Invitrogen

NMDAR2B Monoclonal Antibody (NR2B)

NMDAR2B Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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NMDAR2B Antibody (MA1-2014) in IHC (P)

Immunohistochemistry analysis of NMDA Receptor 2B showing staining in the cytoplasm and membrane of paraffin-embedded human breast tissue (right) compared with a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 m... View More

NMDAR2B Antibody in Immunohistochemistry (IHC)

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Product Details

MA1-2014

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

View 24 publications 24 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:100

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1 µg/mL

View 4 publications 4 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

NR2B

Immunogen

Recombinant protein: NR2B subunit of amino acid residues 934-1457.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_560347

Product Specific Information

MA1-2014 detects the N-methyl-D-aspartate (NMDA) receptor type 2B in human and mouse samples.

MA1-2014 has been used successfully in Western blot, IHC (P) and immunocytochemistry procedures. In Western blot analysis of mouse brain tissue this antibody detects a ~166 kDa protein representing NMDA receptor type 2B.

The MA1-2014 immunogen is a recombinant protein composed of amino acid residues 934-1457 of the rat NMDA receptor type 2B.

Target Information

NMDAR2B is a member of the N-methyl-D-aspartate (NMDA) class of ionotropic glutamate receptors. NMDA receptors (NMDAR) have been shown to be involved in long-term potentiation, an activity-dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning. NMDAR play a role in neuronal development and has been implicated in several disorders of the central nervous system including Alzheimer’s Disease, epilepsy and ischemic neuronal cell death. Overexpression of the NR2B subunit of the receptor has been associated with increases in learning and memory while aged, memory impaired animals have deficiencies in NR2B expression. Tyr1472 on NR2B is phosphorylated and this may lead to the increased expression of the NMDAR at the synapse that plays a role in synaptic plasticity. NMDR2B is a heterotrimer comprised of seven enetically encoded and differentially expressed subunits that include NR1, NR2 and NR3. Diseases associated with the dysfunction of NMDAR2B include epileptic encephalopathy, autosomal dominant 6-related mental retardation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Orthogonal approaches required to measure proteasome composition and activity in mammalian brain tissue.

The Journal of biological chemistry

Türker F,Bharadwaj RA,Kleinman JE,Weinberger DR,Hyde TM,White CJ,Williams DW,Margolis SS

Published figure using NMDAR2B monoclonal antibody (Product # MA1-2014) in Western Blot

Thu Jun 01 00:00:00 EDT 2023

First trimester human umbilical cord perivascular cells (HUCPVC) modulate the kynurenine pathway and glutamate neurotransmission in an LPS-induced mouse model of neuroinflammation.

Journal of inflammation (London, England)

Siddiqui F,Gallagher D,Shuster-Hyman H,Lopez L,Gauthier-Fisher A,Librach CL

Published figure using NMDAR2B monoclonal antibody (Product # MA1-2014) in Western Blot

Mon May 01 00:00:00 EDT 2023

Inhibition of LRRK2 Attenuates Depression-Related Symptoms in Mice with Moderate Traumatic Brain Injury.

Cells

Filippone A,Cucinotta L,Bova V,Lanza M,Casili G,Paterniti I,Campolo M,Cuzzocrea S,Esposito E

MA1-2014 was used in Immunohistochemistry to show that moderate traumatic brain injury (mTBI) induction promotes the development of depression-like behavioral changes.

Wed Mar 29 00:00:00 EDT 2023

Inhibition of LRRK2 Attenuates Depression-Related Symptoms in Mice with Moderate Traumatic Brain Injury.

Cells

Filippone A,Cucinotta L,Bova V,Lanza M,Casili G,Paterniti I,Campolo M,Cuzzocrea S,Esposito E

MA1-2014 was used in Immunohistochemistry to show that moderate traumatic brain injury (mTBI) induction promotes the development of depression-like behavioral changes.

