state in association with I-kappa-B. Phosphorylation of I-kappa-B releases the complex, allowing translocation to the nucleus and modification of gene expression. Phosphorylation of the p50 subunit at the Ser-337 residue has been shown to significantly increase DNA binding efficiency of the NFkB protein complex. The pathological role of NFkB has been suggested in AIDS, hematogenic cancer cell metastasis and rheumatoid arthritis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Nobiletin and xanthohumol counteract the TNFα-mediated activation of endothelial cells through the inhibition of the NF-κB signaling pathway.

Cell biology international

Corrado C,Barreca MM,Raimondo S,Diana P,Pepe G,Basilicata MG,Conigliaro A,Alessandro R

Published figure using NFkB p50 polyclonal antibody (Product # 51-3500) in Western Blot

Wed Mar 01 00:00:00 EST 2023

The critical role of KLF4 in regulating the activation of A1/A2 reactive astrocytes following ischemic stroke.

Journal of neuroinflammation

Wang C,Li L

51-3500 was used in Western Blotting to demonstrate that astrocytic KLF4 expression and complement C3-positive A1 and S100 calcium binding protein A10 (S100A10)-positive A2 astrocytes were induced in the ischemic penumbra following focal cerebral ischemia, and the time course of upregulation of astrocytic KLF4 correlated closely with the activation of A2 astrocytes.

Thu Feb 23 00:00:00 EST 2023

The critical role of KLF4 in regulating the activation of A1/A2 reactive astrocytes following ischemic stroke.

Journal of neuroinflammation

Wang C,Li L

Thu Feb 23 00:00:00 EST 2023

Silencing of lncRNA XIST impairs angiogenesis and exacerbates cerebral vascular injury after ischemic stroke.

Molecular therapy. Nucleic acids

Wang C,Dong J,Sun J,Huang S,Wu F,Zhang X,Pang D,Fu Y,Li L

Published figure using NFkB p50 polyclonal antibody (Product # 51-3500) in Western Blot

Fri Dec 03 00:00:00 EST 2021

Silencing of lncRNA XIST impairs angiogenesis and exacerbates cerebral vascular injury after ischemic stroke.

Molecular therapy. Nucleic acids

Wang C,Dong J,Sun J,Huang S,Wu F,Zhang X,Pang D,Fu Y,Li L

Published figure using NFkB p50 polyclonal antibody (Product # 51-3500) in Western Blot

Fri Dec 03 00:00:00 EST 2021

KLF4 alleviates cerebral vascular injury by ameliorating vascular endothelial inflammation and regulating tight junction protein expression following ischemic stroke.

Journal of neuroinflammation

Zhang X,Wang L,Han Z,Dong J,Pang D,Fu Y,Li L

51-3500 was used in Western Blotting to examine the correlation between serum levels of CAMs/KLF4 and infarct volume in acute CIS patients.

Tue Apr 07 00:00:00 EDT 2020

KLF4 alleviates cerebral vascular injury by ameliorating vascular endothelial inflammation and regulating tight junction protein expression following ischemic stroke.

Journal of neuroinflammation

Zhang X,Wang L,Han Z,Dong J,Pang D,Fu Y,Li L

51-3500 was used in Western Blotting to examine the correlation between serum levels of CAMs/KLF4 and infarct volume in acute CIS patients.

Tue Apr 07 00:00:00 EDT 2020

Establishment of three novel cell lines derived from African American patients with colorectal carcinoma: A unique tool for assessing racial health disparity.

International journal of oncology

Paredes J,Ji P,Lacomb JF,Shroyer KR,Martello LA,Williams JL

Published figure using NFkB p50 polyclonal antibody (Product # 51-3500) in Western Blot

Mon Oct 01 00:00:00 EDT 2018

Photobiomodulation with Pulsed and Continuous Wave Near-Infrared Laser (810 nm, Al-Ga-As) Augments Dermal Wound Healing in Immunosuppressed Rats.

PloS one

Keshri GK,Gupta A,Yadav A,Sharma SK,Singh SB

513500 was used in western blot to report the photobiomodulatory effects of 810 nm diode laser and continuous wave on full-thickness excision-type dermal wound healing in hydrocortisone-induced immunosuppressed rats

Mon Jun 26 00:00:00 EDT 2017

Superpulsed (Ga-As, 904 nm) low-level laser therapy (LLLT) attenuates inflammatory response and enhances healing of burn wounds.

Journal of biophotonics

Gupta A,Keshri GK,Yadav A,Gola S,Chauhan S,Salhan AK,Bala Singh S

51-3500 was used in western blot to examine the effects of superpulsed laser on the healing of burn wounds in rats and investigate the associated mechanisms of action.

Mon Jun 01 00:00:00 EDT 2015

Inhibition of rotavirus ECwt infection in ICR suckling mice by N-acetylcysteine, peroxisome proliferator-activated receptor gamma agonists and cyclooxygenase-2 inhibitors.

