Invitrogen

NLRP3 Polyclonal Antibody

13 Published Figures

21 References

View all (29) NLRP3 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (9)
Published Figures (13)

NLRP3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 22

NLRP3 Antibody (PA5-79740) in IHC (P)

Immunohistochemical analysis of CIAS1/NALP3 in paraffin-embedded section of rat spleen tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL rabbit anti-CIAS1/NALP3 antibody (Product # PA5-79740) overnight at 4°C. Cy3 Conjugated Goat Anti-Ra... View More

NLRP3 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

NLRP3 Antibody in Immunocytochemistry (ICC/IF)

NLRP3 Antibody in Immunohistochemistry (IHC)

NLRP3 Antibody in Western Blot, Immunohistochemistry (WB, IHC)

NLRP3 Antibody in Western Blot, Immunoprecipitation (WB, IP)

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Product Details

PA5-79740

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-0.5 µg/mL

View 6 publications 6 publications

Immunohistochemistry (IHC)

View 8 publications 8 publications

Immunohistochemistry (Paraffin) (IHC (P))

2-5 µg/mL

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

View 4 publications 4 publications

Flow Cytometry (Flow)

1-3 µg/1x10^6 cells

Immunoprecipitation (IP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human CIAS1 (12-31aa RYLEDLEDVDLKKFKMHLED).

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

500 µg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 4mg trehalose

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2746855

Product Specific Information

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Target Information

This gene encodes a pyrin-like protein containing a pyrin domain, a nucleotide-binding site domain, and a leucine-rich repeat motif. This protein interacts with the apoptosis-associated speck-like protein PYCARD/ASC, which contains a caspase recruitment domain, and is a member of the NALP3 inflammasome complex. This complex functions as an upstream activator of NF-kappaB signaling, and it plays a role in the regulation of inflammation, the immune response, and apoptosis. Mutations in this gene are associated with familial cold autoinflammatory syndrome, and neonatal-onset multisystem inflammatory disease. Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. Alternative 5' UTR structures are suggested by available data; however, insufficient evidence is available to determine if all of the represented 5' UTR splice patterns are biologically valid.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Nlrp3 Deficiency Alleviates Lipopolysaccharide-Induced Acute Kidney Injury via Suppressing Renal Inflammation and Ferroptosis in Mice.

Biology

Li Z,Wang X,Peng Y,Yin H,Yu S,Zhang W,Ni X

Published figure using NLRP3 polyclonal antibody (Product # PA5-79740) in Immunohistochemistry (Paraffin)

Thu Aug 31 00:00:00 EDT 2023

AR-A014418, a glycogen synthase kinase-3β inhibitor, mitigates lipopolysaccharide-induced inflammation in rat dental pulp stem cells via NLR family pyrin domain containing 3 inflammasome impairment.

Journal of dental sciences

Xie H,Lin Y,Fang F

PA5-79740 was used in Western Blot and Immunocytochemistry to suggest that AR-A014418 significantly mitigates LPS-induced inflammation of rDPSCs by blocking the activation of the NLRP3 inflammasome.

Sun Oct 01 00:00:00 EDT 2023

AR-A014418, a glycogen synthase kinase-3β inhibitor, mitigates lipopolysaccharide-induced inflammation in rat dental pulp stem cells via NLR family pyrin domain containing 3 inflammasome impairment.

Journal of dental sciences

Xie H,Lin Y,Fang F

PA5-79740 was used in Western Blot and Immunocytochemistry to suggest that AR-A014418 significantly mitigates LPS-induced inflammation of rDPSCs by blocking the activation of the NLRP3 inflammasome.

Sun Oct 01 00:00:00 EDT 2023

Suppression of Macrophage Activation by Sodium Danshensu via HIF-1α/STAT3/NLRP3 Pathway Ameliorated Collagen-Induced Arthritis in Mice.

Molecules (Basel, Switzerland)

Wu D,Xu J,Jiao W,Liu L,Yu J,Zhang M,Chen G

PA5-79740 was used in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to demonstrate that SDSS displays anti-arthritic activity in CIA mice and prevents proinflammatory cytokines secretion in macrophages by suppressing the HIF-1α/STAT3/NLRP3 pathway.

