Invitrogen

NFkB p65 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

13 Published Figures

50 References

View all (154) NFkB p65 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

NFkB p65 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 20

NFkB p65 Antibody (PA5-16545) in ICC/IF

Immunofluorescent analysis of NFkappaB/p65 (green) in HeLa cells either left untreated (left panel) or treated with 25 ng/mL of TNF-alpha (right panel) for 25 minutes. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with a NFkappaB/p65 polyclonal antibody (Product # PA5-16545) at a dilution of 1:50 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight... View More

NFkB p65 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

NFkB p65 Antibody in Western Blot, Immunocytochemistry, Immunohistochemistry (WB, ICC/IF, IHC)

NFkB p65 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

NFkB p65 Antibody in Immunohistochemistry (IHC)

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Product Details

PA5-16545

Applications

Tested Dilution

Publications

Western Blot (WB)

5-10 µg/mL

View 17 publications 17 publications

Immunohistochemistry (IHC)

View 19 publications 19 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:100

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

1:50-1:200

View 11 publications 11 publications

CUT&RUN (C&R)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Bovine, Dog, Fruit fly, Hamster, Human, Mouse, Pig, Rabbit, Rat, Xenopus

Published species

Bovine, Human, Mouse, Pig, Rabbit, Rat, Zebrafish

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide from the C-terminus of human NFkappaB protein

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice (domestic); Dry ice (international)

RRID

AB_10981081

Product Specific Information

PA5-16545 targets NFkappaB/p65 in IHC (P), IF, and WB applications and shows reactivity with mouse, Rabbit, Rat, Xn, Bovine, Canine, Drosophila, Hamster, Human, and Porcine samples.

The PA5-16545 immunogen is a synthetic peptide from the C-terminus of human NFkappaB protein.

This antibody was orginally validated as part of a Thermo Scientific Cellomics High Content Screening Kit. The antibody sold separately may have slightly different performance and may need to be further optimized for the best results.

Target Information

Nuclear factor kB p65 (NF-kB p65) is encoded by the RELA gene and is present on chromosome 11 in humans. NF-kB P65 is also known as RelA (v-rel avian reticuloendotheliosis viral oncogene homolog A) and belongs to the Rel family of proteins. It is one of the two subunits of NF-kB (Nuclear factor kappa-light-chain-enhancer of activated B cells) that heterodimerizes with the other subunits p50 or p52. However, binding of TNF alpha to its cognate receptor phosphorylates IKK which in turn phosphorylates I kB allowing proteasomal degradation of I kB. It specifically plays a key role in transcription of immunoglobulin k (kappa) gene in mature B-lymphoid cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

LOX-1 mediates inflammatory activation of microglial cells through the p38-MAPK/NF-κB pathways under hypoxic-ischemic conditions.

Cell communication and signaling : CCS

Aoki Y,Dai H,Furuta F,Akamatsu T,Oshima T,Takahashi N,Goto YI,Oka A,Itoh M

Published figure using NFkB p65 polyclonal antibody (Product # PA5-16545) in Western Blot

Fri Jun 02 00:00:00 EDT 2023

Targeting clock-controlled gene Nrf2 ameliorates inflammation-induced intervertebral disc degeneration.

Arthritis research & therapy

Peng P,Wang D,Xu X,Wang D,Gao B,Wang H,Jia H,Shang Q,Zheng C,Gao C,Mao J,Luo Z,Yang L,Hu X

PA5-16545 was used in Western Blot, Immunohistochemistry to induce intervertebral disc degeneration and found that the Intervertebral disc clock was dampened in degenerated human nucleus pulposus specimens, rat nucleus pulposus tissues, and cells.

Wed Aug 03 00:00:00 EDT 2022

Targeting clock-controlled gene Nrf2 ameliorates inflammation-induced intervertebral disc degeneration.

Arthritis research & therapy

Peng P,Wang D,Xu X,Wang D,Gao B,Wang H,Jia H,Shang Q,Zheng C,Gao C,Mao J,Luo Z,Yang L,Hu X

Wed Aug 03 00:00:00 EDT 2022

Study on Toll-Like Receptor 2-Mediated Inflammation-Induced Familial Hypertension Combined with Hyperlipemia and Its Mechanism.

Journal of healthcare engineering

Liu J,Li C,Wang Q,Hu H,Li C,Qian J

PA5-16545 was used in Western Blot to investigate the influence of Toll-like receptor 2- (TLR2-) mediated inflammation on the occurrence and development of familial hypertension combined with hyperlipemia and its related mechanism.

