Proteintech

NKCC2 Polyclonal Antibody

NKCC2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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NKCC2 Antibody (18970-1-AP) in IHC (P)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue slide using (Product # 18970-1-AP) NKCC2 antibody at dilution of 1:4,000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

NKCC2 Antibody in Immunohistochemistry (Frozen) (IHC (F))

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Product Details

18970-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 4 publications 4 publications

Immunohistochemistry (IHC)

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

Immunocytochemistry (ICC/IF)

1:20-1:200

View 17 publications 17 publications

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

NKCC2 Fusion Protein Ag5325 (1-176 aa encoded by BC040138)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.14 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MSLNNSSNV FLDSVPSNTN RFQVSVINEN HESSAAADDN TDPPHYEETS FGDEAQKRLR ISFRPGNQEC YDNFLQSGET AKTDASFHAY DSHTNTYYLQ TFGHNTMDAV PKIEYYRNTG SISGPKVNRP SLLEIHEQLA KNVAVTPSSA DRVANGDGIP GDEQAENKED DQAGVVK (1-176 aa encoded by BC040138)

Target Information

The sodium-potassium-chloride cotransporter isoform 2 is kidney-specific and is found on the apical membrane of the thick ascending limb of Henle's loop and the macula densa. It accounts for most of the NaCl resorption with the stoichiometry of 1Na:1K:2Cl and is sensitive to such diuretics as furosemide and bumetanide. Some Bartter-like syndromes result from defects in this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Generation and characterization of an inducible renal proximal tubule-specific CreERT2 mouse.

Frontiers in cell and developmental biology

Liang S,Wang Y,Kang M,Deng J,Chen L,Hong X,Hou FF,Zhang F

Tue May 23 00:00:00 EDT 2023

Mechanism of interdigitation formation at apical boundary of MDCK cell.

iScience

Miyazaki S,Otani T,Sugihara K,Fujimori T,Furuse M,Miura T

Fri May 19 00:00:00 EDT 2023

Activated SOX9+ renal epithelial cells promote kidney repair through secreting factors.

Cell proliferation

Nie H,Zhao Z,Zhou D,Li D,Wang Y,Ma Y,Liu X,Zuo W

Sat Apr 01 00:00:00 EDT 2023

WT1⁺ glomerular parietal epithelial progenitors promote renal proximal tubule regeneration after severe acute kidney injury.

Theranostics

Hong X,Nie H,Deng J,Liang S,Chen L,Li J,Gong S,Wang G,Zuo W,Hou F,Zhang F

Mon Mar 20 00:00:00 EDT 2023

Tubular-specific CDK12 knockout causes a defect in urine concentration due to premature cleavage of the slc12a1 gene.

Molecular therapy : the journal of the American Society of Gene Therapy

Wang B,Wang Y,Wen Y,Zhang YL,Ni WJ,Tang TT,Cao JY,Yin Q,Jiang W,Yin D,Li ZL,Lv LL,Liu BC

Wed Oct 05 00:00:00 EDT 2022

Tubular-specific CDK12 knockout causes a defect in urine concentration due to premature cleavage of the slc12a1 gene.

Molecular therapy : the journal of the American Society of Gene Therapy

Wang B,Wang Y,Wen Y,Zhang YL,Ni WJ,Tang TT,Cao JY,Yin Q,Jiang W,Yin D,Li ZL,Lv LL,Liu BC

Wed Oct 05 00:00:00 EDT 2022

Tubular-specific CDK12 knockout causes a defect in urine concentration due to premature cleavage of the slc12a1 gene.

Molecular therapy : the journal of the American Society of Gene Therapy

Wang B,Wang Y,Wen Y,Zhang YL,Ni WJ,Tang TT,Cao JY,Yin Q,Jiang W,Yin D,Li ZL,Lv LL,Liu BC

Wed Oct 05 00:00:00 EDT 2022

Tubular-specific CDK12 knockout causes a defect in urine concentration due to premature cleavage of the slc12a1 gene.

Molecular therapy : the journal of the American Society of Gene Therapy

Wang B,Wang Y,Wen Y,Zhang YL,Ni WJ,Tang TT,Cao JY,Yin Q,Jiang W,Yin D,Li ZL,Lv LL,Liu BC

Wed Oct 05 00:00:00 EDT 2022

Gene expression profile and injury sites in mice treated with Shiga toxin 2 and lipopolysaccharide as a Shiga toxin-associated hemolytic uremic syndrome model.

Physiological genomics

Kume Y,Go H,Maeda R,Suyama K,Mori T,Kawasaki Y,Hashimoto K,Hosoya M

Sun May 01 00:00:00 EDT 2022

Enhanced metanephric specification to functional proximal tubule enables toxicity screening and infectious disease modelling in kidney organoids.

bioRxiv : the preprint server for biology

Vanslambrouck JM,Wilson SB,Tan KS,Groenewegen E,Rudraraju R,Neil J,Lawlor KT,Mah S,Scurr M,Howden SE,Subbarao K,Little MH

Fri May 27 00:00:00 EDT 2022

Enhanced metanephric specification to functional proximal tubule enables toxicity screening and infectious disease modelling in kidney organoids.

