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NIT2 Antibody (A305-782A) in WB
Detection of human NIT2 by western blot. Samples: Whole cell lysate (15 µg) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-NIT2 antibody (A305-782A lot 1) used for WB at 0.1 mg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.
Product Details
A305-782A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A region between residue 226 and 276 of human Omega-amidase NIT2.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
phosphate, tris citrate
Contains
0.09% sodium azide
Storage conditions
4° C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Predicted reactivity by homology: Orangutan.
Target Information
Has a omega-amidase activity. The role of omega-amidase is to remove potentially toxic intermediates by converting alpha-ketoglutaramate and alpha-ketosuccinamate to biologically useful alpha-ketoglutarate and oxaloacetate, respectively. Overexpression decreases the colony-forming capacity of cultured cells by arresting cells in the G2 phase of the cell cycle.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: epididymis secretory sperm binding protein Li 8a; Nit protein 2; nitrilase family, member 2; Nitrilase homolog 2; Omega-amidase NIT2
Gene Aliases: CUA002; HEL-S-8a; NIT2
UniProt ID: (Human) Q9NQR4
Entrez Gene ID: (Human) 56954
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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