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NKp30 Antibody (PA5-104376) in ICC/IF
Immunocytochemistry analysis of NKp30 in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with NKp30 polyclonal antibody (Product # PA5-104376) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-104376
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human NCR3(Accession O14931), corresponding to amino acid residues A103-S153.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2853684
Product Specific Information
Antibody detects endogenous levels of total NCR3.
Target Information
The protein encoded by this gene is a natural cytotoxicity receptor (NCR) that may aid NK cells in the lysis of tumor cells. The encoded protein interacts with CD3-zeta (CD247), a T-cell receptor. A single nucleotide polymorphism in the 5' untranslated region of this gene has been associated with mild malaria suceptibility. Three transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, May 2010].
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Activating natural killer receptor p30; activating NK-A1 receptor; CD337; lymphocyte antigen 117; Natural cytotoxicity triggering receptor 3; Natural killer cell p30-related protein; natural killer cell receptor; NK receptor 1c7; NK-p30
Gene Aliases: 1C7; CD337; LY117; MALS; NCR3; NKp30
UniProt ID: (Human) O14931
Entrez Gene ID: (Human) 259197, (Rat) 294251
positive regulation of natural killer cell mediated cytotoxicity
positive regulation of natural killer cell mediated cytotoxicity directed against tumor cell target
inflammatory response
immune response
homophilic cell adhesion via plasma membrane adhesion molecules
heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules
cell recognition
susceptibility to natural killer cell mediated cytotoxicity
regulation of immune response
susceptibility to T cell mediated cytotoxicity
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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