Invitrogen

NG2 Monoclonal Antibody (D120.43/D4.11/N143.8/N109.6)

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Published Figures (10)

NG2 Antibody in Western Blot, Immunocytochemistry, Neutralization (WB, ICC/IF, Neu)

FIGURE: 1 / 10

NG2 Antibody (37-2700) in WB, ICC/IF, Neu

Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism 2016 - Published figure using NG2 monoclonal antibody (Product # 37-2700) in Western Blot, Immunocytochemistry and Neutralization

NG2 Antibody in Immunohistochemistry (Frozen) (IHC (F))

NG2 Antibody in Immunohistochemistry, Immunomicroscopy (IHC, IM)

NG2 Antibody in Immunohistochemistry (IHC)

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Product Details

37-2700

Applications

Tested Dilution

Publications

Western Blot (WB)

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 6 publications 6 publications

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

View 1 publication 1 publication

Immunohistochemistry - Free Floating (IHC (Free))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

Flow Cytometry (Flow)

View 2 publications 2 publications

ELISA (ELISA)

Assay-dependent

Immunoprecipitation (IP)

Assay-dependent

Neutralization (Neu)

View 1 publication 1 publication

Immunomicroscopy (IM)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Rat

Published species

Mouse, Rat

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

D120.43/D4.11/N143.8/N109.6

Immunogen

Purified rat NG2 protein.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533307

Target Information

A human melanoma-associated chondroitin sulfate proteoglycan plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. CSPG4 represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Discovery of Hippo signaling as a regulator of CSPG4 expression and as a therapeutic target for Clostridioides difficile disease.

PLoS pathogens

Larabee JL,Doyle DA,Ahmed UKB,Shadid TM,Sharp RR,Jones KL,Kim YM,Li S,Ballard JD

Published figure using NG2 monoclonal antibody (Product # 37-2700) in Flow Cytometry

Wed Mar 01 00:00:00 EST 2023

Endocannabinoid signaling in oligodendroglia.

Glia

Molina-Holgado E,Esteban PF,Arevalo-Martin Á,Moreno-Luna R,Molina-Holgado F,Garcia-Ovejero D

37-2700 was used in ELISA, Immunohistochemistry (Frozen), Immunoprecipitation to evaluate the evidence showing that oligodendrocytes express a full endocannabinoid signaling machinery involved in physiological oligodendrocyte functions that can be therapeutically exploited to promote remyelination in central nervous system pathologies.

Sun Jan 01 00:00:00 EST 2023

TUBB4A mutations result in both glial and neuronal degeneration in an H-ABC leukodystrophy mouse model.

eLife

Sase S,Almad AA,Boecker CA,Guedes-Dias P,Li JJ,Takanohashi A,Patel A,McCaffrey T,Patel H,Sirdeshpande D,Curiel J,Shih-Hwa Liu J,Padiath Q,Holzbaur EL,Scherer SS,Vanderver A

37-2700 was used in Immunohistochemistry to show decreased survival and dysfunction in microtubule dynamics in neurons from Tubb4aD249N/D249N mice.

Thu May 28 00:00:00 EDT 2020

Resistance to retinopathy development in obese, diabetic and hypertensive ZSF1 rats: an exciting model to identify protective genes.

Scientific reports

Caolo V,Roblain Q,Lecomte J,Carai P,Peters L,Cuijpers I,Robinson EL,Derks K,Sergeys J,Noël A,Jones EAV,Moons L,Heymans S

37-2700 was used in Immunohistochemistry-immunofluorescence to investigate whether obese, 42-weeks old ZSF1 rats are a suitable model to study diabetic retinopathy.

Thu Aug 09 00:00:00 EDT 2018

CD200 restrains macrophage attack on oligodendrocyte precursors via toll-like receptor 4 downregulation.

Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism

Hayakawa K,Pham LD,Seo JH,Miyamoto N,Maki T,Terasaki Y,Sakadžić S,Boas D,van Leyen K,Waeber C,Kim KW,Arai K,Lo EH

37-2700 was used in flow cytometry to elucidate how CD200 restrains macrophage attacks on oligodendrocyte precursors by tollike receptor 4

Fri Apr 01 00:00:00 EDT 2016

CD200 restrains macrophage attack on oligodendrocyte precursors via toll-like receptor 4 downregulation.

Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism

Hayakawa K,Pham LD,Seo JH,Miyamoto N,Maki T,Terasaki Y,Sakadžić S,Boas D,van Leyen K,Waeber C,Kim KW,Arai K,Lo EH

37-2700 was used in flow cytometry to elucidate how CD200 restrains macrophage attacks on oligodendrocyte precursors by tollike receptor 4

Fri Apr 01 00:00:00 EDT 2016

CD200 restrains macrophage attack on oligodendrocyte precursors via toll-like receptor 4 downregulation.

Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism

Hayakawa K,Pham LD,Seo JH,Miyamoto N,Maki T,Terasaki Y,Sakadžić S,Boas D,van Leyen K,Waeber C,Kim KW,Arai K,Lo EH

37-2700 was used in flow cytometry to elucidate how CD200 restrains macrophage attacks on oligodendrocyte precursors by tollike receptor 4

Fri Apr 01 00:00:00 EDT 2016

CD200 restrains macrophage attack on oligodendrocyte precursors via toll-like receptor 4 downregulation.

Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism

Hayakawa K,Pham LD,Seo JH,Miyamoto N,Maki T,Terasaki Y,Sakadžić S,Boas D,van Leyen K,Waeber C,Kim KW,Arai K,Lo EH

37-2700 was used in flow cytometry to elucidate how CD200 restrains macrophage attacks on oligodendrocyte precursors by tollike receptor 4

Fri Apr 01 00:00:00 EDT 2016

Perivascular-derived stem cells with neural crest characteristics are involved in tendon repair.

Stem cells and development

Xu W,Sun Y,Zhang J,Xu K,Pan L,He L,Song Y,Njunge L,Xu Z,Chiang MY,Sung KL,Chuong CM,Yang L

37-2700 was used in Immunocytochemistry-immunoflourescence to demonstrate that rat tendons contain a unique subpopulation of cells exhibiting stem cell characteristics.

Wed Apr 01 00:00:00 EDT 2015

Spinal cord injury induces a long-lasting upregulation of interleukin-1β in astrocytes around the central canal.

Neuroscience

Paniagua-Torija B,Arevalo-Martin A,Molina-Holgado E,Molina-Holgado F,Garcia-Ovejero D

37-2700 was used in immunohistochemistry - free floating to assess IL-1beta expression in periependymal astrocytes after injury

Thu Jan 22 00:00:00 EST 2015

Phenotype overlap in glial cell populations: astroglia, oligodendroglia and NG-2(+) cells.

Frontiers in neuroanatomy

Alghamdi B,Fern R

37-2700 was used in immunohistochemistry to study the phenotype overlap of different glial cell populations of NG-2 (+), astroglia, and oligodendroglia cells

Wed Jun 24 00:00:00 EDT 2015

Phenotype overlap in glial cell populations: astroglia, oligodendroglia and NG-2(+) cells.

Frontiers in neuroanatomy

Alghamdi B,Fern R

37-2700 was used in immunohistochemistry to study the phenotype overlap of different glial cell populations of NG-2 (+), astroglia, and oligodendroglia cells

Wed Jun 24 00:00:00 EDT 2015

Spatio-temporal expression pattern of frizzled receptors after contusive spinal cord injury in adult rats.

PloS one

Gonzalez P,Fernandez-Martos CM,Gonzalez-Fernandez C,Arenas E,Rodriguez FJ

37-2700 was used in immunohistochemistry - frozen section to study the expression of Frizzled receptors in the damaged spinal cord.

Mon Jun 03 00:00:00 EDT 2013

Chemokine expression in the white matter spinal cord precursor niche after force-defined spinal cord contusion injuries in adult rats.

Glia

Knerlich-Lukoschus F,von der Ropp-Brenner B,Lucius R,Mehdorn HM,Held-Feindt J

Tue Jun 01 00:00:00 EDT 2010

Subcellular localization of transporters along the rat blood-brain barrier and blood-cerebral-spinal fluid barrier by in vivo biotinylation.

Neuroscience

Roberts LM,Black DS,Raman C,Woodford K,Zhou M,Haggerty JE,Yan AT,Cwirla SE,Grindstaff KK

37-2700 was used in immunohistochemistry to study the luminal/abluminal localization of a number of blood-brain barrier transporters expressed by endothelial cells

Wed Aug 13 00:00:00 EDT 2008

Bioinformatics

Protein Aliases: Chondroitin sulfate proteoglycan 4; chondroitin sulfate proteoglycan 4 (melanoma-associated); Chondroitin sulfate proteoglycan NG2; HMW MAA; HSN tumor-specific antigen; MEL CSPG; Melanoma chondroitin sulfate proteoglycan; Melanoma-associated chondroitin sulfate proteoglycan; membrane-spanning proteoglycan NG2

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Gene Aliases: CSPG4; HMW-MAA; MCSP; MCSPG; MEL-CSPG; MSK16; NG2

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UniProt ID: (Human) Q6UVK1, (Rat) Q00657

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Entrez Gene ID: (Human) 1464, (Rat) 81651

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Function(s)

signal transducer activity protein kinase binding collagen binding

Process(es)

activation of MAPK activity angiogenesis transmembrane receptor protein tyrosine kinase signaling pathway cell proliferation glial cell migration cell migration glycosaminoglycan metabolic process chondroitin sulfate biosynthetic process chondroitin sulfate catabolic process dermatan sulfate biosynthetic process intracellular signal transduction tissue remodeling positive regulation of peptidyl-tyrosine phosphorylation inflammatory response signal transduction response to lithium ion negative regulation of neuron projection development neuron remodeling

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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NG2 Monoclonal Antibody (D120.43/D4.11/N143.8/N109.6)

NG2 Antibody (37-2700) in WB, ICC/IF, Neu

Product Details

37-2700

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Frozen) (IHC (F))

Immunohistochemistry - Free Floating (IHC (Free))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

Immunoprecipitation (IP)

Neutralization (Neu)

Immunomicroscopy (IM)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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