Invitrogen

NMDAR2B Polyclonal Antibody

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NMDAR2B Antibody (A-6474) in WB

Western blot was performed using NMDAR2B Polyclonal Antibody (Product # A-6474) and a 166 kDa and 170 kDa band corresponding to NMDAR2B were observed across tissues tested except for mouse liver and heart, which are the low expressors for NMDAR2B. Whole cell extracts (30 µg lysate) of Mouse Brain (Lane 1), Mouse Pup Brain (Lane 2), Mouse Cerebellum (Lane 3), Mouse Liver (Lane 4), Mouse Heart (Lane 5) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX), 10 well. Resolved proteins were then transferred onto a ... View More

NMDAR2B Antibody in Immunocytochemistry (ICC/IF)

NMDAR2B Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

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Product Details

A-6474

Applications

Tested Dilution

Publications

Western Blot (WB)

1:100-1:2,000

View 8 publications 8 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

1:500-1:5,000

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

ELISA (ELISA)

Assay-dependent

Immunoprecipitation (IP)

3 µL

Miscellaneous PubMed (Misc)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Fusion protein containing amino acid residues 984-1104 of the C-terminal region of rat brain subunit 2B

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

IgG fraction

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2536210

Product Specific Information

This antibody is specific for the ~180 kDa 2B subunit from rat, mouse and human.

Storage and reconstitution: when stored at -20ºC or below, the lyophilized antibody should retain full activity for over one year. Reconstitute using 50 µL PBS, pH 7.4, to yield a 0.2 mg/mL solution. If storing at 0-4ºC, add sodium azide to a final concentration of 2mM. For longer storage, divide the solution into aliquots and freeze at -20ºC, avoiding freeze/thaw cycles.

Target Information

NMDAR2B is a member of the N-methyl-D-aspartate (NMDA) class of ionotropic glutamate receptors. NMDA receptors (NMDAR) have been shown to be involved in long-term potentiation, an activity-dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning. NMDAR play a role in neuronal development and has been implicated in several disorders of the central nervous system including Alzheimer’s Disease, epilepsy and ischemic neuronal cell death. Overexpression of the NR2B subunit of the receptor has been associated with increases in learning and memory while aged, memory impaired animals have deficiencies in NR2B expression. Tyr1472 on NR2B is phosphorylated and this may lead to the increased expression of the NMDAR at the synapse that plays a role in synaptic plasticity. NMDR2B is a heterotrimer comprised of seven enetically encoded and differentially expressed subunits that include NR1, NR2 and NR3. Diseases associated with the dysfunction of NMDAR2B include epileptic encephalopathy, autosomal dominant 6-related mental retardation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Loss of spines in the prelimbic cortex is detrimental to working memory in mice with early-life adversity.

Molecular psychiatry

Xu L,Liu Y,Long J,He X,Xie F,Yin Q,Chen M,Long D,Chen Y

A-6474 was used in Western Blotting to investigate the impact of early-life adversity (ELA) on dendritic spines, postsynaptic puncta, and glutamatergic transmission in the prelimbic cortex, finding that ELA results in the loss of spines, postsynaptic puncta, and depressed glutamatergic transmission, which contributes to poor spatial working memory, and exciting Thy1-expressing PrL pyramidal cells can improve working memory in ELA mice.

Tue Aug 01 00:00:00 EDT 2023

Exo70 intracellular redistribution after repeated mild traumatic brain injury.

Biological research

Lira M,Zamorano P,Cerpa W

A-6474 was used in Western Blot, Immunohistochemistry to position Exo70 in the group of proteins that could modulate GluN2B synaptic availability in acute neuropathology like a traumatic brain injury.

Tue Feb 16 00:00:00 EST 2021

Exo70 intracellular redistribution after repeated mild traumatic brain injury.

Biological research

Lira M,Zamorano P,Cerpa W

Tue Feb 16 00:00:00 EST 2021

Effects of RORγt overexpression on the murine central nervous system.

Neuropsychopharmacology reports

Sasaki T,Nagata R,Takahashi S,Takei Y

A-6474 was used in Western Blot to study changes in the brain caused by chronically increased IL-17A resulting from excessive activation of Th17 cells, due to overexpressing RORγt, a transcription factor essential for differentiation of Th17 cells (RORγt Tg mice).

