Invitrogen

NMDAR2B Polyclonal Antibody

NMDAR2B Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 21

NMDAR2B Antibody (71-8600) in ICC/IF

Immunofluorescence analysis of NMDA RECEPTOR 2B was performed using 70% confluent log phase RSC96 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with NMDAR2B Rabbit Polyclonal Antibody (Product # 71-8600) at 2µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate ... View More

NMDAR2B Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

NMDAR2B Antibody in Immunohistochemistry (IHC)

Product Image Gallery

Product Details

71-8600

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

View 17 publications 17 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

Immunohistochemistry - Free Floating (IHC (Free))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

2 µg/mL

View 4 publications 4 publications

Immunoprecipitation (IP)

View 3 publications 3 publications

Neutralization (Neu)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Rabbit, Rat

Published species

Dog, Human, Mouse, Non-human primate, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Fusion protein including a 251 amino acid sequence from the N-terminal portion of the rat NMDAR 2B protein.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2534001

Target Information

NMDAR2B is a member of the N-methyl-D-aspartate (NMDA) class of ionotropic glutamate receptors. NMDA receptors (NMDAR) have been shown to be involved in long-term potentiation, an activity-dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning. NMDAR play a role in neuronal development and has been implicated in several disorders of the central nervous system including Alzheimer’s Disease, epilepsy and ischemic neuronal cell death. Overexpression of the NR2B subunit of the receptor has been associated with increases in learning and memory while aged, memory impaired animals have deficiencies in NR2B expression. Tyr1472 on NR2B is phosphorylated and this may lead to the increased expression of the NMDAR at the synapse that plays a role in synaptic plasticity. NMDR2B is a heterotrimer comprised of seven enetically encoded and differentially expressed subunits that include NR1, NR2 and NR3. Diseases associated with the dysfunction of NMDAR2B include epileptic encephalopathy, autosomal dominant 6-related mental retardation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Detrimental effects of soluble α-synuclein oligomers at excitatory glutamatergic synapses.

Frontiers in aging neuroscience

Ferrari E,Salvadè M,Zianni E,Brumana M,DiLuca M,Gardoni F

Published figure using NMDAR2B polyclonal antibody (Product # 71-8600) in Western Blot

Tue Apr 04 00:00:00 EDT 2023

Synaptic mechanisms underlying onset and progression of memory deficits caused by hippocampal and midbrain synucleinopathy.

NPJ Parkinson's disease

Iemolo A,De Risi M,Giordano N,Torromino G,Somma C,Cavezza D,Colucci M,Mancini M,de Iure A,Granata R,Picconi B,Calabresi P,De Leonibus E

71-8600 was used in Western Blotting to identify the synaptic mechanisms leading to memory impairment induced by hippocampal α-synucleinopathy and provide functional evidence of the major neuronal networks involved in disease progression.

Fri Jun 16 00:00:00 EDT 2023

Rabphilin-3A as a novel target to reverse α-synuclein-induced synaptic loss in Parkinson's disease.

Pharmacological research

Ferrari E,Scheggia D,Zianni E,Italia M,Brumana M,Palazzolo L,Parravicini C,Pilotto A,Padovani A,Marcello E,Eberini I,Calabresi P,Diluca M,Gardoni F

71-8600 was used in Immunocytochemistry, Immunohistochemistry (Frozen), Western Blot to indicate that approaches aimed at restoring Rabphilin-3A (Rph3A) synaptic functions can slow down the early synaptic detrimental effects of α-synuclein aggregates in Parkinson's disease.

Thu Sep 01 00:00:00 EDT 2022

Functional NMDA receptors are expressed by human pulmonary artery smooth muscle cells.

