Invitrogen

NFkB p50/p105 Recombinant Rabbit Monoclonal Antibody (RM299)

View all (45) NFkB p50/p105 antibodies
Datasheet
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Datasheet
Protocols
Questions & Answers

Product Details

MA5-33067

Applications
Tested Dilution
Publications

Western Blot (WB)

1:100-1:200
-

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:200
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

RM299

Immunogen

A peptide corresponding to residues in the human NFkappa-B p105 subunit and p50 subunit.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.1 mg/mL

Purification

Protein A

Storage buffer

PBS with 1% BSA, 50% glycerol

Contains

0.09% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2810160

Product Specific Information

Specificity: reacts to human nuclear factor NF-kappaB p105 subunit and p50 subunit. It may also react to mouse or rat NF-kappa-B p105/p50, as predicted by immunogen homology.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

NF-kB is a nuclear transcription factor activated by various extra and intracellular stimuli such as cytokines, UV radiation, stress, in injury, and by bacterial and viral products. It is involved in regulation of various cellular events including cell growth, differentiation, proliferation, apoptosis and inflammation. NFKB1 (p50), a 50KDa functional sub-unit of NF-kB, is a member of Rel protein family. It is synthesized as a p105 precursor protein and consists of an N-terminal conserved RHD-region containing nuclear localization signal, DNA-binding and dimerization domains. NFKB1 (p50) forms homodimers or heterodimerizes with p65, forming the functional NF-kB factor. NFKB1 (p50) directs the nuclear translocation of NF-kB and is instrumental in its DNA-bidning. Pathological role of NF-kB has been suggested in AIDS, hematogenic cancer cell metastasis and rheumatoid arthritis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: DKFZp686C01211; DNA-binding factor KBF1; EBP-1; MGC54151; NF KB; NF-kappabeta; NF-kB p50/p105; nuclear factor; nuclear factor kappa b; nuclear factor kappa-B DNA binding subunit; Nuclear factor NF-kappa-B p105 subunit; nuclear factor NF-kappa-B p50 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1

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Gene Aliases: CVID12; EBP-1; KBF1; NF-kappa-B; NF-kappaB; NF-kB1; NFkappaB; NFKB-p105; NFKB-p50; NFKB1; p105; p50

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UniProt ID: (Human) P19838

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Entrez Gene ID: (Human) 4790

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Function(s)
regulatory region DNA binding transcription regulatory region sequence-specific DNA binding RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II distal enhancer sequence-specific DNA binding transcriptional activator activity, RNA polymerase II distal enhancer sequence-specific binding transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding chromatin binding transcription factor activity, sequence-specific DNA binding protein binding transcription factor binding heat shock protein binding identical protein binding protein homodimerization activity actinin binding transcription regulatory region DNA binding protein heterodimerization activity Rel homology transcription factor
Process(es)
negative regulation of transcription from RNA polymerase II promoter stimulatory C-type lectin receptor signaling pathway transcription from RNA polymerase II promoter apoptotic process inflammatory response response to oxidative stress I-kappaB kinase/NF-kappaB signaling positive regulation of macrophage derived foam cell differentiation positive regulation of lipid storage negative regulation of calcidiol 1-monooxygenase activity negative regulation of vitamin D biosynthetic process membrane protein intracellular domain proteolysis negative regulation of cellular protein metabolic process negative regulation of cholesterol transport positive regulation of type I interferon production response to muscle stretch NIK/NF-kappaB signaling Fc-epsilon receptor signaling pathway negative regulation of apoptotic process negative regulation of interleukin-12 biosynthetic process innate immune response positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter response to copper ion negative regulation of inflammatory response T cell receptor signaling pathway positive regulation of NF-kappaB transcription factor activity stress-activated MAPK cascade cellular response to lipopolysaccharide cellular response to mechanical stimulus cellular response to nicotine cellular response to carbohydrate stimulus cellular response to interleukin-1 cellular response to interleukin-6 cellular response to tumor necrosis factor cellular response to dsRNA cellular response to peptide hormone stimulus positive regulation of canonical Wnt signaling pathway positive regulation of hyaluronan biosynthetic process cellular response to diterpene cellular response to glucoside cellular response to brain-derived neurotrophic factor stimulus positive regulation of miRNA metabolic process
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