Wed Mar 29 00:00:00 EDT 2023

A novel bio-inspired strategy to prevent amyloidogenesis and synaptic damage in Alzheimer's disease.

Molecular psychiatry

Catania M,Colombo L,Sorrentino S,Cagnotto A,Lucchetti J,Barbagallo MC,Vannetiello I,Vecchi ER,Favagrossa M,Costanza M,Giaccone G,Salmona M,Tagliavini F,Di Fede G

MA1-2014 was used in Antibody array to open the way to an unprecedented and promising approach for the prevention of the disease in humans.

Thu Dec 01 00:00:00 EST 2022

Role of Different Doses of Ketamine in Postoperative Neurocognitive Function in Aged Mice Undergoing Partial Hepatectomy by Regulating the Bmal1/NMDA/NF-Κb Axis.

European surgical research. Europaische chirurgische Forschung. Recherches chirurgicales europeennes

Niu X,Zheng S,Li S,Liu H

MA1-2014 was used in Western Blotting to indicate that a subanesthetic dose of ketamine activated Bmal1, downregulated the NMDA/NF-κB axis, and reduced inflammation and microglia activation to alleviate PND in aged mice undergoing PH.

Mon Jan 30 00:00:00 EST 2023

Transient neuroinflammation following surgery contributes to long-lasting cognitive decline in elderly rats via dysfunction of synaptic NMDA receptor.

Journal of neuroinflammation

Chen B,Qin G,Xiao J,Deng X,Lin A,Liu H

MA1-2014 was used in Western Blotting to identify sustained synaptic NR2A and NR2B downregulation and hypofunction induced by transient neuroinflammation following surgery as important contributors to the development of Perioperative neurocognitive disorders in elderly rats.

Wed Jul 13 00:00:00 EDT 2022

Profiling prefrontal cortex protein expression in rats exhibiting an incubation of cocaine craving following short-access self-administration procedures.

Frontiers in psychiatry

Huerta Sanchez LL,Sankaran M,Li TL,Doan H,Chiu A,Shulman E,Shab G,Kippin TE,Szumlinski KK

MA1-2014 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to highlight Akt1-related signaling within the prelimbic cortex driving incubated cocaine craving, reveal a role for CaMKII-dependent signaling through glutamate receptors in this behavioral phenomenon.

Tue Jan 24 00:00:00 EST 2023

TRPV4 Regulates Soman-Induced Status Epilepticus and Secondary Brain Injury via NMDA Receptor and NLRP3 Inflammasome.

Neuroscience bulletin

Wang S,He H,Long J,Sui X,Yang J,Lin G,Wang Q,Wang Y,Luo Y

MA1-2014 was used in Western Blotting to evaluate the potential effects of transient receptor vanilloid 4 (TRPV4) in the treatment of soman-induced status epilepticus (SE) and secondary brain injury.

Thu Jul 01 00:00:00 EDT 2021

The σ1 Receptor and the HINT1 Protein Control α2δ1 Binding to Glutamate NMDA Receptors: Implications in Neuropathic Pain.

Biomolecules

Rodríguez-Muñoz M,Cortés-Montero E,Onetti Y,Sánchez-Blázquez P,Garzón-Niño J

Published figure using NMDAR2B monoclonal antibody (Product # MA1-2014) in Western Blot

Fri Nov 12 00:00:00 EST 2021

Neurobiology of aging

Evans AK,Park HH,Saw NL,Singhal K,Ogawa G,Leib RD,Shamloo M

MA1-2014 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to examine effects of age and acute beta-blocker administration on LC and hippocampus pathology, neuroinflammation and learning and memory behavior in mice.