Mon Feb 06 00:00:00 EST 2023

Suppression of Macrophage Activation by Sodium Danshensu via HIF-1α/STAT3/NLRP3 Pathway Ameliorated Collagen-Induced Arthritis in Mice.

Molecules (Basel, Switzerland)

Wu D,Xu J,Jiao W,Liu L,Yu J,Zhang M,Chen G

Mon Feb 06 00:00:00 EST 2023

Development of Neovasculature in Axially Vascularized Calcium Phosphate Cement Scaffolds.

Journal of functional biomaterials

Ouhaddi Y,Charbonnier B,Porge J,Zhang YL,Garcia I,Gbureck U,Grover L,Gilardino M,Harvey E,Makhoul N,Barralet J

PA5-79740 was used in Immunohistochemistry to investigate if sprouting was a general response to surgical injury or placement of an inorganic construct around the vessel.

Tue Feb 14 00:00:00 EST 2023

Development of Neovasculature in Axially Vascularized Calcium Phosphate Cement Scaffolds.

Journal of functional biomaterials

Ouhaddi Y,Charbonnier B,Porge J,Zhang YL,Garcia I,Gbureck U,Grover L,Gilardino M,Harvey E,Makhoul N,Barralet J

PA5-79740 was used in Immunohistochemistry to investigate if sprouting was a general response to surgical injury or placement of an inorganic construct around the vessel.

Tue Feb 14 00:00:00 EST 2023

Regional variances depict a unique glial-specific inflammatory response following closed-head injury.

Frontiers in cellular neuroscience

White MR,VandeVord PJ

PA5-79740 was used in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to show that overall these findings are consistent with glial changes reported following concussions and may contribute to subsequent symptoms.

Tue Mar 14 00:00:00 EDT 2023

The protective effect of luteolin on the depression-related dry eye disorder through Sirt1/NF-κB/NLRP3 pathway.

Aging

Xie M,Wang H,Gao T,Peng J,Meng P,Zhang X,Guo D,Liu G,Shi J,Peng Q

PA5-79740 was used in Immunohistochemistry to investigate the therapeutic effect of luteolin on the depression-related dry eye disorder.

Wed Jan 11 00:00:00 EST 2023

Oridonin relieves depressive-like behaviors by inhibiting neuroinflammation and autophagy impairment in rats subjected to chronic unpredictable mild stress.

Phytotherapy research : PTR

Liang L,Wang H,Hu Y,Bian H,Xiao L,Wang G

Published figure using NLRP3 polyclonal antibody (Product # PA5-79740) in Western Blot

Mon Aug 01 00:00:00 EDT 2022

Oridonin relieves depressive-like behaviors by inhibiting neuroinflammation and autophagy impairment in rats subjected to chronic unpredictable mild stress.

Phytotherapy research : PTR

Liang L,Wang H,Hu Y,Bian H,Xiao L,Wang G

Published figure using NLRP3 polyclonal antibody (Product # PA5-79740) in Western Blot

Mon Aug 01 00:00:00 EDT 2022

Anti-Inflammatory Properties of KLS-13019: a Novel GPR55 Antagonist for Dorsal Root Ganglion and Hippocampal Cultures.

Journal of molecular neuroscience : MN

Brenneman DE,Kinney WA,McDonnell ME,Zhao P,Abood ME,Ward SJ

PA5-79740 was used in Immunohistochemistry to test for reversibility of paclitaxel-induced increases in GPR55.

Thu Sep 01 00:00:00 EDT 2022

Stem Cells From Human Exfoliated Deciduous Teeth Alleviate Liver Cirrhosis via Inhibition of Gasdermin D-Executed Hepatocyte Pyroptosis.