Thu Apr 21 00:00:00 EDT 2022

Beneficial effect of trimetazidine on folic acid-induced acute kidney injury in mice: Role of HIF-1α/HO-1.

Journal of biochemical and molecular toxicology

Abdelrahman RS,Abdelsalam RA,Zaghloul MS

PA5-16545 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to investigate the impact of trimetazidine (a metabolic modulator-antianginal drug), against folic acid-induced acute kidney injury.

Sun May 01 00:00:00 EDT 2022

Kukoamine A inhibits C-C motif chemokine receptor 5 to attenuate lipopolysaccharide-induced lung injury.

Drug development research

Liu X,Wang M,Song Y,Zhang C,Jiang Y,Li W,Xu B,Jiang Z

Published figure using NFkB p65 polyclonal antibody (Product # PA5-16545) in Western Blot

Thu Sep 01 00:00:00 EDT 2022

Kukoamine A inhibits C-C motif chemokine receptor 5 to attenuate lipopolysaccharide-induced lung injury.

Drug development research

Liu X,Wang M,Song Y,Zhang C,Jiang Y,Li W,Xu B,Jiang Z

Published figure using NFkB p65 polyclonal antibody (Product # PA5-16545) in Western Blot

Thu Sep 01 00:00:00 EDT 2022

Identification of Transcription Factor Binding Sites by Cleavage Under Target and Release Using Nuclease in Zebrafish.

Zebrafish

Barakat R,Campbell CA,Espin-Palazon R

PA5-16545 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot, ChIP Assay to provide a detailed workflow for the identification of predicted TF binding sites that can be utilized in any animal species and show a strategy which will expand this invaluable tool to the zebrafish community, improving the epigenetic resolution that can be achieved in this model organism.

Wed Jun 01 00:00:00 EDT 2022

Nucleic acid aptamer controls mycoplasma infection for inhibiting the malignancy of esophageal squamous cell carcinoma.

Molecular therapy : the journal of the American Society of Gene Therapy

Zhang Y,Zhang H,Sun X,Peng T,Xie T,Yuan Y,Guo J,Chen Y,Zhou L,Ling N,Li H,Li L,Zhang L,Li X,Liang L,Liu J,Ye M,Tan W

PA5-16545 was used in Western Blotting to indicate that aptamer ZY3A is a potential candidate for development into a novel molecular tool for treatment of M. hyorhinis infection and a safe first-in-class M. hyorhinis-targeting antitumor agent.

Wed Jun 01 00:00:00 EDT 2022

Dapagliflozin, Liraglutide, and Their Combination Attenuate Diabetes Mellitus-Associated Hepato-Renal Injury-Insight into Oxidative Injury/Inflammation/Apoptosis Modulation.

Life (Basel, Switzerland)

El-Sherbiny M,El-Shafey M,Said E,Shaker GA,El-Dosoky M,Ebrahim HA,Abed SY,Ibraheem KM,Mohsen Faheem A,AlMutawa M,Alatawi B,Elsherbiny NM

PA5-16545 was used in Immunohistochemistry to explore the beneficial therapeutic impacts of dapagliflozin (Dapa), a highly potent, reversible, and selective sodium-glucose cotransporter-2 inhibitor, and liraglutide (Lira), a glucagon-like peptide-1 (GLP-1) receptor agonist, as hypoglycaemic agents for the management of diabetes mellitus (DM), as well as their combination against DM-induced complications, including hepato-renal injury.

Sat May 21 00:00:00 EDT 2022

Stingray Venom Proteins: Mechanisms of Action Revealed Using a Novel Network Pharmacology Approach.

Marine drugs

Kirchhoff KN,Billion A,Voolstra CR,Kremb S,Wilke T,Vilcinskas A

Published figure using NFkB p65 polyclonal antibody (Product # PA5-16545) in Immunocytochemistry

Fri Dec 24 00:00:00 EST 2021

Stingray Venom Proteins: Mechanisms of Action Revealed Using a Novel Network Pharmacology Approach.

