Invitrogen

NFkB p65 Monoclonal Antibody (572)

NFkB p65 Antibody in Immunocytochemistry (ICC/IF)

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NFkB p65 Antibody (436700) in ICC/IF

NF-kappa-B (p65) Clone: 572 Product # 436700. Immunoflorescence of 4% PFA-fixed Hela cells (A) NF-kappa-B (P65) Clone: 572 Product # 436700 shown in green, Alexa Fluor® 488 secondary antibody. Nuclei are shown in blue with DAPI. (B) No primary antibody (negative control), Alexa Fluor® 488 secondary antibody, and Nuclei are shown in blue with DAPI.

NFkB p65 Antibody in Immunoprecipitation (IP)

NFkB p65 Antibody in Immunohistochemistry (IHC)

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Product Details

436700

Applications

Tested Dilution

Publications

Western Blot (WB)

2-3 µg/mL

View 6 publications 6 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

Assay-dependent

View 4 publications 4 publications

Immunoprecipitation (IP)

Assay-dependent

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Bovine, Dog, Horse, Human, Mouse, Non-human primate, Rhesus monkey, Rat

Published species

Dog, Horse, Human, Mouse, Rat

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

572

Immunogen

Recombinant protein derived from the N-terminus region of human NFkB (p65) protein

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10374748

Target Information

Nuclear factor kB p65 (NF-kB p65) is encoded by the RELA gene and is present on chromosome 11 in humans. NF-kB P65 is also known as RelA (v-rel avian reticuloendotheliosis viral oncogene homolog A) and belongs to the Rel family of proteins. It is one of the two subunits of NF-kB (Nuclear factor kappa-light-chain-enhancer of activated B cells) that heterodimerizes with the other subunits p50 or p52. However, binding of TNF alpha to its cognate receptor phosphorylates IKK which in turn phosphorylates I kB allowing proteasomal degradation of I kB. It specifically plays a key role in transcription of immunoglobulin k (kappa) gene in mature B-lymphoid cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Anethole Pretreatment Modulates Cerebral Ischemia/Reperfusion: The Role of JNK, p38, MMP-2 and MMP-9 Pathways.

Pharmaceuticals (Basel, Switzerland)

Younis NS,Mohamed ME

436700 was used in Immunohistochemistry (Paraffin) to reveal the neuroprotective ability of anethole against cerebral ischemia/reperfusion in rats.

Wed Mar 15 00:00:00 EDT 2023

The role of PKM2 nuclear translocation in the constant activation of the NF-κB signaling pathway in cancer-associated fibroblasts.

Cell death & disease

Gu J,Li X,Zhao L,Yang Y,Xue C,Gao Y,Li J,Han Q,Sun Z,Bai C,Zhao RC

Published figure using NFkB p65 monoclonal antibody (Product # 436700) in Western Blot

Wed Mar 17 00:00:00 EDT 2021

The role of PKM2 nuclear translocation in the constant activation of the NF-κB signaling pathway in cancer-associated fibroblasts.

Cell death & disease

Gu J,Li X,Zhao L,Yang Y,Xue C,Gao Y,Li J,Han Q,Sun Z,Bai C,Zhao RC

Published figure using NFkB p65 monoclonal antibody (Product # 436700) in Western Blot

Wed Mar 17 00:00:00 EDT 2021

The role of PKM2 nuclear translocation in the constant activation of the NF-κB signaling pathway in cancer-associated fibroblasts.

Cell death & disease

Gu J,Li X,Zhao L,Yang Y,Xue C,Gao Y,Li J,Han Q,Sun Z,Bai C,Zhao RC

Published figure using NFkB p65 monoclonal antibody (Product # 436700) in Western Blot

Wed Mar 17 00:00:00 EDT 2021

The role of PKM2 nuclear translocation in the constant activation of the NF-κB signaling pathway in cancer-associated fibroblasts.