Frontiers in immunology

Chen P,Zhou YK,Han CS,Chen LJ,Wang YM,Zhuang ZM,Lin S,Zhou YH,Jiang JH,Yang RL

PA5-79740 was used in Immunocytochemistry, Immunohistochemistry, and Western Blotting to assess the therapeutic impact of stem cells from human exfoliated deciduous teeth (SHED) on CCl4-induced liver cirrhosis by examining their ability to inhibit NLRP3 inflammasome activation and gasdermin D (GSDMD)-mediated pyroptosis in hepatocytes.

Thu Jun 02 00:00:00 EDT 2022

Stem Cells From Human Exfoliated Deciduous Teeth Alleviate Liver Cirrhosis via Inhibition of Gasdermin D-Executed Hepatocyte Pyroptosis.

Frontiers in immunology

Chen P,Zhou YK,Han CS,Chen LJ,Wang YM,Zhuang ZM,Lin S,Zhou YH,Jiang JH,Yang RL

Thu Jun 02 00:00:00 EDT 2022

Stem Cells From Human Exfoliated Deciduous Teeth Alleviate Liver Cirrhosis via Inhibition of Gasdermin D-Executed Hepatocyte Pyroptosis.

Frontiers in immunology

Chen P,Zhou YK,Han CS,Chen LJ,Wang YM,Zhuang ZM,Lin S,Zhou YH,Jiang JH,Yang RL

Thu Jun 02 00:00:00 EDT 2022

Stem Cells From Human Exfoliated Deciduous Teeth Alleviate Liver Cirrhosis via Inhibition of Gasdermin D-Executed Hepatocyte Pyroptosis.

Frontiers in immunology

Chen P,Zhou YK,Han CS,Chen LJ,Wang YM,Zhuang ZM,Lin S,Zhou YH,Jiang JH,Yang RL

Thu Jun 02 00:00:00 EDT 2022

Electroacupuncture Inhibits NLRP3 Activation by Regulating CMPK2 After Spinal Cord Injury.

Frontiers in immunology

Chen Y,Wu L,Shi M,Zeng D,Hu R,Wu X,Han S,He K,Xu H,Shao X,Ma R

Published figure using NLRP3 polyclonal antibody (Product # PA5-79740) in Immunohistochemistry

Tue Apr 12 00:00:00 EDT 2022

Electroacupuncture Inhibits NLRP3 Activation by Regulating CMPK2 After Spinal Cord Injury.

Frontiers in immunology

Chen Y,Wu L,Shi M,Zeng D,Hu R,Wu X,Han S,He K,Xu H,Shao X,Ma R

Published figure using NLRP3 polyclonal antibody (Product # PA5-79740) in Immunohistochemistry

Tue Apr 12 00:00:00 EDT 2022

Electroacupuncture Ameliorates Mechanical Allodynia of a Rat Model of CRPS-I via Suppressing NLRP3 Inflammasome Activation in Spinal Cord Dorsal Horn Neurons.

Frontiers in cellular neuroscience

Zhang Y,Chen R,Hu Q,Wang J,Nie H,Yin C,Li Y,Wei H,Liu B,Tai Y,Fang J,Shao X,Jin X,Fang J,Liu B

PA5-79740 was used in Immunohistochemistry to demonstrate that spinal NLRP3 inflammasome activation contributes to mechanical allodynia of the rat model of CRPS-I and EA ameliorates mechanical allodynia through inhibiting NLRP3 inflammasome activation in SCDH neurons.

Sat Jul 16 00:00:00 EDT 2022

Erythropoietin Abrogates Post-Ischemic Activation of the NLRP3, NLRC4, and AIM2 Inflammasomes in Microglia/Macrophages in a TAK1-Dependent Manner.

Translational stroke research

Heinisch O,Zeyen T,Goldmann T,Prinz M,Huber M,Jung J,Arik E,Habib S,Slowik A,Reich A,Schulz JB,Habib P

PA5-79740 was used in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to provide evidence that Mi/MΦ TAK1 regulates the expression and activation of the NLRP3, NLRC4, AIM2 inflammasomes.

Wed Jun 01 00:00:00 EDT 2022

Erythropoietin Enhances Post-ischemic Migration and Phagocytosis and Alleviates the Activation of Inflammasomes in Human Microglial Cells.