Marine drugs

Kirchhoff KN,Billion A,Voolstra CR,Kremb S,Wilke T,Vilcinskas A

Published figure using NFkB p65 polyclonal antibody (Product # PA5-16545) in Immunocytochemistry

Fri Dec 24 00:00:00 EST 2021

Bacterial lipopolysaccharides can initiate regeneration of the Xenopus tadpole tail.

iScience

Bishop TF,Beck CW

Published figure using NFkB p65 polyclonal antibody (Product # PA5-16545) in Western Blot

Fri Nov 19 00:00:00 EST 2021

Capsaicin Attenuates Lipopolysaccharide-Induced Inflammation and Barrier Dysfunction in Intestinal Porcine Epithelial Cell Line-J2.

Frontiers in physiology

Zhao X,Dong B,Friesen M,Liu S,Zhu C,Yang C

Published figure using NFkB p65 polyclonal antibody (Product # PA5-16545) in Western Blot

Tue Oct 12 00:00:00 EDT 2021

Protective Effects of Bariatric Surgery on Kidney Functions by Inhibiting Oxidative Stress Responses Through Activating PPARα in Rats With Diabetes.

Frontiers in physiology

Jiang HW,Zhou Y,Zhou PY,Zhang TY,Hu JY,Bai XT

Published figure using NFkB p65 polyclonal antibody (Product # PA5-16545) in Immunohistochemistry

Sun Apr 24 00:00:00 EDT 2022

Protective Effects of Bariatric Surgery on Kidney Functions by Inhibiting Oxidative Stress Responses Through Activating PPARα in Rats With Diabetes.

Frontiers in physiology

Jiang HW,Zhou Y,Zhou PY,Zhang TY,Hu JY,Bai XT

Published figure using NFkB p65 polyclonal antibody (Product # PA5-16545) in Immunohistochemistry

Sun Apr 24 00:00:00 EDT 2022

Intrarenal modulation of NF-κB activity attenuates cardiac injury in a swine model of CKD: a renal-cardio axis.

American journal of physiology. Renal physiology

Chade AR,Engel JE,Hall ME,Eirin A,Bidwell GL

PA5-16545 was used in Immunohistochemistry to support the role of inflammatory cytokines of renal origin in renal-cardio pathophysiology in CKD and that the heart may be a target.

Fri Oct 01 00:00:00 EDT 2021

A-kinase-interacting protein 1 promotes EMT and metastasis via PI3K/Akt/IKKβ pathway in cervical cancer.

Cell biochemistry and function

Zhang X,Liu S,Zhu Y

Published figure using NFkB p65 polyclonal antibody (Product # PA5-16545) in Western Blot

Sat Aug 01 00:00:00 EDT 2020

Saururus chinensis-controlled allergic pulmonary disease through NF-κB/COX-2 and PGE₂ pathways.

PeerJ

Song M,Lee SY,Kim M,Park S,Park J,Kwon Y,Park DH

Published figure using NFkB p65 polyclonal antibody (Product # PA5-16545) in Immunocytochemistry

Fri Oct 09 00:00:00 EDT 2020

Saururus chinensis-controlled allergic pulmonary disease through NF-κB/COX-2 and PGE₂ pathways.

PeerJ

Song M,Lee SY,Kim M,Park S,Park J,Kwon Y,Park DH

Published figure using NFkB p65 polyclonal antibody (Product # PA5-16545) in Immunocytochemistry

Fri Oct 09 00:00:00 EDT 2020

Pulsed focused ultrasound enhances the therapeutic effect of mesenchymal stromal cell-derived extracellular vesicles in acute kidney injury.

Stem cell research & therapy

Ullah M,Liu DD,Rai S,Razavi M,Concepcion W,Thakor AS

Published figure using NFkB p65 polyclonal antibody (Product # PA5-16545) in Western Blot

Mon Sep 14 00:00:00 EDT 2020

Pulsed focused ultrasound enhances the therapeutic effect of mesenchymal stromal cell-derived extracellular vesicles in acute kidney injury.

Stem cell research & therapy

Ullah M,Liu DD,Rai S,Razavi M,Concepcion W,Thakor AS

Published figure using NFkB p65 polyclonal antibody (Product # PA5-16545) in Western Blot

Mon Sep 14 00:00:00 EDT 2020

Anti-inflammatory Effect of Curcuma longa and Allium hookeri Co-treatment via NF-κB and COX-2 Pathways.

Scientific reports

Lee SY,Cho SS,Li Y,Bae CS,Park KM,Park DH

Published figure using NFkB p65 polyclonal antibody (Product # PA5-16545) in Immunofluorescence

Tue Mar 31 00:00:00 EDT 2020

Anti-inflammatory Effect of Curcuma longa and Allium hookeri Co-treatment via NF-κB and COX-2 Pathways.