Nature communications

Vanslambrouck JM,Wilson SB,Tan KS,Groenewegen E,Rudraraju R,Neil J,Lawlor KT,Mah S,Scurr M,Howden SE,Subbarao K,Little MH

Sat Oct 08 00:00:00 EDT 2022

Mechanism of Diuresis and Natriuresis by Cannabinoids: Evidence for Inhibition of Na⁺-K⁺-ATPase in Mouse Kidney Thick Ascending Limb Tubules.

The Journal of pharmacology and experimental therapeutics

Ritter JK,Ahmad A,Mummalaneni S,Daneva Z,Dempsey SK,Li N,Li PL,Lyall V

Fri Jan 01 00:00:00 EST 2021

Pregnane X receptor (PXR) protects against cisplatin-induced acute kidney injury in mice.

Biochimica et biophysica acta. Molecular basis of disease

Luan Z,Wei Y,Huo X,Sun X,Zhang C,Ming W,Luo Z,Du C,Li Y,Xu H,Lu H,Zheng F,Guan Y,Zhang X

Mon Mar 01 00:00:00 EST 2021

Plasticity of distal nephron epithelia from human kidney organoids enables the induction of ureteric tip and stalk.

Cell stem cell

Howden SE,Wilson SB,Groenewegen E,Starks L,Forbes TA,Tan KS,Vanslambrouck JM,Holloway EM,Chen YH,Jain S,Spence JR,Little MH

Thu Apr 01 00:00:00 EDT 2021

Renal Natriuretic Peptide Receptor-C Deficiency Attenuates NaCl Cotransporter Activity in Angiotensin II-Induced Hypertension.

Hypertension (Dallas, Tex. : 1979)

Shao S,Li XD,Lu YY,Li SJ,Chen XH,Zhou HD,He S,Guo YT,Lu X,Gao PJ,Wang JG

Wed Mar 03 00:00:00 EST 2021

Metformin and Inhibition of Transforming Growth Factor-Beta Stimulate In Vitro Transport in Primary Renal Tubule Cells.

Tissue engineering. Part A

Love H,Evans R,Humes HD,Roy S,Zent R,Harris R,Wilson M,Fissell WH

Thu Oct 01 00:00:00 EDT 2020

Metformin and Inhibition of Transforming Growth Factor-Beta Stimulate In Vitro Transport in Primary Renal Tubule Cells.

Tissue engineering. Part A

Love H,Evans R,Humes HD,Roy S,Zent R,Harris R,Wilson M,Fissell WH

Thu Oct 01 00:00:00 EDT 2020

Hnf4a Is Required for the Development of Cdh6-Expressing Progenitors into Proximal Tubules in the Mouse Kidney.

Journal of the American Society of Nephrology : JASN

Marable SS,Chung E,Park JS

Sun Nov 01 00:00:00 EDT 2020

Identification and characterization of two morphologically distinct stem cell subpopulations from human urine samples.

Science China. Life sciences

Chen AJ,Pi JK,Hu JG,Huang YZ,Gao HW,Li SF,Li-Ling J,Xie HQ

Fri May 01 00:00:00 EDT 2020

Geniposide in Gardenia jasminoides var. radicans Makino modulates blood pressure via inhibiting WNK pathway mediated by the estrogen receptors.

The Journal of pharmacy and pharmacology

Fu Y,Yuan PP,Cao YG,Ke YY,Zhang Q,Hou Y,Zhang YL,Feng WS,Zheng XK

Tue Dec 01 00:00:00 EST 2020

Na⁺-K⁺-2Cl^- cotransporter 2 located in the human and murine gastric mucosa is involved in secretagogue-induced gastric acid secretion and is downregulated in lipopolysaccharide-treated mice.

European journal of pharmacology

Zheng LF,Ji T,Guo ZH,Wang T,Xiu XL,Liu XY,Li SC,Sun L,Xue H,Zhang Y,Zhu JX

Wed Aug 05 00:00:00 EDT 2020

Na⁺-K⁺-2Cl^- cotransporter 2 located in the human and murine gastric mucosa is involved in secretagogue-induced gastric acid secretion and is downregulated in lipopolysaccharide-treated mice.

European journal of pharmacology

Zheng LF,Ji T,Guo ZH,Wang T,Xiu XL,Liu XY,Li SC,Sun L,Xue H,Zhang Y,Zhu JX

Wed Aug 05 00:00:00 EDT 2020

Na⁺-K⁺-2Cl^- cotransporter 2 located in the human and murine gastric mucosa is involved in secretagogue-induced gastric acid secretion and is downregulated in lipopolysaccharide-treated mice.

European journal of pharmacology

Zheng LF,Ji T,Guo ZH,Wang T,Xiu XL,Liu XY,Li SC,Sun L,Xue H,Zhang Y,Zhu JX

Wed Aug 05 00:00:00 EDT 2020

Na⁺-K⁺-2Cl^- cotransporter 2 located in the human and murine gastric mucosa is involved in secretagogue-induced gastric acid secretion and is downregulated in lipopolysaccharide-treated mice.