Frontiers in cellular neuroscience

Arik E,Heinisch O,Bienert M,Gubeljak L,Slowik A,Reich A,Schulz JB,Wilhelm T,Huber M,Habib P

PA5-79740 was used in Immunocytochemistry-immunoflourescence to provide evidence that EPO-conveyed anti-inflammatory actions might be mediated via the regulation of the inflammasomes.

Sat Jul 16 00:00:00 EDT 2022

NU9056, a KAT 5 Inhibitor, Treatment Alleviates Brain Dysfunction by Inhibiting NLRP3 Inflammasome Activation, Affecting Gut Microbiota, and Derived Metabolites in LPS-Treated Mice.

Frontiers in nutrition

Chen L,Qing W,Yi Z,Lin G,Peng Q,Zhou F

PA5-79740 was used in Immunohistochemistry (Paraffin) to investigate if NU9056 might effectively alleviate lipopolysaccharide-induced cognitive impairment and emotional disorder in experimental mice by inhibiting the NLRP3 inflammasome.

Sat Jul 31 00:00:00 EDT 2021

NU9056, a KAT 5 Inhibitor, Treatment Alleviates Brain Dysfunction by Inhibiting NLRP3 Inflammasome Activation, Affecting Gut Microbiota, and Derived Metabolites in LPS-Treated Mice.

Frontiers in nutrition

Chen L,Qing W,Yi Z,Lin G,Peng Q,Zhou F

Sat Jul 31 00:00:00 EDT 2021

NLRP3 Inflammasome: A Potential Target in Isoflurane Pretreatment Alleviates Stroke-Induced Retinal Injury in Diabetes.

Frontiers in cellular neuroscience

Lin HB,Lin YH,Zhang JY,Guo WJ,Ovcjak A,You ZJ,Feng ZP,Sun HS,Li FX,Zhang HF

PA5-79740 was used in Immunohistochemistry to investigate whether isoflurane offers retinal protection against stroke-induced retinal injury in diabetes.

Tue Jul 27 00:00:00 EDT 2021

NLRP3 Inflammasome: A Potential Target in Isoflurane Pretreatment Alleviates Stroke-Induced Retinal Injury in Diabetes.

Frontiers in cellular neuroscience

Lin HB,Lin YH,Zhang JY,Guo WJ,Ovcjak A,You ZJ,Feng ZP,Sun HS,Li FX,Zhang HF

PA5-79740 was used in Immunohistochemistry to investigate whether isoflurane offers retinal protection against stroke-induced retinal injury in diabetes.

Tue Jul 27 00:00:00 EDT 2021

Bioinformatics

Protein Aliases: Angiotensin/vasopressin receptor AII/AVP-like; Caterpiller protein 1.1; CLR1.1; cold autoinflammatory syndrome 1 homolog; cold autoinflammatory syndrome 1 protein; Cold-induced autoinflammatory syndrome 1 protein; Cryopyrin; FLJ95925; NACHT domain-, leucine-rich repeat-, and PYD-containing protein 3; NACHT, LRR and PYD containing protein 3; NACHT, LRR and PYD domains-containing protein 3; NLR family, pyrin domain containing 3; nucleotide-binding oligomerization domain, leucine rich repeat and pyrin domain containing 3; PYRIN-containing APAF1-like protein 1

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Gene Aliases: AGTAVPRL; AII; AVP; C1orf7; CIAS1; CLR1.1; FCAS; FCAS1; FCU; MWS; NALP3; NLRP3; PYPAF1

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UniProt ID: (Human) Q5JQS9, (Rat) D4A523

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Entrez Gene ID: (Human) 114548, (Rat) 287362

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Function(s)

protein binding ATP binding transcription factor binding peptidoglycan binding sequence-specific DNA binding molecular_function non-motor microtubule binding protein

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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NLRP3 Polyclonal Antibody

NLRP3 Antibody (PA5-79740) in IHC (P)

Product Details

PA5-79740

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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