Scientific reports

Lee SY,Cho SS,Li Y,Bae CS,Park KM,Park DH

Published figure using NFkB p65 polyclonal antibody (Product # PA5-16545) in Immunofluorescence

Tue Mar 31 00:00:00 EDT 2020

Anti-inflammatory Effect of Curcuma longa and Allium hookeri Co-treatment via NF-κB and COX-2 Pathways.

Scientific reports

Lee SY,Cho SS,Li Y,Bae CS,Park KM,Park DH

Published figure using NFkB p65 polyclonal antibody (Product # PA5-16545) in Immunofluorescence

Tue Mar 31 00:00:00 EDT 2020

Pristimerin protects against doxorubicin-induced cardiotoxicity and fibrosis through modulation of Nrf2 and MAPK/NF-kB signaling pathways.

Cancer management and research

El-Agamy DS,El-Harbi KM,Khoshhal S,Ahmed N,Elkablawy MA,Shaaban AA,Abo-Haded HM

PA5-16545 was used in Western Blotting to investigate the impact of pristimerin on doxorubicin-induced cardiotoxic effects.

Wed Mar 30 00:00:00 EDT 2022

Nano hydroxyapatite induces glioma cell apoptosis by suppressing NF-κB signaling pathway.

Experimental and therapeutic medicine

Guo G,Tian A,Lan X,Fu C,Yan Z,Wang C

Published figure using NFkB p65 polyclonal antibody (Product # PA5-16545) in Immunocytochemistry

Wed May 01 00:00:00 EDT 2019

Nano hydroxyapatite induces glioma cell apoptosis by suppressing NF-κB signaling pathway.

Experimental and therapeutic medicine

Guo G,Tian A,Lan X,Fu C,Yan Z,Wang C

Published figure using NFkB p65 polyclonal antibody (Product # PA5-16545) in Immunocytochemistry

Wed May 01 00:00:00 EDT 2019

Mitochondrial Damage Causes Inflammation via cGAS-STING Signaling in Acute Kidney Injury.

Cell reports

Maekawa H,Inoue T,Ouchi H,Jao TM,Inoue R,Nishi H,Fujii R,Ishidate F,Tanaka T,Tanaka Y,Hirokawa N,Nangaku M,Inagi R

PA5-16545 was used in Western Blot to conclude that mitochondrial dysfunction and subsequent activation of the mtDNA-cGAS-STING pathway is a critical regulator of kidney injury.

Tue Oct 29 00:00:00 EDT 2019

Mechanisms of Fasting-Mediated Protection against Renal Injury and Fibrosis Development after Ischemic Acute Kidney Injury.

Biomolecules

Rojas-Morales P,Tapia E,León-Contreras JC,González-Reyes S,Jiménez-Osorio AS,Trujillo J,Pavón N,Granados-Pineda J,Hernández-Pando R,Sánchez-Lozada LG,Osorio-Alonso H,Pedraza-Chaverri J

PA5-16545 was used in Western Blot to investigate the effect of preoperative fasting on rental input and fibrosis development after ischemia-reperfusion injury of the kidney.

Thu Aug 22 00:00:00 EDT 2019

Inhibition of indoleamine 2,3-dioxygenase attenuates endotoxin-mediated tolerance in granulosa cells through kynurenine pathway.

Journal of cellular biochemistry

Dahiya S,Onteru SK,Singh D

PA5-16545 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to investigate the effects of LPS exposure on buffalo granulosa cells.

Mon Jul 01 00:00:00 EDT 2019

Radiation-induced premature cellular senescence involved in glomerular diseases in rats.

Scientific reports

Aratani S,Tagawa M,Nagasaka S,Sakai Y,Shimizu A,Tsuruoka S

Published figure using NFkB p65 polyclonal antibody (Product # PA5-16545) in Immunofluorescence

Wed Nov 14 00:00:00 EST 2018

Polyphenols from Tamarix nilotica: LC⁻ESI-MSⁿ Profiling and In Vivo Antifibrotic Activity.

Molecules (Basel, Switzerland)

Sekkien A,Swilam N,Ebada SS,Esmat A,El-Khatib AH,Linscheid MW,Singab AN

PA5-16545 was used in Immunohistochemistry to analyse the polyphenol profile of the alcohol soluble fraction of aqueous Tamarix nilotica extract.

