Invitrogen

NLRP3 Monoclonal Antibody (768319)

11 Published Figures

13 References

View all (29) NLRP3 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (5)
Published Figures (11)

NLRP3 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 16

NLRP3 Antibody (MA5-23919) in ICC/IF

Immunocytochemistry analysis of NLRP3 in immersion fixed RAW 264.7 mouse monocyte/macrophage cell line. Samples were incubated in NLRP3 monoclonal antibody (Product # MA5-23919) using a dilution of 10 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.

NLRP3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

Product Details

MA5-23919

Applications

Tested Dilution

Publications

Western Blot (WB)

2 µg/mL

View 6 publications 6 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

5-25 µg/mL

Immunohistochemistry (PFA fixed) (IHC (PFA))

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

8-25 µg/mL

View 2 publications 2 publications

Flow Cytometry (Flow)

2.5 µg per million cells

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Fungi, Human, Mouse

Host/Isotype

Rat / IgG2a

Class

Monoclonal

Type

Antibody

Clone

768319

Immunogen

E. coli-derived recombinant mouse NLRP3/NALP3 Met1-Arg153

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.5 mg/mL

Purification

Protein A/G

Storage buffer

PBS with 5% trehalose

Contains

No Preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2605972

Product Specific Information

In direct ELISAs, 100% cross-reactivity with recombinant human NLRP3/NALP3 is observed.

Reconstitute in sterile PBS to a final concentration of 0.5 mg/mL.

Target Information

This gene encodes a pyrin-like protein containing a pyrin domain, a nucleotide-binding site domain, and a leucine-rich repeat motif. This protein interacts with the apoptosis-associated speck-like protein PYCARD/ASC, which contains a caspase recruitment domain, and is a member of the NALP3 inflammasome complex. This complex functions as an upstream activator of NF-kappaB signaling, and it plays a role in the regulation of inflammation, the immune response, and apoptosis. Mutations in this gene are associated with familial cold autoinflammatory syndrome, and neonatal-onset multisystem inflammatory disease. Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. Alternative 5' UTR structures are suggested by available data; however, insufficient evidence is available to determine if all of the represented 5' UTR splice patterns are biologically valid.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

CircSFMBT2-OA alleviates chondrocyte apoptosis and extracellular matrix degradation through repressing NF-κB/NLRP3 inflammasome activation.

Heliyon

He A,Liu Y,Zhang R,Mao Y,Liu W

Published figure using NLRP3 monoclonal antibody (Product # MA5-23919) in Western Blot

Thu Jun 01 00:00:00 EDT 2023

Icariin restrains NLRP3 inflammasome-mediated Th2 immune responses and ameliorates atopic dermatitis through modulating a novel lncRNA MALAT1/miR-124-3p axis.

Pharmaceutical biology

Zhao W,Yu HH,Meng WW,Liu AM,Zhang BX,Wang Y,Li J,Wang L,Fang YF

Published figure using NLRP3 monoclonal antibody (Product # MA5-23919) in Western Blot

Fri Dec 01 00:00:00 EST 2023

miR-25-3p ameliorates SAE by targeting the TLR4/NLRP3 axis.

Metabolic brain disease

Luo XY,Ying JH,Wang QS

MA5-23919 was used in Immunohistochemistry-immunofluorescence to suggest that miR-25-3p may be a potential target for SAE diagnosis and treatment.

Mon Aug 01 00:00:00 EDT 2022

Baicalin Alleviates Contrast-Induced Acute Kidney Injury Through ROS/NLRP3/Caspase-1/GSDMD Pathway-Mediated Proptosis in vitro.

Drug design, development and therapy

Li Y,Wang J,Huang D,Yu C

Published figure using NLRP3 monoclonal antibody (Product # MA5-23919) in Western Blot

Thu Oct 06 00:00:00 EDT 2022

Modified Citrus Pectin Alleviates Cerebral Ischemia/Reperfusion Injury by Inhibiting NLRP3 Inflammasome Activation via TLR4/NF-ĸB Signaling Pathway in Microglia.

Journal of inflammation research

Cui Y,Zhang NN,Wang D,Meng WH,Chen HS

MA5-23919 was used in Immunohistochemistry-immunofluorescence to report that MCP exerts neuroprotective effects in ischemic stroke through blocking galectin-3, which may be mediated by inhibiting the activation of NLRP3 inflammasome via TLR4/NF-κB signaling pathway in microglia.

Sat Jul 16 00:00:00 EDT 2022

Huaier extract suppresses non-small cell lung cancer progression through activating NLRP3-dependent pyroptosis.

Anatomical record (Hoboken, N.J. : 2007)

Xie J,Zhuan B,Wang H,Wang Y,Wang X,Yuan Q,Yang Z

Published figure using NLRP3 monoclonal antibody (Product # MA5-23919) in Western Blot

Mon Feb 01 00:00:00 EST 2021

Extracellular ASC exacerbated the recurrent ischemic stroke in an NLRP3-dependent manner.

Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism

He XF,Zeng YX,Li G,Feng YK,Wu C,Liang FY,Zhang Y,Lan Y,Xu GQ,Pei Z

MA5-23919 was used in Immunocytochemistry to show that a single stroke onset increases the nuclear factor-κB (NF-κB), NLR family CARD domain containing protein 4 (NLRC4), and absent in melanoma 2 (AIM2) inflammasomes, as well as the mRNA levels of NLRP3, using a photothrombotic mouse model of single stroke.

Fri May 01 00:00:00 EDT 2020

Sauchinone Protects Renal Mesangial Cell Dysfunction against Angiotensin II by Improving Renal Fibrosis and Inflammation.

International journal of molecular sciences

Yoon JJ,Lee HK,Kim HY,Han BH,Lee HS,Lee YJ,Kang DG

MA5-23919 was used in Immunocytochemistry to investigate the role of sauchinone in mesangial cell proliferation and fibrosis induced by angiotensin II, as well as the underlying mechanisms of these processes.

Wed Sep 23 00:00:00 EDT 2020

Sauchinone Protects Renal Mesangial Cell Dysfunction against Angiotensin II by Improving Renal Fibrosis and Inflammation.

International journal of molecular sciences

Yoon JJ,Lee HK,Kim HY,Han BH,Lee HS,Lee YJ,Kang DG

Wed Sep 23 00:00:00 EDT 2020

Microbial BMAA elicits mitochondrial dysfunction, innate immunity activation, and Alzheimer's disease features in cortical neurons.

Journal of neuroinflammation

Silva DF,Candeias E,Esteves AR,Magalhães JD,Ferreira IL,Nunes-Costa D,Rego AC,Empadinhas N,Cardoso SM

MA5-23919 was used in Western Blotting to show that neurons, alone, can mount inflammatory responses, a role previously attributed exclusively to glial cells.

Thu Nov 05 00:00:00 EST 2020

Microbial BMAA elicits mitochondrial dysfunction, innate immunity activation, and Alzheimer's disease features in cortical neurons.

Journal of neuroinflammation

Silva DF,Candeias E,Esteves AR,Magalhães JD,Ferreira IL,Nunes-Costa D,Rego AC,Empadinhas N,Cardoso SM

MA5-23919 was used in Western Blotting to show that neurons, alone, can mount inflammatory responses, a role previously attributed exclusively to glial cells.

Thu Nov 05 00:00:00 EST 2020

Paeoniflorin relieves LPS-induced inflammatory pain in mice by inhibiting NLRP3 inflammasome activation via transient receptor potential vanilloid 1.

Journal of leukocyte biology

Yin N,Gao Q,Tao W,Chen J,Bi J,Ding F,Wang Z

MA5-23919 was used in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to suggest that PF can be as a potential pharmacological agent for inflammatory pain and thus deserves more attention and further investigation.

Wed Jul 01 00:00:00 EDT 2020

Monitoring Inflammasome Priming and Activation in Response to Candida albicans.

Current protocols in microbiology

Santana DJ,Anderson FM,O'Meara TR

MA5-23919 was used in Miscellaneous to describe methods for investigating the interactions between C. albicans and host macrophages, with a focus on the inflammasome.

Tue Dec 01 00:00:00 EST 2020

Hyperactivity of Innate Immunity Triggers Pain via TLR2-IL-33-Mediated Neuroimmune Crosstalk.

Cell reports

Huang J,Gandini MA,Chen L,M'Dahoma S,Stemkowski PL,Chung H,Muruve DA,Zamponi GW

MA5-23919 was used in Immunohistochemistry (PFA fixed) to report that hyperactivity of innate immunity primes and initiates pain states via the TLR2-interleukin-33 (IL-33) axis.

Tue Oct 06 00:00:00 EDT 2020

Bioinformatics

Protein Aliases: Angiotensin/vasopressin receptor AII/AVP-like; Caterpiller protein 1.1; CLR1.1; cold autoinflammatory syndrome 1 homolog; cold autoinflammatory syndrome 1 protein; Cold autoinflammatory syndrome 1 protein homolog; Cold-induced autoinflammatory syndrome 1 protein; Cryopyrin; FLJ95925; mast cell maturation inducible protein 1; Mast cell maturation-associated-inducible protein 1; NACHT domain-, leucine-rich repeat-, and PYD-containing protein 3; NACHT, LRR and PYD containing protein 3; NACHT, LRR and PYD domains-containing protein 3; NACHT/LRR/pyrin domain-containing protein 3; NLR family, pyrin domain containing 3; nucleotide-binding oligomerization domain, leucine rich repeat and pyrin domain containing 3; PYRIN-containing APAF1-like protein 1

View more View less

Gene Aliases: AGTAVPRL; AII; AII/AVP; AVP; C1orf7; CIAS1; CLR1.1; FCAS; FCAS1; FCU; Mmig1; MWS; NALP3; NLRP3; PYPAF1

View more View less

UniProt ID: (Human) Q5JQS9, (Mouse) Q8R4B8

View more View less

Entrez Gene ID: (Human) 114548, (Mouse) 216799

View more View less

Function(s)

protein binding ATP binding transcription factor binding peptidoglycan binding sequence-specific DNA binding nucleotide binding non-motor microtubule binding protein

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

NLRP3 Monoclonal Antibody (768319)

NLRP3 Antibody (MA5-23919) in ICC/IF

Product Details

MA5-23919

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!