Invitrogen

NMDAR2A Polyclonal Antibody

NMDAR2A Antibody in Immunohistochemistry (Frozen) (IHC (F))

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NMDAR2A Antibody (A-6473) in IHC (F)

Immunohistochemistry using NMDA Receptor Subunit 2A Polyclonal Antibody, Rabbit: Rat brain cryosections labeled with anti-NMDA receptor, subunit 2A (rat), rabbit IgG fraction (Product # A-6473) and detected using Alexa Fluor® 488 goat anti-rabbit IgG antibody (Product # A-11008). The tissue was also labeled with Alexa Fluor® 594 anti-glial fibrillary acidic protein antibody (Product # A-21295) and counterst... View More

NMDAR2A Antibody in Immunocytochemistry (ICC/IF)

NMDAR2A Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)

NMDAR2A Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

NMDAR2A Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

A-6473

Applications

Tested Dilution

Publications

Western Blot (WB)

1:100-1:500

View 17 publications 17 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Frozen) (IHC (F))

1:500-1:5,000

Immunocytochemistry (ICC/IF)

View 3 publications 3 publications

ELISA (ELISA)

Assay-dependent

Immunoprecipitation (IP)

3 µL

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Fusion protein containing amino acid residues 1253-1391 of the C-terminal region of rat brain subunit 2A

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

IgG fraction

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2536209

Product Specific Information

This antibody recognizes the 180 kDa 2A subunit from rat, mouse and human, and does not cross-react with the 2B or 2C subunits.

Storage and reconstitution: when stored at -20ºC or below, the lyophilized antibody should retain full activity for over one year. Reconstitute using 50 µL PBS, pH 7.4, to yield a 0.2 mg/mL solution. If storing at 0-4ºC, add sodium azide to a final concentration of 2mM. For longer storage, divide the solution into aliquots and freeze at -20ºC, avoiding freeze/thaw cycles.

Target Information

N-methyl-D-aspartate (NMDA) receptors are a class of ionotropic glutamate-gated ion channels. These receptors have been shown to be involved in long-term potentiation, an activity-dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning. NMDA receptor channels are heteromers composed of the key receptor subunit NMDAR1 (GRIN1) and 1 or more of the 4 NMDAR2 subunits: NMDAR2A (GRIN2A), NMDAR2B (GRIN2B), NMDAR2C (GRIN2C) and NMDAR2D (GRIN2D). Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Loss of spines in the prelimbic cortex is detrimental to working memory in mice with early-life adversity.

Molecular psychiatry

Xu L,Liu Y,Long J,He X,Xie F,Yin Q,Chen M,Long D,Chen Y

A-6473 was used in Western Blotting to investigate the impact of early-life adversity (ELA) on dendritic spines, postsynaptic puncta, and glutamatergic transmission in the prelimbic cortex, finding that ELA results in the loss of spines, postsynaptic puncta, and depressed glutamatergic transmission, which contributes to poor spatial working memory, and exciting Thy1-expressing PrL pyramidal cells can improve working memory in ELA mice.

Tue Aug 01 00:00:00 EDT 2023

Alteration of Ethanol Reward by Prior Mephedrone Exposure: The Role of Age and Matrix Metalloproteinase-9 (MMP-9).

International journal of molecular sciences

Grochecki P,Smaga I,Marszalek-Grabska M,Lopatynska-Mazurek M,Slowik T,Gibula-Tarlowska E,Kedzierska E,Listos J,Filip M,Kotlinska JH

A-6473 was used in ELISA, Immunohistochemistry (Frozen), Immunoprecipitation, Western Blot to suggest that prior mephedrone exposure differentially alters ethanol reward in adolescent and adult rats.

Mon Feb 14 00:00:00 EST 2022

The NMDA Receptor Subunit (GluN1 and GluN2A) Modulation Following Different Conditions of Cocaine Abstinence in Rat Brain Structures.

Neurotoxicity research

Smaga I,Wydra K,Suder A,Frankowska M,Sanak M,Caffino L,Fumagalli F,Filip M

Published figure using NMDAR2A polyclonal antibody (Product # A-6473) in Western Blot

Tue Jun 01 00:00:00 EDT 2021

The NMDA Receptor Subunit (GluN1 and GluN2A) Modulation Following Different Conditions of Cocaine Abstinence in Rat Brain Structures.

Neurotoxicity research

Smaga I,Wydra K,Suder A,Frankowska M,Sanak M,Caffino L,Fumagalli F,Filip M

Published figure using NMDAR2A polyclonal antibody (Product # A-6473) in Western Blot

Tue Jun 01 00:00:00 EDT 2021

Adulthood systemic inflammation accelerates the trajectory of age-related cognitive decline.