Mon Jun 11 00:00:00 EDT 2018

Proteome Analysis of Hypoxic Glioblastoma Cells Reveals Sequential Metabolic Adaptation of One-Carbon Metabolic Pathways.

Molecular & cellular proteomics : MCP

Zhang K,Xu P,Sowers JL,Machuca DF,Mirfattah B,Herring J,Tang H,Chen Y,Tian B,Brasier AR,Sowers LC

PA5-16545 was used in Western Blotting to study the dynamic proteome responses of glioblastoma cells exposed to hypoxia via quantification of newly synthesised proteins.

Wed Nov 01 00:00:00 EDT 2017

Ectopic lymphoid structures function as microniches for tumor progenitor cells in hepatocellular carcinoma.

Nature immunology

Finkin S,Yuan D,Stein I,Taniguchi K,Weber A,Unger K,Browning JL,Goossens N,Nakagawa S,Gunasekaran G,Schwartz ME,Kobayashi M,Kumada H,Berger M,Pappo O,Rajewsky K,Hoshida Y,Karin M,Heikenwalder M,Ben-Neriah Y,Pikarsky E

PA5-16545 was used in immunohistochemistry - paraffin section to study hepatocellular carcinoma and ectopic lymphoid structures function as microniches for tumor progenitor cells

Tue Dec 01 00:00:00 EST 2015

3,4-Dihydroxy-benzohydroxamic acid (Didox) suppresses pro-inflammatory profiles and oxidative stress in TLR4-activated RAW264.7 murine macrophages.

Chemico-biological interactions

Matsebatlela TM,Anderson AL,Gallicchio VS,Elford H,Rice CD

PA5-16545 was used in western blot to study the role of 3,4-Dihydroxy-benzohydroxamic acid (Didox) in acute inflammation and oxidative stress

Mon May 25 00:00:00 EDT 2015

Salmonella Engages Host MicroRNAs To Modulate SUMOylation: a New Arsenal for Intracellular Survival.

Molecular and cellular biology

Verma S,Mohapatra G,Ahmad SM,Rana S,Jain S,Khalsa JK,Srikanth CV

PA5-16545 was used in western blot to report that Salmonella enterica serovar Typhimurium modulates host SUMOylation.

Tue Sep 01 00:00:00 EDT 2015

Z-100, extracted from Mycobacterium tuberculosis strain Aoyama B, promotes TNF-α production via nucleotide-binding oligomerization domain containing 2 (Nod2)-dependent NF-κB activation in RAW264.7 cells.

Molecular immunology

Katsunuma K,Yoshinaga K,Ohira Y,Eta R,Sato T,Horii T,Tanaka T,Takei M,Seto K

PA5-16545 was used in immunocytochemistry to evaluated the effects of Z-100 on NF-kB signaling in the murine macrophage cell line RAW264.7

Sun Mar 01 00:00:00 EST 2015

Cardiac-Specific Activation of IKK2 Leads to Defects in Heart Development and Embryonic Lethality.

PloS one

Kraut B,Maier HJ,Kókai E,Fiedler K,Boettger T,Illing A,Kostin S,Walther P,Braun T,Wirth T

PA5-16545 was used in immunohistochemistry to characterize defects in heart development and embryonic lethality due to cardiac-specific activation of IKK2

Mon Jun 13 00:00:00 EDT 2016

Intestinal tumorigenesis initiated by dedifferentiation and acquisition of stem-cell-like properties.

Cell

Schwitalla S,Fingerle AA,Cammareri P,Nebelsiek T,Göktuna SI,Ziegler PK,Canli O,Heijmans J,Huels DJ,Moreaux G,Rupec RA,Gerhard M,Schmid R,Barker N,Clevers H,Lang R,Neumann J,Kirchner T,Taketo MM,van den Brink GR,Sansom OJ,Arkan MC,Greten FR

PA5-16545 was used in immunohistochemistry to study the role of inflammatory signaling in the dedifferentiation and acquisition of stem-like properties associated with intestinal tumorigenesis

Thu Jan 17 00:00:00 EST 2013

Chemopreventive efficacy of green tea drinking against 1,2-dimethyl hydrazine-induced rat colon carcinogenesis.