Mon Mar 01 00:00:00 EST 2021

Effects of RORγt overexpression on the murine central nervous system.

Neuropsychopharmacology reports

Sasaki T,Nagata R,Takahashi S,Takei Y

Mon Mar 01 00:00:00 EST 2021

Interfering of the Reelin/ApoER2/PSD95 Signaling Axis Reactivates Dendritogenesis of Mature Hippocampal Neurons.

Journal of cellular physiology

Ampuero E,Jury N,Härtel S,Marzolo MP,van Zundert B

A-6474 was used in immunocytochemistry to test if impairment of reelin signaling in adult neurons reactivates the dendritic architecture

Mon May 01 00:00:00 EDT 2017

Mechanisms of Functional Hypoconnectivity in the Medial Prefrontal Cortex of Mecp2 Null Mice.

Cerebral cortex (New York, N.Y. : 1991)

Sceniak MP,Lang M,Enomoto AC,James Howell C,Hermes DJ,Katz DM

A-6474 was used in western blot to investigate how the loss of Mecp2 disrupts function in the murine medial prefrontal cortex using acute brain slices and behavioral testing.

Sun May 01 00:00:00 EDT 2016

Chronic copper exposure causes spatial memory impairment, selective loss of hippocampal synaptic proteins, and activation of PKR/eIF2α pathway in mice.

Journal of Alzheimer's disease : JAD

Ma Q,Ying M,Sui X,Zhang H,Huang H,Yang L,Huang X,Zhuang Z,Liu J,Yang X

A-6474 was used in western blot to study the effect of copper exposure on memory

Tue Aug 18 00:00:00 EDT 2015

PSD95 suppresses dendritic arbor development in mature hippocampal neurons by occluding the clustering of NR2B-NMDA receptors.

PloS one

Bustos FJ,Varela-Nallar L,Campos M,Henriquez B,Phillips M,Opazo C,Aguayo LG,Montecino M,Constantine-Paton M,Inestrosa NC,van Zundert B

A-6474 was used in immunocytochemistry and western blot to study the roles of the NR2A and NR2B subunits, and of their scaffolding proteins, in remodeling the dendritic architecture of developing hippocampal neurons

Wed Dec 03 00:00:00 EST 2014

PSD95 suppresses dendritic arbor development in mature hippocampal neurons by occluding the clustering of NR2B-NMDA receptors.

PloS one

Bustos FJ,Varela-Nallar L,Campos M,Henriquez B,Phillips M,Opazo C,Aguayo LG,Montecino M,Constantine-Paton M,Inestrosa NC,van Zundert B

Wed Dec 03 00:00:00 EST 2014

Pre- and postsynaptic localization of NMDA receptor subunits at hippocampal mossy fibre synapses.

Neuroscience

Berg LK,Larsson M,Morland C,Gundersen V

A-6474 was used in immunohistochemistry to determine the subcellular localization of N-methyl-D-aspartate type of glutamate receptors subunits in the hippocampal mossy fibre-CA3 pyramidal neuron synapses.

Tue Jan 29 00:00:00 EST 2013

Cholinergic dysfunction alters synaptic integration between thalamostriatal and corticostriatal inputs in DYT1 dystonia.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Sciamanna G,Tassone A,Mandolesi G,Puglisi F,Ponterio G,Martella G,Madeo G,Bernardi G,Standaert DG,Bonsi P,Pisani A

A-6474 was used in immunohistochemistry and western blot to study synaptic integration between thalamostriatal and corticostriatal inputs.

Wed Aug 29 00:00:00 EDT 2012

Subunit-specific modulation of [(3)H]MK-801 binding to NMDA receptors mediated by dopamine receptor ligands in rodent brain.

Neurochemistry international

Wigestrand MB,Fonnum F,Ivar Walaas S

A-6474 was used in western blot to identify DR ligands that directly modulate NMDARs.

Sun Jul 01 00:00:00 EDT 2012

Neurotoxic lupus autoantibodies alter brain function through two distinct mechanisms.