Aging

Barter J,Kumar A,Bean L,Ciesla M,Foster TC

A-6473 was used in Western Blotting to suggest that the history of systemic inflammation is one component of environmental factors that contribute to the resilience or susceptibility to age-related brain changes and associated trajectory of cognitive decline.

Wed Sep 29 00:00:00 EDT 2021

Adulthood systemic inflammation accelerates the trajectory of age-related cognitive decline.

Aging

Barter J,Kumar A,Bean L,Ciesla M,Foster TC

Wed Sep 29 00:00:00 EDT 2021

Effects of Mephedrone and Amphetamine Exposure during Adolescence on Spatial Memory in Adulthood: Behavioral and Neurochemical Analysis.

International journal of molecular sciences

Grochecki P,Smaga I,Lopatynska-Mazurek M,Gibula-Tarlowska E,Kedzierska E,Listos J,Talarek S,Marszalek-Grabska M,Hubalewska-Mazgaj M,Korga-Plewko A,Dudka J,Marzec Z,Filip M,Kotlinska JH

Published figure using NMDAR2A polyclonal antibody (Product # A-6473) in Western Blot

Fri Jan 08 00:00:00 EST 2021

Effects of Mephedrone and Amphetamine Exposure during Adolescence on Spatial Memory in Adulthood: Behavioral and Neurochemical Analysis.

International journal of molecular sciences

Grochecki P,Smaga I,Lopatynska-Mazurek M,Gibula-Tarlowska E,Kedzierska E,Listos J,Talarek S,Marszalek-Grabska M,Hubalewska-Mazgaj M,Korga-Plewko A,Dudka J,Marzec Z,Filip M,Kotlinska JH

Published figure using NMDAR2A polyclonal antibody (Product # A-6473) in Western Blot

Fri Jan 08 00:00:00 EST 2021

An activity-dependent local transport regulation via degradation and synthesis of KIF17 underlying cognitive flexibility.

Science advances

Iwata S,Morikawa M,Takei Y,Hirokawa N

A-6473 was used in Western Blot, Immunocytochemistry to show that the molecular motor KIF17 is locally degraded and synthesized in an N-methyl-d-aspartate receptor (NMDAR)-mediated activity-dependent manner.

Tue Dec 01 00:00:00 EST 2020

An activity-dependent local transport regulation via degradation and synthesis of KIF17 underlying cognitive flexibility.

Science advances

Iwata S,Morikawa M,Takei Y,Hirokawa N

Tue Dec 01 00:00:00 EST 2020

An activity-dependent local transport regulation via degradation and synthesis of KIF17 underlying cognitive flexibility.

Science advances

Iwata S,Morikawa M,Takei Y,Hirokawa N

Tue Dec 01 00:00:00 EST 2020

Cocaine Self-Administration and Abstinence Modulate NMDA Receptor Subunits and Active Zone Proteins in the Rat Nucleus Accumbens.

Molecules (Basel, Switzerland)

Smaga I,Wydra K,Frankowska M,Fumagalli F,Sanak M,Filip M

A-6473 was used in Western Blotting to investigate the effect of cocaine self-administration and different conditions of cocaine forced abstinence on the composition of the NMDA receptor subunits and on the levels of active zone proteins, i.e., Ras-related protein 3A (Rab3A), Rab3 interacting molecules 1 (RIM1) and mammalian uncoordinated protein 13 (Munc13) in the rat nucleus accumbens.

Fri Jul 31 00:00:00 EDT 2020

Cocaine Self-Administration and Abstinence Modulate NMDA Receptor Subunits and Active Zone Proteins in the Rat Nucleus Accumbens.

Molecules (Basel, Switzerland)

Smaga I,Wydra K,Frankowska M,Fumagalli F,Sanak M,Filip M

Fri Jul 31 00:00:00 EDT 2020

LAR receptor phospho-tyrosine phosphatases regulate NMDA-receptor responses.

eLife

Sclip A,Südhof TC

A-6473 was used in Western Blot to test the synaptogenic functions of LAR-RPTPs, by conditionally deleting the genes encoding all three LAR-RPTPs, singly or in combination, in mice before synapse formation.

Mon Jan 27 00:00:00 EST 2020

LAR receptor phospho-tyrosine phosphatases regulate NMDA-receptor responses.

eLife

Sclip A,Südhof TC

Mon Jan 27 00:00:00 EST 2020

Development and Characterization of Human Cerebral Organoids: An Optimized Protocol.

Cell transplantation

Yakoub AM,Sadek M

A-6473 was used in Western Blotting to develop an optimised methodology for the growth of organoids, and define the necessary and sufficient factors that support their optimal development.