Fri Oct 01 00:00:00 EDT 2021

Tau knockout exacerbates degeneration of parvalbumin-positive neurons in substantia nigra pars reticulata in Parkinson's disease-related α-synuclein A53T mice.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Jiao L,Zheng M,Duan J,Wu T,Li Z,Liu L,Xiang X,Tang X,He J,Li X,Zhang G,Ding J,Cai H,Lin X

MA1-2014 was used in Immunohistochemistry (Frozen) to investigate how the expression of tau affects α-syn-induced neurodegeneration in vivo (murine model), a key step in the pathogenesis of Parkinson's disease.

Tue Sep 01 00:00:00 EDT 2020

DAPK1 Promotes Extrasynaptic GluN2B Phosphorylation and Striatal Spine Instability in the YAC128 Mouse Model of Huntington Disease.

Frontiers in cellular neuroscience

Schmidt ME,Caron NS,Aly AE,Lemarié FL,Dal Cengio L,Ko Y,Lazic N,Anderson L,Nguyen B,Raymond LA,Hayden MR

Published figure using NMDAR2B monoclonal antibody (Product # MA1-2014) in Western Blot

Tue Dec 01 00:00:00 EST 2020

AMPA Receptor Dysregulation and Therapeutic Interventions in a Mouse Model of CDKL5 Deficiency Disorder.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Yennawar M,White RS,Jensen FE

MA1-2014 was used in Western Blotting to investigate changes in synaptic glutamate receptor subunits and functional consequences.

Wed Jun 12 00:00:00 EDT 2019

Altered Regulation of Striatal Neuronal N-Methyl-D-Aspartate Receptor Trafficking by Palmitoylation in Huntington Disease Mouse Model.

Frontiers in synaptic neuroscience

Kang R,Wang L,Sanders SS,Zuo K,Hayden MR,Raymond LA

MA1-2014 was used in Western Blotting to study the mechanism of altered GluN2B trafficking in Huntington disease.

Tue Sep 29 00:00:00 EDT 2020

Chronic Toxoplasma gondii Infection Induces Anti-N-Methyl-d-Aspartate Receptor Autoantibodies and Associated Behavioral Changes and Neuropathology.

Infection and immunity

Li Y,Viscidi RP,Kannan G,McFarland R,Pletnikov MV,Severance EG,Yolken RH,Xiao J

MA1-2014 was used in Western Blotting to study whether parasite infection is the cause of NMDAR autoantibodies.

Mon Oct 01 00:00:00 EDT 2018

Disruption of Ninjurin1 Leads to Repetitive and Anxiety-Like Behaviors in Mice.

Molecular neurobiology

Le H,Ahn BJ,Lee HS,Shin A,Chae S,Lee SY,Shin MW,Lee EJ,Cha JH,Son T,Seo JH,Wee HJ,Lee HJ,Jang Y,Lo EH,Jeon S,Oh GT,Kim D,Kim KW

MA12014 was used in western blot to report the abnormal behavioral phenotypes of mice deficient in Ninjurin 1

Wed Nov 01 00:00:00 EDT 2017

Overexpression of human NR2B receptor subunit in LMAN causes stuttering and song sequence changes in adult zebra finches.

Scientific reports

Chakraborty M,Chen LF,Fridel EE,Klein ME,Senft RA,Sarkar A,Jarvis ED

MA12014 was used in immunocytochemistry and western blot to propose that N-methyl-D-aspartate subtype 2B glutamate receptor subunit expression in the adult lateral magnocellular nucleus of the anterior nidopallium is important adult courtship song

Fri Apr 21 00:00:00 EDT 2017

Overexpression of human NR2B receptor subunit in LMAN causes stuttering and song sequence changes in adult zebra finches.

Scientific reports

Chakraborty M,Chen LF,Fridel EE,Klein ME,Senft RA,Sarkar A,Jarvis ED

Fri Apr 21 00:00:00 EDT 2017

Neurodegeneration in Amygdala Precedes Hippocampus in the APPswe/ PS1dE9 Mouse Model of Alzheimer's Disease.