Scientific reports

Dong YN,Hsu FC,Koziol-White CJ,Stepanova V,Jude J,Gritsiuta A,Rue R,Mott R,Coulter DA,Panettieri RA,Krymskaya VP,Takano H,Goncharova EA,Goncharov DA,Cines DB,Lynch DR

Published figure using NMDAR2B polyclonal antibody (Product # 71-8600) in Immunocytochemistry

Thu Apr 15 00:00:00 EDT 2021

Noradrenergic projections from the locus coeruleus to the amygdala constrain fear memory reconsolidation.

eLife

Haubrich J,Bernabo M,Nader K

71-8600 was used in Western Blotting to investigate the hypothesis that specific modulatory signals shape memory formation into a state that is reconsolidation-resistant.

Mon May 18 00:00:00 EDT 2020

Linking NMDA Receptor Synaptic Retention to Synaptic Plasticity and Cognition.

iScience

Franchini L,Stanic J,Ponzoni L,Mellone M,Carrano N,Musardo S,Zianni E,Olivero G,Marcello E,Pittaluga A,Sala M,Bellone C,Racca C,Di Luca M,Gardoni F

71-8600 was used in Western Blotting to induce synaptic plasticity in the presence or absence of agents modulating Rph3A function.

Fri Sep 27 00:00:00 EDT 2019

The Developmental Shift of NMDA Receptor Composition Proceeds Independently of GluN2 Subunit-Specific GluN2 C-Terminal Sequences.

Cell reports

McKay S,Ryan TJ,McQueen J,Indersmitten T,Marwick KFM,Hasel P,Kopanitsa MV,Baxter PS,Martel MA,Kind PC,Wyllie DJA,O'Dell TJ,Grant SGN,Hardingham GE,Komiyama NH

71-8600 was used in Immunoprecipitation to study the developmental changes in NMDA receptor composition, and the roles of GluN2A expression levels and GluN2 subtype-specific CTD-driven events in these changes.

Tue Oct 23 00:00:00 EDT 2018

The Developmental Shift of NMDA Receptor Composition Proceeds Independently of GluN2 Subunit-Specific GluN2 C-Terminal Sequences.

Cell reports

McKay S,Ryan TJ,McQueen J,Indersmitten T,Marwick KFM,Hasel P,Kopanitsa MV,Baxter PS,Martel MA,Kind PC,Wyllie DJA,O'Dell TJ,Grant SGN,Hardingham GE,Komiyama NH

Tue Oct 23 00:00:00 EDT 2018

The Developmental Shift of NMDA Receptor Composition Proceeds Independently of GluN2 Subunit-Specific GluN2 C-Terminal Sequences.

Cell reports

McKay S,Ryan TJ,McQueen J,Indersmitten T,Marwick KFM,Hasel P,Kopanitsa MV,Baxter PS,Martel MA,Kind PC,Wyllie DJA,O'Dell TJ,Grant SGN,Hardingham GE,Komiyama NH

Tue Oct 23 00:00:00 EDT 2018

The Developmental Shift of NMDA Receptor Composition Proceeds Independently of GluN2 Subunit-Specific GluN2 C-Terminal Sequences.

Cell reports

McKay S,Ryan TJ,McQueen J,Indersmitten T,Marwick KFM,Hasel P,Kopanitsa MV,Baxter PS,Martel MA,Kind PC,Wyllie DJA,O'Dell TJ,Grant SGN,Hardingham GE,Komiyama NH

Tue Oct 23 00:00:00 EDT 2018

Timing-dependent LTP and LTD in mouse primary visual cortex following different visual deprivation models.

PloS one

Guo Y,Zhang W,Chen X,Fu J,Cheng W,Song D,Qu X,Yang Z,Zhao K

71-8600 was used in Western Blotting to demonstrate the temporal window changes of timing-dependent long-term potentiation (LTP) and long-term depression (LTD), elicited by coincidental pre- and post-synaptic firing, following 6-day visual deprivation.

Mon Sep 11 00:00:00 EDT 2017

Stereotactic injection of cerebrospinal fluid from anti-NMDA receptor encephalitis into rat dentate gyrus impairs NMDA receptor function.