Thu Mar 01 00:00:00 EST 2018

Homocysteine sensitizes the mouse neuromuscular junction to oxidative stress by nitric oxide.

Neuroreport

Wang JS,Bojovic D,Chen Y,Lindgren CA

A-6473 was used in Immunohistochemistry-immunofluorescence to show homocysteine acts synergistically with ROS to induce significant decreases in neurotransmission at the NMJ.

Wed Aug 15 00:00:00 EDT 2018

Homocysteine sensitizes the mouse neuromuscular junction to oxidative stress by nitric oxide.

Neuroreport

Wang JS,Bojovic D,Chen Y,Lindgren CA

A-6473 was used in Immunohistochemistry-immunofluorescence to show homocysteine acts synergistically with ROS to induce significant decreases in neurotransmission at the NMJ.

Wed Aug 15 00:00:00 EDT 2018

Glutamate Deregulation in Ketamine-Induced Psychosis-A Potential Role of PSD95, NMDA Receptor and PMCA Interaction.

Frontiers in cellular neuroscience

Lisek M,Ferenc B,Studzian M,Pulaski L,Guo F,Zylinska L,Boczek T

Published figure using NMDAR2A polyclonal antibody (Product # A-6473) in Western Blot

Thu Oct 01 00:00:00 EDT 2020

Mechanisms of Functional Hypoconnectivity in the Medial Prefrontal Cortex of Mecp2 Null Mice.

Cerebral cortex (New York, N.Y. : 1991)

Sceniak MP,Lang M,Enomoto AC,James Howell C,Hermes DJ,Katz DM

A-6473 was used in western blot to investigate how the loss of Mecp2 disrupts function in the murine medial prefrontal cortex using acute brain slices and behavioral testing.

Sun May 01 00:00:00 EDT 2016

SALM4 suppresses excitatory synapse development by cis-inhibiting trans-synaptic SALM3-LAR adhesion.

Nature communications

Lie E,Ko JS,Choi SY,Roh JD,Cho YS,Noh R,Kim D,Li Y,Kang H,Choi TY,Nam J,Mah W,Lee D,Lee SG,Kim HM,Kim H,Choi SY,Um JW,Kang MG,Bae YC,Ko J,Kim E

A-6473 was used in western blot propose that SALM4 negatively regulates excitatory synapses via inhibition of SALM3-LAR adhesion

Tue Aug 02 00:00:00 EDT 2016

Chronic copper exposure causes spatial memory impairment, selective loss of hippocampal synaptic proteins, and activation of PKR/eIF2α pathway in mice.

Journal of Alzheimer's disease : JAD

Ma Q,Ying M,Sui X,Zhang H,Huang H,Yang L,Huang X,Zhuang Z,Liu J,Yang X

A-6473 was used in western blot to study the effect of copper exposure on memory

Tue Aug 18 00:00:00 EDT 2015

PSD95 suppresses dendritic arbor development in mature hippocampal neurons by occluding the clustering of NR2B-NMDA receptors.

PloS one

Bustos FJ,Varela-Nallar L,Campos M,Henriquez B,Phillips M,Opazo C,Aguayo LG,Montecino M,Constantine-Paton M,Inestrosa NC,van Zundert B

A-6473 was used in immunocytochemistry and western blot to study the roles of the NR2A and NR2B subunits, and of their scaffolding proteins, in remodeling the dendritic architecture of developing hippocampal neurons

Wed Dec 03 00:00:00 EST 2014

PSD95 suppresses dendritic arbor development in mature hippocampal neurons by occluding the clustering of NR2B-NMDA receptors.

PloS one

Bustos FJ,Varela-Nallar L,Campos M,Henriquez B,Phillips M,Opazo C,Aguayo LG,Montecino M,Constantine-Paton M,Inestrosa NC,van Zundert B

Wed Dec 03 00:00:00 EST 2014

Estradiol potentiation of NR2B-dependent EPSCs is not due to changes in NR2B protein expression or phosphorylation.

Hippocampus

Snyder MA,Cooke BM,Woolley CS

A-6473 was used in western blot to investigate the mechanism for the synaptic regulation by estradiol

Fri Apr 01 00:00:00 EDT 2011

Antibody-mediated targeted gene transfer of helper virus-free HSV-1 vectors to rat neocortical neurons that contain either NMDA receptor 2B or 2A subunits.

Brain research

Cao H,Zhang GR,Geller AI

A-6473 was used in immunohistochemistry to investigate antibody-mediated targeted gene transfer.