Memorias do Instituto Oswaldo Cruz

Guerrero CA,Paula Pardo VR,Rafael Guerrero OA

Published figure using NFkB p50 polyclonal antibody (Product # 51-3500) in Flow Cytometry

Sun Sep 01 00:00:00 EDT 2013

Inhibition of rotavirus ECwt infection in ICR suckling mice by N-acetylcysteine, peroxisome proliferator-activated receptor gamma agonists and cyclooxygenase-2 inhibitors.

Memorias do Instituto Oswaldo Cruz

Guerrero CA,Paula Pardo VR,Rafael Guerrero OA

Published figure using NFkB p50 polyclonal antibody (Product # 51-3500) in Flow Cytometry

Sun Sep 01 00:00:00 EDT 2013

Inhibition of rotavirus ECwt infection in ICR suckling mice by N-acetylcysteine, peroxisome proliferator-activated receptor gamma agonists and cyclooxygenase-2 inhibitors.

Memorias do Instituto Oswaldo Cruz

Guerrero CA,Paula Pardo VR,Rafael Guerrero OA

Published figure using NFkB p50 polyclonal antibody (Product # 51-3500) in Flow Cytometry

Sun Sep 01 00:00:00 EDT 2013

Human gut flora-fermented nondigestible fraction from cooked bean ( Phaseolus vulgaris L.) modifies protein expression associated with apoptosis, cell cycle arrest, and proliferation in human adenocarcinoma colon cancer cells.

Journal of agricultural and food chemistry

Campos-Vega R,García-Gasca T,Guevara-Gonzalez R,Ramos-Gomez M,Oomah BD,Loarca-Piña G

51-3500 was used in western blot to assess the effects of fermentation products produced by the human gut flora on human adenocarcinoma colon cancer cells.

Wed Dec 26 00:00:00 EST 2012

Measles virus-induced modulation of host-cell gene expression.

The Journal of general virology

Bolt G,Berg K,Blixenkrone-Møller M

Wed May 01 00:00:00 EDT 2002

Measles virus-induced modulation of host-cell gene expression.

The Journal of general virology

Bolt G,Berg K,Blixenkrone-Møller M

Wed May 01 00:00:00 EDT 2002

Mediation of proliferating cell nuclear antigen (PCNA)-dependent DNA replication through a conserved p21(Cip1)-like PCNA-binding motif present in the third subunit of human DNA polymerase delta.

The Journal of biological chemistry

Ducoux M,Urbach S,Baldacci G,Hübscher U,Koundrioukoff S,Christensen J,Hughes P

Fri Dec 28 00:00:00 EST 2001

Mediation of proliferating cell nuclear antigen (PCNA)-dependent DNA replication through a conserved p21(Cip1)-like PCNA-binding motif present in the third subunit of human DNA polymerase delta.

The Journal of biological chemistry

Ducoux M,Urbach S,Baldacci G,Hübscher U,Koundrioukoff S,Christensen J,Hughes P

Fri Dec 28 00:00:00 EST 2001

Bioinformatics

Protein Aliases: DKFZp686C01211; DNA-binding factor KBF1; EBP-1; MGC54151; NF kappaB1; NF KB; NF-kappa-B1 p84/NF-kappa-B1 p98; NF-kappaB p50; NF-kappabeta; nuclear factor; nuclear factor kappa b; nuclear factor kappa-B DNA binding subunit; nuclear factor kappaB p50; Nuclear factor NF-kappa-B p105 subunit; nuclear factor NF-kappa-B p50 subunit; nuclear factor of kappa light chain gene enhancer in B-cells 1, p105; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1; nuclear factor of kappa light polypeptide gene enhancer in B-cells 1, p105; nuclear factor-kappaB p50; OTTHUMP00000219572; p50 subunit of NF kappaB; p50 subunit of NF-kappaB

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Gene Aliases: CVID12; EBP-1; KBF1; NF-kappa-B; NF-kappaB; NF-kappaB1; NF-KB1; NFkappaB; NFKB-p105; NFKB-p50; NFKB1; p105; p50; p50/p105

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UniProt ID: (Human) P19838, (Mouse) P25799

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Entrez Gene ID: (Human) 4790, (Mouse) 18033

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Function(s)

regulatory region DNA binding transcription regulatory region sequence-specific DNA binding RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II distal enhancer sequence-specific DNA binding transcriptional activator activity, RNA polymerase II distal enhancer sequence-specific binding transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding chromatin binding transcription factor activity, sequence-specific DNA binding protein binding transcription factor binding heat shock protein binding identical protein binding protein homodimerization activity actinin binding transcription regulatory region DNA binding protein heterodimerization activity RNA polymerase II transcription factor activity, sequence-specific DNA binding DNA binding double-stranded DNA binding sequence-specific DNA binding Rel homology transcription factor

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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NFkB p50 Polyclonal Antibody

NFkB p50 Antibody (51-3500) in ICC/IF

Product Details

51-3500

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

Gel Shift (GS)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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