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NG2 Antibody (OSN00019W-100UL) in IHC (P)
IHC-P on paraffin sections of mouse olfactory bulbs. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 mL 4% PFA being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB ... View More
Product Details
OSN00019W-100UL
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Not Applicable
Host/Isotype
Rabbit
/ Ig
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide from extracellular domain of mouse NG2 conjugated to blue carrier protein was used as the antigen
Conjugate
Form
Lyophilized
Concentration
Conc. Not Determined
Storage buffer
whole serum
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for added stability.
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
Specificity of this antibody: NG2.
Target Information
A human melanoma-associated chondroitin sulfate proteoglycan plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. CSPG4 represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: AN2 proteoglycan; AN2/NG2 proteoglycan; Chondroitin sulfate proteoglycan 4; Chondroitin sulfate proteoglycan NG2; HMW MAA; MEL CSPG; nerve/glial antigen 2; Proteoglycan AN2
Gene Aliases: 4732461B14Rik; AN2; Cspg4; Kiaa4232; NG2
UniProt ID: (Mouse) Q8VHY0
Entrez Gene ID: (Mouse) 121021
activation of MAPK activity
angiogenesis
inflammatory response
signal transduction
transmembrane receptor protein tyrosine kinase signaling pathway
multicellular organism development
cell proliferation
glial cell migration
negative regulation of neuron projection development
neuron remodeling
cell differentiation
intracellular signal transduction
tissue remodeling
positive regulation of peptidyl-tyrosine phosphorylation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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