Fri Sep 30 00:00:00 EDT 2011

Cellular and synaptic mechanisms of anti-NMDA receptor encephalitis.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Hughes EG,Peng X,Gleichman AJ,Lai M,Zhou L,Tsou R,Parsons TD,Lynch DR,Dalmau J,Balice-Gordon RJ

A-6473 was used in western blot to investigate the effects of autoantibodies to the NMDA receptor

Wed Apr 28 00:00:00 EDT 2010

Cellular and synaptic mechanisms of anti-NMDA receptor encephalitis.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Hughes EG,Peng X,Gleichman AJ,Lai M,Zhou L,Tsou R,Parsons TD,Lynch DR,Dalmau J,Balice-Gordon RJ

A-6473 was used in western blot to investigate the effects of autoantibodies to the NMDA receptor

Wed Apr 28 00:00:00 EDT 2010

Neto1 is a novel CUB-domain NMDA receptor-interacting protein required for synaptic plasticity and learning.

PLoS biology

Ng D,Pitcher GM,Szilard RK,Sertié A,Kanisek M,Clapcote SJ,Lipina T,Kalia LV,Joo D,McKerlie C,Cortez M,Roder JC,Salter MW,McInnes RR

Published figure using NMDAR2A polyclonal antibody (Product # A-6473) in Western Blot

Tue Feb 24 00:00:00 EST 2009

Neuronal interleukin-16 (NIL-16): a dual function PDZ domain protein.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Kurschner C,Yuzaki M

Published figure using NMDAR2A polyclonal antibody (Product # A-6473) in Immunoprecipitation

Wed Sep 15 00:00:00 EDT 1999

Bioinformatics

Protein Aliases: GluN2A; GluRepsilon1; Glutamate [NMDA] receptor subunit epsilon-1; glutamate receptor; Glutamate receptor ionotropic, NMDA 2A; glutamate receptor, ionotropic, N-methyl D-aspartate 2A; hNR2A; N-methyl D-aspartate receptor subtype 2A; N-methyl-D-aspartate receptor channel, subunit epsilon-1; N-methyl-D-aspartate receptor subunit 2A; NMDA Receptor 2A; NMDAR 2A; NMDAR2A; OTTHUMP00000160135; OTTHUMP00000174531

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Gene Aliases: EPND; FESD; GluN2A; GRIN2A; LKS; NMDAR2A; NR2A

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UniProt ID: (Human) Q12879, (Mouse) P35436, (Rat) Q00959

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Entrez Gene ID: (Human) 2903, (Mouse) 14811, (Rat) 24409

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Function(s)

NMDA glutamate receptor activity Ras guanyl-nucleotide exchange factor activity extracellular-glutamate-gated ion channel activity calcium channel activity protein binding zinc ion binding receptor activity ionotropic glutamate receptor activity receptor binding ion channel activity cation channel activity glutamate binding protein kinase binding voltage-gated cation channel activity protein complex binding glutamate receptor binding neurotransmitter binding metal ion binding protein heterodimerization activity protein dimerization activity cell adhesion molecule binding ATPase binding scaffold protein binding transmembrane signal receptor

Process(es)

MAPK cascade startle response response to amphetamine transport glutamate receptor signaling pathway chemical synaptic transmission learning or memory memory protein localization visual learning response to wounding sensory perception of pain neurogenesis sleep directional locomotion ionotropic glutamate receptor signaling pathway negative regulation of protein catabolic process dopamine metabolic process serotonin metabolic process response to drug positive regulation of apoptotic process positive regulation of GTPase activity response to ethanol regulation of sensory perception of pain excitatory postsynaptic potential long-term synaptic potentiation calcium ion transmembrane transport positive regulation of long-term synaptic potentiation action potential ion transport cation transport calcium ion transport learning locomotory behavior positive regulation of cell death ion transmembrane transport regulation of ion transmembrane transport synaptic transmission, glutamatergic locomotion regulation of membrane potential regulation of synaptic plasticity regulation of long-term neuronal synaptic plasticity rhythmic process modulation of synaptic transmission detection of mechanical stimulus involved in sensory perception of pain protein tetramerization regulation of postsynaptic membrane potential cellular response to amino acid stimulus cellular response to zinc ion cation transmembrane transport positive regulation of excitatory postsynaptic potential response to light stimulus response to carbohydrate response to manganese ion response to organic cyclic compound response to amine spinal cord development hippocampus development cerebral cortex development response to cocaine response to calcium ion response to methylmercury response to other organism response to ammonium ion response to fungicide cellular response to magnesium ion cellular response to manganese ion cellular response to dsRNA cellular response to growth factor stimulus cellular response to lipid

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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NMDAR2A Polyclonal Antibody

NMDAR2A Antibody (A-6473) in IHC (F)

Product Details

A-6473

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Immunoprecipitation (IP)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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