Cell biochemistry and function

Sadik NA

PA5-16545 was used in immunohistochemistry to study the ability of drinking green tea to prevent against colon carcinogenesis in a rat model

Mon Apr 01 00:00:00 EDT 2013

Surgical removal of endometrioma decreases the NF-kB1 (p50/105) and NF-kB p65 (Rel A) expression in the eutopic endometrium during the implantation window.

Reproductive sciences (Thousand Oaks, Calif.)

Celik O,Celik E,Turkcuoglu I,Yilmaz E,Ulas M,Simsek Y,Karaer A,Celik N,Aydin NE,Ozerol I,Unlu C

PA5-16545 was used in immunohistochemistry to study endometrial levels of NFkB1 and RelA following surgical endometrioma removal

Mon Jul 01 00:00:00 EDT 2013

Nuclear transcription factor-kappa beta-dependent ultrastructural alterations within the placenta and systemic inflammatory activation in pregnant patients with hemolysis, elevated liver functions and low thrombocyte count (HELLP) syndrome: a case-control study.

Hypertension in pregnancy

Simsek Y,Gul M,Celik O,Aydin NE,Arda Düz S,Celik E,Ozerol E,Özerol IH,Tanbek K

PA5-16545 was used in immunohistochemistry to study the potential role of placental NFkB overexpression in the pathophysiology of HELLP syndrome

Thu Aug 01 00:00:00 EDT 2013

Oral epithelial barrier function and the role of nuclear factor kappa-β pathway in the pathogenesis of aphthous ulceration.

The Turkish journal of gastroenterology : the official journal of Turkish Society of Gastroenterology

Günhan Ö,Günal A,Avcı A,Kurgan Ş,Dereci Ö,Kurt B,Celasun B

PA5-16545 was used in immunohistochemistry to study loss of oral epithelial barrier function in the pathogenesis of aphthous ulceration and the involvement of NFkB signaling pathways

Wed Jan 21 00:00:00 EST 2015

Identification of multipath genes differentially expressed in pathway-targeted microarrays in zebrafish infected and surviving spring viremia carp virus (SVCV) suggest preventive drug candidates.

PloS one

Encinas P,Garcia-Valtanen P,Chinchilla B,Gomez-Casado E,Estepa A,Coll J

PA5-16545 was used in immunocytochemistry to use zebrafish to identify factors altered during infection with spring viremia carp virus

Mon Apr 28 00:00:00 EDT 2014

Comparative gene expression profiling identifies common molecular signatures of NF-κB activation in canine and human diffuse large B cell lymphoma (DLBCL).

PloS one

Mudaliar MA,Haggart RD,Miele G,Sellar G,Tan KA,Goodlad JR,Milne E,Vail DM,Kurzman I,Crowther D,Argyle DJ

PA5-16545 was used in western blot to perform a comparative study of the gene expression profiles of canine and human diffuse large B-cell lymphoma with particular reference to the NFkB pathway

Wed Apr 02 00:00:00 EDT 2014

Hepatic activation of IKK/NFκB signaling induces liver fibrosis via macrophage-mediated chronic inflammation.

Hepatology (Baltimore, Md.)

Sunami Y,Leithäuser F,Gul S,Fiedler K,Güldiken N,Espenlaub S,Holzmann KH,Hipp N,Sindrilaru A,Luedde T,Baumann B,Wissel S,Kreppel F,Schneider M,Scharffetter-Kochanek K,Kochanek S,Strnad P,Wirth T

PA5-16545 was used in immunohistochemistry to study the role of chronic inflammation mediated by macrophages in the development of fibrosis following activation of hepatic IKK/NFkB

Sat Sep 01 00:00:00 EDT 2012

Effect of Si-Miao-Yong-An on the stability of atherosclerotic plaque in a diet-induced rabbit model.

Journal of ethnopharmacology

Peng L,Li M,Xu YZ,Zhang GY,Yang C,Zhou YN,Li LJ,Zhang JP

PA5-16545 was used in immunohistochemistry to study the ability of the Chinese herbal remedy Si-Miao-Yong-An to increase atherosclerotic plaque stability

Thu Aug 30 00:00:00 EDT 2012

Calpastatin upregulation in Mycoplasma hyorhinis-infected cells is promoted by the mycoplasma lipoproteins via the NF-κB pathway.