Brain research

Würdemann T,Kersten M,Tokay T,Guli X,Kober M,Rohde M,Porath K,Sellmann T,Bien CG,Köhling R,Kirschstein T

71-8600 was used in blocking or activating experiment to determine impairment of NMDA receptor function by sterotactic injection of cerebrospinal fluid from anti-NMDA receptor encephalitis into rat dentate gyrus

Mon Feb 15 00:00:00 EST 2016

NMDA receptors are selectively partitioned into complexes and supercomplexes during synapse maturation.

Nature communications

Frank RA,Komiyama NH,Ryan TJ,Zhu F,O'Dell TJ,Grant SG

71-8600 was used in western blot to analyze selective partitioning into complexes and supercomplexes during synapse maturation by NMDA receptors

Wed Apr 27 00:00:00 EDT 2016

Defective Age-Dependent Metaplasticity in a Mouse Model of Alzheimer's Disease.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Megill A,Tran T,Eldred K,Lee NJ,Wong PC,Hoe HS,Kirkwood A,Lee HK

71-8600 was used in western blot to test if LTP/LTD changes in Alzheimer's disease mouse models indicate defects in metaplasticity processes

Wed Aug 12 00:00:00 EDT 2015

Evolution of GluN2A/B cytoplasmic domains diversified vertebrate synaptic plasticity and behavior.

Nature neuroscience

Ryan TJ,Kopanitsa MV,Indersmitten T,Nithianantharajah J,Afinowi NO,Pettit C,Stanford LE,Sprengel R,Saksida LM,Bussey TJ,O'Dell TJ,Grant SG,Komiyama NH

Published figure using NMDAR2B polyclonal antibody (Product # 71-8600) in Western Blot

Tue Jan 01 00:00:00 EST 2013

A tetra(ethylene glycol) derivative of benzothiazole aniline enhances Ras-mediated spinogenesis.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Megill A,Lee T,DiBattista AM,Song JM,Spitzer MH,Rubinshtein M,Habib LK,Capule CC,Mayer M,Turner RS,Kirkwood A,Yang J,Pak DT,Lee HK,Hoe HS

71-8600 was used in western blot to study the effect of a novel amyloid-binding small molecule on Ras-mediated spinogenesis

Wed May 29 00:00:00 EDT 2013

ALLN rescues an in vitro excitatory synaptic transmission deficit in Lis1 mutant mice.

Journal of neurophysiology

Sebe JY,Bershteyn M,Hirotsune S,Wynshaw-Boris A,Baraban SC

Published figure using NMDAR2B polyclonal antibody (Product # 71-8600) in Western Blot

Tue Jan 01 00:00:00 EST 2013

Allopregnanolone prevents dieldrin-induced NMDA receptor internalization and neurotoxicity by preserving GABA(A) receptor function.

Endocrinology

Briz V,Parkash J,Sánchez-Redondo S,Prevot V,Suñol C

71-8600 was used in Immunoprecipitation to show that allopregnanolone protects cortical neurons against the neurotoxicity caused by long-term exposure to dieldrin by maintaining γ-aminobutyric acid A receptor and N-methyl-D-aspartate receptor functionality.

Wed Feb 01 00:00:00 EST 2012

Phosphorylation of N-methyl-D-aspartic acid receptor-associated neuronal nitric oxide synthase depends on estrogens and modulates hypothalamic nitric oxide production during the ovarian cycle.

Endocrinology

Parkash J,d'Anglemont de Tassigny X,Bellefontaine N,Campagne C,Mazure D,Buée-Scherrer V,Prevot V

71-8600 was used in immunohistochemistry to investigate the relationship between neuronal NO synthase and estrogen.

Tue Jun 01 00:00:00 EDT 2010

Estradiol induces physical association of neuronal nitric oxide synthase with NMDA receptor and promotes nitric oxide formation via estrogen receptor activation in primary neuronal cultures.