Cell death & disease

Gu J,Li X,Zhao L,Yang Y,Xue C,Gao Y,Li J,Han Q,Sun Z,Bai C,Zhao RC

Published figure using NFkB p65 monoclonal antibody (Product # 436700) in Western Blot

Wed Mar 17 00:00:00 EDT 2021

Hsp70 and NF-kB Mediated Control of Innate Inflammatory Responses in a Canine Macrophage Cell Line.

International journal of molecular sciences

Lyu Q,Wawrzyniuk M,Rutten VPMG,van Eden W,Sijts AJAM,Broere F

436700 was used in Western Blotting to reveal that Hsp70 may regulate inflammatory responses through NF-κB activation and cytokine expression in canine macrophages.

Fri Sep 04 00:00:00 EDT 2020

Hsp70 and NF-kB Mediated Control of Innate Inflammatory Responses in a Canine Macrophage Cell Line.

International journal of molecular sciences

Lyu Q,Wawrzyniuk M,Rutten VPMG,van Eden W,Sijts AJAM,Broere F

436700 was used in Western Blotting to reveal that Hsp70 may regulate inflammatory responses through NF-κB activation and cytokine expression in canine macrophages.

Fri Sep 04 00:00:00 EDT 2020

A novel mechanism for the protection of embryonic stem cell derived tenocytes from inflammatory cytokine interleukin 1 beta.

Scientific reports

McClellan A,Evans R,Sze C,Kan S,Paterson Y,Guest D

436700 was used in Immunocytochemistry to demonstrate that in tenocytes IL-1Β increases the expression of matrix metalloproteinases, tenascin-C and Sox9 and decreases the expression of scleraxis and cartilage oligomeric matrix protein.

Tue Feb 26 00:00:00 EST 2019

Plastin 3 influences bone homeostasis through regulation of osteoclast activity.

Human molecular genetics

Neugebauer J,Heilig J,Hosseinibarkooie S,Ross BC,Mendoza-Ferreira N,Nolte F,Peters M,Hölker I,Hupperich K,Tschanz T,Grysko V,Zaucke F,Niehoff A,Wirth B

436700 was used in Western Blot to show that regulation of osteoclastogenesis and bone remodeling via the PLS3-NKRF-NFκB-NFATC1 axis unveils a novel possibility to counteract osteoporosis.

Sat Dec 15 00:00:00 EST 2018

Plastin 3 influences bone homeostasis through regulation of osteoclast activity.

Human molecular genetics

Neugebauer J,Heilig J,Hosseinibarkooie S,Ross BC,Mendoza-Ferreira N,Nolte F,Peters M,Hölker I,Hupperich K,Tschanz T,Grysko V,Zaucke F,Niehoff A,Wirth B

436700 was used in Western Blot to show that regulation of osteoclastogenesis and bone remodeling via the PLS3-NKRF-NFκB-NFATC1 axis unveils a novel possibility to counteract osteoporosis.

Sat Dec 15 00:00:00 EST 2018

Memory and PTPIP51--a new protein in hippocampus and cerebellum.

Molecular and cellular neurosciences

Brobeil A,Viard M,Petri MK,Steger K,Tag C,Wimmer M

436700 was used in proximity ligation assay to study the role of PTPIP51 in memory processing.

Thu Jan 01 00:00:00 EST 2015

Possible Mechanisms of Di(2-ethylhexyl) Phthalate-Induced MMP-2 and MMP-9 Expression in A7r5 Rat Vascular Smooth Muscle Cells.

International journal of molecular sciences

Shih MF,Pan KH,Cherng JY

Published figure using NFkB p65 monoclonal antibody (Product # 436700) in Western Blot

Fri Dec 04 00:00:00 EST 2015

Possible Mechanisms of Di(2-ethylhexyl) Phthalate-Induced MMP-2 and MMP-9 Expression in A7r5 Rat Vascular Smooth Muscle Cells.

International journal of molecular sciences

Shih MF,Pan KH,Cherng JY

Published figure using NFkB p65 monoclonal antibody (Product # 436700) in Western Blot

Fri Dec 04 00:00:00 EST 2015

Co-expression of β-arrestin1 and NF-кB is associated with cancer progression and poor prognosis in lung adenocarcinoma.

Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine

Yu J,Wang L,Zhang T,Shen H,Dong W,Ni Y,Du J

436700 was used in immunohistochemistry (paraffin) to correlate β-arrestin1 and NF-κB expression and the clinicopathological characteristics in lung adenocarcinoma.

Sat Aug 01 00:00:00 EDT 2015

Targeting IκB proteins for HIV latency activation: the role of individual IκB and NF-κB proteins.

Journal of virology

Fernandez G,Zaikos TD,Khan SZ,Jacobi AM,Behlke MA,Zeichner SL

Published figure using NFkB p65 monoclonal antibody (Product # 436700) in Western Blot

Mon Apr 01 00:00:00 EDT 2013

Osteopontin enhances HIV replication and is increased in the brain and cerebrospinal fluid of HIV-infected individuals.

Journal of neurovirology

Brown A,Islam T,Adams R,Nerle S,Kamara M,Eger C,Marder K,Cohen B,Schifitto G,McArthur JC,Sacktor N,Pardo CA

Published figure using NFkB p65 monoclonal antibody (Product # 436700) in Immunofluorescence

Mon Aug 01 00:00:00 EDT 2011

Osteopontin enhances HIV replication and is increased in the brain and cerebrospinal fluid of HIV-infected individuals.

Journal of neurovirology

Brown A,Islam T,Adams R,Nerle S,Kamara M,Eger C,Marder K,Cohen B,Schifitto G,McArthur JC,Sacktor N,Pardo CA

Published figure using NFkB p65 monoclonal antibody (Product # 436700) in Immunofluorescence

Mon Aug 01 00:00:00 EDT 2011

Tumor protein p63/nuclear factor κB feedback loop in regulation of cell death.

The Journal of biological chemistry

Sen T,Sen N,Huang Y,Sinha D,Luo ZG,Ratovitski EA,Sidransky D

Published figure using NFkB p65 monoclonal antibody (Product # 436700) in Western Blot

Fri Dec 16 00:00:00 EST 2011

Bioinformatics

Protein Aliases: avian reticuloendotheliosis viral (v-rel) oncogene homolog A; MGC131774; NF KB; nf-kappa-b p65; NF-kappa-B p65delta3; NF-kappa-B transcription factor p65; NF-kappaB transcription factor p65 subunit; NF-kB p65; NFkappaB p65; nfkb rela; NFkB-p50; NFKB1; nuclear factor; nuclear factor kappa b; nuclear factor kappa B subunit p65; Nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3; p65 NF kappaB; p65 NF-kappa B; p65 NFkB; REL A; Transcription factor p65; v-rel avian reticuloendotheliosis viral oncogene homolog A; v-rel reticuloendotheliosis viral oncogene homolog A; v-rel reticuloendotheliosis viral oncogene homolog A, nuclear factor of kappa light polypeptide gene enhancer in B-cells 3, p65

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Gene Aliases: NFkB; NFKB3; p65; RELA

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UniProt ID: (Human) Q04206, (Mouse) Q04207

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Entrez Gene ID: (Human) 5970, (Dog) 483729, (Horse) 100057483, (Bovine) 508233, (Rhesus monkey) 715919, (Mouse) 19697, (Rat) 309165

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Function(s)

RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II distal enhancer sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding transcriptional activator activity, RNA polymerase II distal enhancer sequence-specific binding DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding protein binding transcription factor binding protein kinase binding chromatin DNA binding ubiquitin protein ligase binding protein complex binding activating transcription factor binding phosphate ion binding identical protein binding protein homodimerization activity actinin binding histone deacetylase binding transcription regulatory region DNA binding protein heterodimerization activity protein N-terminus binding NF-kappaB binding repressing transcription factor binding ankyrin repeat binding enzyme binding sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding Rel homology transcription factor

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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NFkB p65 Monoclonal Antibody (572)

NFkB p65 Antibody (436700) in ICC/IF

Product Details

436700

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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