Cellular microbiology

Elkind E,Vaisid T,Kornspan JD,Barnoy S,Rottem S,Kosower NS

PA5-16545 was used in immunocytochemistry and western blot to study the mechanism by which mycoplasma lipoproteins upregulate calpastatin in Mycoplasma hyorhinis-infected cells

Fri Jun 01 00:00:00 EDT 2012

Potential role of p38-mitogene-activated protein kinase and nuclear factor-kappa B expression in testicular dysfunction associated with varicocele: an experimental study.

Andrologia

Simsek A,Ozbek E,Ilbey YO,Cekmen M,Somay A,Tasci AI

PA5-16545 was used in immunohistochemistry to study the roles of p38-MAPK and NFkB in a rat model of testicular dysfunction

Tue May 01 00:00:00 EDT 2012

Extensive fibrin accumulation and accompanying epithelial changes in the pathogenesis of ligneous mucosal disease (ligneous periodontitis).

The American Journal of dermatopathology

Günhan Ö,Avci A,Dereci Ö,Akgün S,Celasun B

PA5-16545 was used in immunohistochemistry to study the pathogenesis of ligneous peridontitis and the role played by fibrin accumulation

Wed Feb 01 00:00:00 EST 2012

Hsp72 overexpression accelerates the recovery from caerulein-induced pancreatitis.

PloS one

Lunova M,Zizer E,Kucukoglu O,Schwarz C,Dillmann WH,Wagner M,Strnad P

PA5-16545 was used in immunohistochemistry and western blot to study the ability of transgenic overexpression of HSP72 to speed recovery in a murine model of pancreatitis

Tue Mar 19 00:00:00 EDT 2013

Expression of nuclear factor kappa B and survivin in psoriasis.

ISRN dermatology

Gunduz K,Temiz P,Gencoglan G,Inanir I,Catalkaya A

PA5-16545 was used in immunohistochemistry to perform a comparative study of NFkB and survivin expression in normal and psoriatic epidermis

Wed Dec 12 00:00:00 EST 2012

Pulmonary cryptosporidiosis: role of COX2 and NF-kB.

APMIS : acta pathologica, microbiologica, et immunologica Scandinavica

Asaad NY,Sadek GS

PA5-16545 was used in immunohistochemistry - paraffin section to test if Cryptosporidium-induced changes in the lungs are mediated through the activation of COX-2 and NF-kB

Sun Oct 01 00:00:00 EDT 2006

Bioinformatics

Protein Aliases: avian reticuloendotheliosis viral (v-rel) oncogene homolog A; MGC131774; NF KB; nf-kappa-b p65; NF-kappa-B p65delta3; NF-kappa-B transcription factor p65; NF-kappaB transcription factor p65 subunit; NF-kB p65; NFkappaB p65; nfkb rela; NFkB-p50; NFKB1; nuclear factor; nuclear factor kappa b; nuclear factor kappa B subunit p65; Nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3; p65 NF kappaB; p65 NF-kappa B; p65 NFkB; Putative transcription factor p65 homolog; REL A; Transcription factor p65; v-rel avian reticuloendotheliosis viral oncogene homolog A; v-rel reticuloendotheliosis viral oncogene homolog A; v-rel reticuloendotheliosis viral oncogene homolog A, nuclear factor of kappa light polypeptide gene enhancer in B-cells 3, p65; XRel1

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Gene Aliases: I79_021812; NFkB; NFKB3; p65; rel1; RELA; rela-a; XELAEV_18022301mg; Xrel1; xrela

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UniProt ID: (Human) Q04206, (Mouse) Q04207, (Xenopus) Q04865

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Entrez Gene ID: (Human) 5970, (Dog) 483729, (Bovine) 508233, (Pig) 100135665, (Mouse) 19697, (Chinese hamster) 100763542, (Rat) 309165, (Xenopus) 394353

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Function(s)

RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II distal enhancer sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding transcriptional activator activity, RNA polymerase II distal enhancer sequence-specific binding DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding protein binding transcription factor binding protein kinase binding chromatin DNA binding ubiquitin protein ligase binding protein complex binding activating transcription factor binding phosphate ion binding identical protein binding protein homodimerization activity actinin binding histone deacetylase binding transcription regulatory region DNA binding protein heterodimerization activity protein N-terminus binding NF-kappaB binding repressing transcription factor binding ankyrin repeat binding enzyme binding sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding Rel homology transcription factor

Process(es)

Performance Guarantee

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NFkB p65 Polyclonal Antibody

NFkB p65 Antibody (PA5-16545) in ICC/IF

Product Details

PA5-16545

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

CUT&RUN (C&R)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

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