Journal of neurochemistry

d'Anglemont de Tassigny X,Campagne C,Steculorum S,Prevot V

Published figure using NMDAR2B polyclonal antibody (Product # 71-8600) in Immunocytochemistry

Wed Apr 01 00:00:00 EDT 2009

Estradiol induces physical association of neuronal nitric oxide synthase with NMDA receptor and promotes nitric oxide formation via estrogen receptor activation in primary neuronal cultures.

Journal of neurochemistry

d'Anglemont de Tassigny X,Campagne C,Steculorum S,Prevot V

Published figure using NMDAR2B polyclonal antibody (Product # 71-8600) in Immunocytochemistry

Wed Apr 01 00:00:00 EDT 2009

Polyglutamine-modulated striatal calpain activity in YAC transgenic huntington disease mouse model: impact on NMDA receptor function and toxicity.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Cowan CM,Fan MM,Fan J,Shehadeh J,Zhang LY,Graham RK,Hayden MR,Raymond LA

71-8600 was used in western blot to report that the cumulative effect of increasing huntingtin polyglutamine length enhancement of medium-sized spiny neuron sensitivity to excitotoxicity

Wed Nov 26 00:00:00 EST 2008

Differential trafficking of AMPA and NMDA receptors during long-term potentiation in awake adult animals.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Williams JM,Guévremont D,Mason-Parker SE,Luxmanan C,Tate WP,Abraham WC

Wed Dec 19 00:00:00 EST 2007

Human immunodeficiency virus (HIV)-induced neurotoxicity: roles for the NMDA receptor subtypes.

The Journal of neuroscience : the official journal of the Society for Neuroscience

O'Donnell LA,Agrawal A,Jordan-Sciutto KL,Dichter MA,Lynch DR,Kolson DL

Published figure using NMDAR2B polyclonal antibody (Product # 71-8600) in Western Blot

Wed Jan 18 00:00:00 EST 2006

N-methyl-D-aspartate receptor subunit phenotypes of vagal afferent neurons in nodose ganglia of the rat.

The Journal of comparative neurology

Czaja K,Ritter RC,Burns GA

Published figure using NMDAR2B polyclonal antibody (Product # 71-8600) in Immunofluorescence

Tue Jun 20 00:00:00 EDT 2006

N-methyl-D-aspartate receptor subunit phenotypes of vagal afferent neurons in nodose ganglia of the rat.

The Journal of comparative neurology

Czaja K,Ritter RC,Burns GA

Published figure using NMDAR2B polyclonal antibody (Product # 71-8600) in Immunofluorescence

Tue Jun 20 00:00:00 EDT 2006

Distribution of glutamate receptor subunits in experimentally induced cortical malformations.

Neuroscience

Hagemann G,Kluska MM,Redecker C,Luhmann HJ,Witte OW

71-8600 was used in immunohistochemistry - free floating to examine the regional expression of four NMDA receptor subunits and two major AMPA- and kainate-receptor complexes in adult rats after neonatal freeze lesions.

Tue Jun 10 00:00:00 EDT 2003

Long-term regulation of N-methyl-D-aspartate receptor subunits and associated synaptic proteins following hippocampal synaptic plasticity.

Neuroscience

Williams JM,Guévremont D,Kennard JT,Mason-Parker SE,Tate WP,Abraham WC

Fri Jul 11 00:00:00 EDT 2003

Dual palmitoylation of PSD-95 mediates its vesiculotubular sorting, postsynaptic targeting, and ion channel clustering.

The Journal of cell biology

El-Husseini AE,Craven SE,Chetkovich DM,Firestein BL,Schnell E,Aoki C,Bredt DS

Mon Jan 10 00:00:00 EST 2000

Dual palmitoylation of PSD-95 mediates its vesiculotubular sorting, postsynaptic targeting, and ion channel clustering.

