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NMDAR2B Antibody (BS-0222R) in IHC (P)
Paraformaldehyde-fixed, paraffin embedded Rat Spinal Cord; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with NR2B Polyclonal Antibody, Unconjugated (bs-0222R) at 1:200 overnight at 4°C, DAB staining.
Product Details
BS-0222R
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
KLH conjugated synthetic peptide derived from human NMDAR2B, amino acids 451-550.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA
Contains
0.02% ProClin 300
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
NMDAR2B is a member of the N-methyl-D-aspartate (NMDA) class of ionotropic glutamate receptors. NMDA receptors (NMDAR) have been shown to be involved in long-term potentiation, an activity-dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning. NMDAR play a role in neuronal development and has been implicated in several disorders of the central nervous system including Alzheimer’s Disease, epilepsy and ischemic neuronal cell death. Overexpression of the NR2B subunit of the receptor has been associated with increases in learning and memory while aged, memory impaired animals have deficiencies in NR2B expression. Tyr1472 on NR2B is phosphorylated and this may lead to the increased expression of the NMDAR at the synapse that plays a role in synaptic plasticity. NMDR2B is a heterotrimer comprised of seven enetically encoded and differentially expressed subunits that include NR1, NR2 and NR3. Diseases associated with the dysfunction of NMDAR2B include epileptic encephalopathy, autosomal dominant 6-related mental retardation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: GluN2B; GluRepsilon2; Glutamate [NMDA] receptor subunit epsilon-2; glutamate receptor; Glutamate receptor ionotropic, NMDA 2B; glutamate receptor subunit epsilon-2; glutamate receptor, ionotropic, N-methyl D-aspartate 2B; glutamate receptor, ionotropic, NMDA2B; GRIN 2B; HGNC:4586; hNR 3; MGC142178; MGC142180; N-methyl D-aspartate receptor 2B; N-methyl D-aspartate receptor subtype 2B; N-methyl-D-aspartate receptor subunit 3; NMDAR2B; NMDE2; NME2; NR2B; NR3
Gene Aliases: AW490526; EIEE27; GluN2B; GRIN2B; hNR3; MRD6; NMDAR2B; NR2B
UniProt ID: (Human) Q13224, (Mouse) Q01097, (Rat) Q00960
Entrez Gene ID: (Human) 2904, (Mouse) 14812, (Rat) 24410
NMDA glutamate receptor activity
Ras guanyl-nucleotide exchange factor activity
extracellular-glutamate-gated ion channel activity
protein binding
zinc ion binding
glycine binding
receptor activity
ionotropic glutamate receptor activity
receptor binding
interleukin-1 receptor binding
ion channel activity
cation channel activity
calcium channel activity
beta-catenin binding
drug binding
D2 dopamine receptor binding
ionotropic glutamate receptor binding
neurotransmitter binding
metal ion binding
protein heterodimerization activity
cell adhesion molecule binding
transmembrane signal receptor
MAPK cascade
transport
glutamate receptor signaling pathway
chemical synaptic transmission
learning or memory
ion transmembrane transport
ionotropic glutamate receptor signaling pathway
positive regulation of GTPase activity
response to ethanol
ephrin receptor signaling pathway
action potential
behavioral fear response
in utero embryonic development
startle response
suckling behavior
ion transport
cation transport
calcium ion transport
sensory organ development
learning
memory
associative learning
embryo development
regulation of protein kinase A signaling
positive regulation of cell death
positive regulation of glutamate secretion
synaptic transmission, glutamatergic
fear response
receptor clustering
regulation of MAPK cascade
regulation of synaptic plasticity
regulation of neuronal synaptic plasticity
regulation of long-term neuronal synaptic plasticity
behavioral response to pain
rhythmic process
positive regulation of synaptic transmission
detection of mechanical stimulus involved in sensory perception of pain
regulation of postsynaptic membrane potential
excitatory postsynaptic potential
long-term synaptic potentiation
calcium ion transmembrane transport
cation transmembrane transport
response to amphetamine
long-term memory
response to mechanical stimulus
response to toxic substance
response to carbohydrate
response to manganese ion
cellular response to magnesium starvation
response to organic cyclic compound
response to amine
hippocampus development
cerebral cortex development
response to magnesium ion
multicellular organismal response to stress
response to cytokine
response to cocaine
response to calcium ion
response to methylmercury
response to electrical stimulus
response to other organism
response to growth hormone
response to fungicide
cellular response to amino acid stimulus
cellular response to manganese ion
cellular response to dsRNA
cellular response to growth factor stimulus
cellular response to lipid
cellular response to organic cyclic compound
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