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Invitrogen
NG2 Monoclonal Antibody (546930)
Advanced Verification
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 7
NG2 Antibody (MA5-24247) in ICC/IF
Immunofluorescence analysis of NG2 was performed using 70% confluent log phase A-375 cells. The cells were fixed with 4% Paraformaldehyde for 10 Minutes, permeabilized with 0.1% Triton™ X-100 for 10 Minutes, and blocked with 2% BSA for 10 Minutes at room temperature. The cells were labeled with Anti-NG2 Monoclonal Antibody (546930) (Product # MA5-24247) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled Goat anti-Rat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 (Product # A-11006, 1:2,000 dilut... View More
Product Details
MA5-24247
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Rat
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
546930
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse NG2/MCSP Leu1587-Ser2229
Conjugate
Form
Lyophilized
Concentration
0.5 mg/mL
Purification
Protein A/G
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2606388
Product Specific Information
In direct ELISAs, no cross-reactivity with recombinant human NG2/MCSP/CSPG4 is observed.
Reconstitute in sterile PBS to a final concentration of 0.5 mg/mL.
Target Information
A human melanoma-associated chondroitin sulfate proteoglycan plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. CSPG4 represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: AN2 proteoglycan; AN2/NG2 proteoglycan; Chondroitin sulfate proteoglycan 4; Chondroitin sulfate proteoglycan NG2; HMW MAA; MEL CSPG; nerve/glial antigen 2; Proteoglycan AN2
Gene Aliases: 4732461B14Rik; AN2; Cspg4; Kiaa4232; NG2
UniProt ID: (Mouse) Q8VHY0
Entrez Gene ID: (Mouse) 121021
activation of MAPK activity
angiogenesis
inflammatory response
signal transduction
transmembrane receptor protein tyrosine kinase signaling pathway
multicellular organism development
cell proliferation
glial cell migration
negative regulation of neuron projection development
neuron remodeling
cell differentiation
intracellular signal transduction
tissue remodeling
positive regulation of peptidyl-tyrosine phosphorylation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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