The Journal of cell biology

El-Husseini AE,Craven SE,Chetkovich DM,Firestein BL,Schnell E,Aoki C,Bredt DS

Mon Jan 10 00:00:00 EST 2000

Dual palmitoylation of PSD-95 mediates its vesiculotubular sorting, postsynaptic targeting, and ion channel clustering.

The Journal of cell biology

El-Husseini AE,Craven SE,Chetkovich DM,Firestein BL,Schnell E,Aoki C,Bredt DS

Mon Jan 10 00:00:00 EST 2000

Dual palmitoylation of PSD-95 mediates its vesiculotubular sorting, postsynaptic targeting, and ion channel clustering.

The Journal of cell biology

El-Husseini AE,Craven SE,Chetkovich DM,Firestein BL,Schnell E,Aoki C,Bredt DS

Mon Jan 10 00:00:00 EST 2000

PSD-95 assembles a ternary complex with the N-methyl-D-aspartic acid receptor and a bivalent neuronal NO synthase PDZ domain.

The Journal of biological chemistry

Christopherson KS,Hillier BJ,Lim WA,Bredt DS

Fri Sep 24 00:00:00 EDT 1999

Bioinformatics

Protein Aliases: GluN2B; Glutamate [NMDA] receptor subunit epsilon-2; glutamate receptor; Glutamate receptor ionotropic, NMDA 2B; glutamate receptor, ionotropic, N-methyl D-aspartate 2B; glutamate receptor, ionotropic, NMDA2B; GRIN 2B; HGNC:4586; hNR 3; MGC142178; MGC142180; N-methyl D-aspartate receptor subtype 2B; NMDAR2B; NMDE2; NME2; NR2B

View more View less

Gene Aliases: GluN2B; Grin2b

View more View less

UniProt ID: (Rat) Q00960

View more View less

Entrez Gene ID: (Rabbit) 100353266, (Rat) 24410

View more View less

Function(s)

ionotropic glutamate receptor activity NMDA glutamate receptor activity receptor binding interleukin-1 receptor binding extracellular-glutamate-gated ion channel activity cation channel activity calcium channel activity protein binding beta-catenin binding drug binding zinc ion binding glycine binding D2 dopamine receptor binding ionotropic glutamate receptor binding neurotransmitter binding protein heterodimerization activity cell adhesion molecule binding transmembrane signal receptor

Process(es)

action potential behavioral fear response in utero embryonic development startle response suckling behavior response to amphetamine cation transport calcium ion transport chemical synaptic transmission sensory organ development learning or memory learning memory long-term memory associative learning response to mechanical stimulus response to toxic substance response to carbohydrate response to manganese ion cellular response to magnesium starvation regulation of protein kinase A signaling positive regulation of cell death positive regulation of glutamate secretion response to organic cyclic compound response to amine hippocampus development cerebral cortex development response to magnesium ion multicellular organismal response to stress response to cytokine ion transmembrane transport ionotropic glutamate receptor signaling pathway synaptic transmission, glutamatergic response to cocaine fear response receptor clustering regulation of MAPK cascade response to ethanol regulation of synaptic plasticity regulation of long-term neuronal synaptic plasticity behavioral response to pain rhythmic process positive regulation of synaptic transmission detection of mechanical stimulus involved in sensory perception of pain response to calcium ion response to methylmercury response to electrical stimulus response to other organism regulation of postsynaptic membrane potential excitatory postsynaptic potential long-term synaptic potentiation response to growth hormone response to fungicide calcium ion transmembrane transport cellular response to amino acid stimulus cellular response to manganese ion cellular response to dsRNA cellular response to growth factor stimulus cellular response to lipid cellular response to organic cyclic compound cation transmembrane transport

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

NMDAR2B Polyclonal Antibody

NMDAR2B Antibody (71-8600) in ICC/IF

Product Details

71-8600

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Frozen) (IHC (F))

Immunohistochemistry - Free Floating (IHC (Free))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Neutralization (Neu)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!