Proceedings of the National Academy of Sciences of the United States of America

Faust TW,Chang EH,Kowal C,Berlin R,Gazaryan IG,Bertini E,Zhang J,Sanchez-Guerrero J,Fragoso-Loyo HE,Volpe BT,Diamond B,Huerta PT

A-6474 was used in immunoprecipitation to study the cellular mechanisms of damage induced by NMDAR-reactive autoantibodies

Tue Oct 26 00:00:00 EDT 2010

Regulation of NMDA receptor subunits and nitric oxide synthase expression during cocaine withdrawal.

Journal of neurochemistry

Loftis JM,Janowsky A

A-6474 was used in immunohistochemistry - frozen section to determine the effects of cocaine withdrawal on the expression of NMDA receptor subunits and neuronal nitric oxide synthase

Wed Nov 01 00:00:00 EST 2000

Bioinformatics

Protein Aliases: GluN2B; GluRepsilon2; Glutamate [NMDA] receptor subunit epsilon-2; glutamate receptor; Glutamate receptor ionotropic, NMDA 2B; glutamate receptor subunit epsilon-2; glutamate receptor, ionotropic, N-methyl D-aspartate 2B; glutamate receptor, ionotropic, NMDA2B; GRIN 2B; HGNC:4586; hNR 3; MGC142178; MGC142180; N-methyl D-aspartate receptor 2B; N-methyl D-aspartate receptor subtype 2B; N-methyl-D-aspartate receptor subunit 3; NMDAR2B; NMDE2; NME2; NR2B; NR3

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Gene Aliases: AW490526; EIEE27; GluN2B; GRIN2B; hNR3; MRD6; NMDAR2B; NR2B

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UniProt ID: (Human) Q13224, (Mouse) Q01097, (Rat) Q00960

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Entrez Gene ID: (Human) 2904, (Mouse) 14812, (Rat) 24410

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Function(s)

NMDA glutamate receptor activity Ras guanyl-nucleotide exchange factor activity extracellular-glutamate-gated ion channel activity protein binding zinc ion binding glycine binding receptor activity ionotropic glutamate receptor activity receptor binding interleukin-1 receptor binding ion channel activity cation channel activity calcium channel activity beta-catenin binding drug binding D2 dopamine receptor binding ionotropic glutamate receptor binding neurotransmitter binding metal ion binding protein heterodimerization activity cell adhesion molecule binding transmembrane signal receptor

Process(es)

MAPK cascade transport glutamate receptor signaling pathway chemical synaptic transmission learning or memory ion transmembrane transport ionotropic glutamate receptor signaling pathway positive regulation of GTPase activity response to ethanol ephrin receptor signaling pathway action potential behavioral fear response in utero embryonic development startle response suckling behavior ion transport cation transport calcium ion transport sensory organ development learning memory associative learning embryo development regulation of protein kinase A signaling positive regulation of cell death positive regulation of glutamate secretion synaptic transmission, glutamatergic fear response receptor clustering regulation of MAPK cascade regulation of synaptic plasticity regulation of neuronal synaptic plasticity regulation of long-term neuronal synaptic plasticity behavioral response to pain rhythmic process positive regulation of synaptic transmission detection of mechanical stimulus involved in sensory perception of pain regulation of postsynaptic membrane potential excitatory postsynaptic potential long-term synaptic potentiation calcium ion transmembrane transport cation transmembrane transport response to amphetamine long-term memory response to mechanical stimulus response to toxic substance response to carbohydrate response to manganese ion cellular response to magnesium starvation response to organic cyclic compound response to amine hippocampus development cerebral cortex development response to magnesium ion multicellular organismal response to stress response to cytokine response to cocaine response to calcium ion response to methylmercury response to electrical stimulus response to other organism response to growth hormone response to fungicide cellular response to amino acid stimulus cellular response to manganese ion cellular response to dsRNA cellular response to growth factor stimulus cellular response to lipid cellular response to organic cyclic compound

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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NMDAR2B Polyclonal Antibody

NMDAR2B Antibody (A-6474) in WB

Product Details

A-6474

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Immunoprecipitation (IP)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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