European journal of pharmacology

Zheng LF,Ji T,Guo ZH,Wang T,Xiu XL,Liu XY,Li SC,Sun L,Xue H,Zhang Y,Zhu JX

Wed Aug 05 00:00:00 EDT 2020

β-catenin regulates the formation of multiple nephron segments in the mouse kidney.

Scientific reports

Deacon P,Concodora CW,Chung E,Park JS

Mon Nov 04 00:00:00 EST 2019

A Toolbox to Characterize Human Induced Pluripotent Stem Cell-Derived Kidney Cell Types and Organoids.

Journal of the American Society of Nephrology : JASN

Vanslambrouck JM,Wilson SB,Tan KS,Soo JY,Scurr M,Spijker HS,Starks LT,Neilson A,Cui X,Jain S,Little MH,Howden SE

Tue Oct 01 00:00:00 EDT 2019

EED, a member of the polycomb group, is required for nephron differentiation and the maintenance of nephron progenitor cells.

Development (Cambridge, England)

Zhang L,Ettou S,Khalid M,Taglienti M,Jain D,Jung YL,Seager C,Liu Y,Ng KH,Park PJ,Kreidberg JA

Wed Jul 18 00:00:00 EDT 2018

EED, a member of the polycomb group, is required for nephron differentiation and the maintenance of nephron progenitor cells.

Development (Cambridge, England)

Zhang L,Ettou S,Khalid M,Taglienti M,Jain D,Jung YL,Seager C,Liu Y,Ng KH,Park PJ,Kreidberg JA

Wed Jul 18 00:00:00 EDT 2018

Reduced Abd-B Hox function during kidney development results in lineage infidelity.

Developmental biology

Magella B,Mahoney R,Adam M,Potter SS

Fri Jun 15 00:00:00 EDT 2018

Reduced Abd-B Hox function during kidney development results in lineage infidelity.

Developmental biology

Magella B,Mahoney R,Adam M,Potter SS

Fri Jun 15 00:00:00 EDT 2018

Hnf4a deletion in the mouse kidney phenocopies Fanconi renotubular syndrome.

JCI insight

Marable SS,Chung E,Adam M,Potter SS,Park JS

Thu Jul 26 00:00:00 EDT 2018

Hnf4a deletion in the mouse kidney phenocopies Fanconi renotubular syndrome.

JCI insight

Marable SS,Chung E,Adam M,Potter SS,Park JS

Thu Jul 26 00:00:00 EDT 2018

A Sall1-NuRD interaction regulates multipotent nephron progenitors and is required for loop of Henle formation.

Development (Cambridge, England)

Basta JM,Robbins L,Denner DR,Kolar GR,Rauchman M

Fri Sep 01 00:00:00 EDT 2017

A Sall1-NuRD interaction regulates multipotent nephron progenitors and is required for loop of Henle formation.

Development (Cambridge, England)

Basta JM,Robbins L,Denner DR,Kolar GR,Rauchman M

Fri Sep 01 00:00:00 EDT 2017

Notch signaling promotes nephrogenesis by downregulating Six2.

Development (Cambridge, England)

Chung E,Deacon P,Marable S,Shin J,Park JS

Tue Nov 01 00:00:00 EDT 2016

Notch signaling promotes nephrogenesis by downregulating Six2.

Development (Cambridge, England)

Chung E,Deacon P,Marable S,Shin J,Park JS

Tue Nov 01 00:00:00 EDT 2016

Bioinformatics

Protein Aliases: BSC1; bumetanide-sensitive cotransporter type 1; Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 1; bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2; Kidney-specific Na-K-Cl symporter; MGC48843; mNKCC2; Na-K-2Cl cotransporter; Na-K-2Cl cotransporter 2; NKCC2; NKCC2A variant A; solute carrier family 12 (sodium/potassium/chloride transporter), member 1; solute carrier family 12 (sodium/potassium/chloride transporters), member 1; Solute carrier family 12 member 1; Solute carrier family 12 member 1 (bumetanide-sensitive sodium-[potassium]-chloride cotransporter); Solute carrier family 12, member 1 (bumetanide-sensitive sodium-[potassium]-chloride cotransporter)

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Gene Aliases: AI788571; BSC1; D630042G03Rik; mBSC1; NKCC2; SLC12A1; urehr3

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UniProt ID: (Human) Q13621, (Mouse) P55014

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Entrez Gene ID: (Human) 6557, (Mouse) 20495, (Rat) 25065

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Function(s)

sodium:potassium:chloride symporter activity transporter activity protein binding symporter activity cation:chloride symporter activity rubidium ion transmembrane transporter activity secondary carrier transporter

Process(es)

transport ion transport potassium ion transport sodium ion transport diterpenoid metabolic process protein insertion into membrane from inner side ion transmembrane transport chloride transmembrane transport kidney development chloride transport excretion renal rubidium ion absorption chemical homeostasis transmembrane transport positive regulation of calcineurin-NFAT signaling cascade cellular hyperosmotic salinity response cation transmembrane transport renal sodium ion absorption

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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NKCC2 Polyclonal Antibody

NKCC2 Antibody (18970-1-AP) in IHC (P)

Product Details

18970-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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