Current Alzheimer research

Lin TW,Liu YF,Shih YH,Chen SJ,Huang TY,Chang CY,Lien CH,Yu L,Chen SH,Kuo YM

MA1-2014 was used in western blot to elucidate the APPswe/PS1dE9 mouse model of Alzheimer's disease that shows neurodegeneration in amygdala precedes hippocampus

Fri Aug 26 00:00:00 EDT 2016

Environmental enrichment mitigates the sex-specific effects of gestational inflammation on social engagement and the hypothalamic pituitary adrenal axis-feedback system.

Brain, behavior, and immunity

Connors EJ,Shaik AN,Migliore MM,Kentner AC

MA1-2014 was used in western blot to study the effect of environment enrichment on maternal immune activation

Sat Nov 01 00:00:00 EDT 2014

Disulfide-containing high mobility group box-1 promotes N-methyl-D-aspartate receptor function and excitotoxicity by activating Toll-like receptor 4-dependent signaling in hippocampal neurons.

Antioxidants & redox signaling

Balosso S,Liu J,Bianchi ME,Vezzani A

MA1-2014 was used in western blot to study the role of hippocampal neuronal TLR4 signaling activation in the mechanism by which HMGB1 drives NMDAR hyperexcitability

Mon Oct 20 00:00:00 EDT 2014

The influence of neuropathology on brain inflammation in human and experimental temporal lobe epilepsy.

Journal of neuroimmunology

Aalbers MW,Rijkers K,Majoie HJ,Dings JT,Schijns OE,Schipper S,De Baets MH,Kessels A,Vles JS,Hoogland G

MA1-2014 was used in western blot to study whether neuropathological changes contribute to temporal lobe epilepsy brain inflammation

Sun Jun 15 00:00:00 EDT 2014

Mitigation of augmented extrasynaptic NMDAR signaling and apoptosis in cortico-striatal co-cultures from Huntington's disease mice.

Neurobiology of disease

Milnerwood AJ,Kaufman AM,Sepers MD,Gladding CM,Zhang L,Wang L,Fan J,Coquinco A,Qiao JY,Lee H,Wang YT,Cynader M,Raymond LA

MA1-2014 was used in western blot to study changes in NMDAR signaling and apoptosis in cortico-striatal co-cultures from Huntington's disease mice

Mon Oct 01 00:00:00 EDT 2012

N-methyl-D-aspartate receptor subunit- and neuronal-type dependence of excitotoxic signaling through post-synaptic density 95.

Journal of neurochemistry

Fan J,Vasuta OC,Zhang LY,Wang L,George A,Raymond LA

Published figure using NMDAR2B monoclonal antibody (Product # MA1-2014) in Western Blot

Mon Nov 01 00:00:00 EDT 2010

Dopamine D(1) receptor-mediated enhancement of NMDA receptor trafficking requires rapid PKC-dependent synaptic insertion in the prefrontal neurons.

Journal of neurochemistry

Li YC,Liu G,Hu JL,Gao WJ,Huang YQ

MA1-2014 was used in Immunocytochemistry to suggest that D(1)-mediated enhancement of NMDA current depends on the NMDA receptor trafficking through rapid synaptic insertion and both PKA and PKC signaling pathways play important roles in the regulatory process.

Thu Jul 01 00:00:00 EDT 2010

TRPV1 acts as a synaptic protein and regulates vesicle recycling.

Journal of cell science

Goswami C,Rademacher N,Smalla KH,Kalscheuer V,Ropers HH,Gundelfinger ED,Hucho T

MA1-2014 was used in immunocytochemistry to study the role TRPV1 as a synaptic protein regulating vesicle recycling

Tue Jun 15 00:00:00 EDT 2010

Age and meloxicam attenuate the ischemia/reperfusion-induced down-regulation in the NMDA receptor genes.

Neurochemistry international

Montori S,Dos-Anjos S,Martínez-Villayandre B,Regueiro-Purriños MM,Gonzalo-Orden JM,Ruano D,Fernández-López A

MA1-2014 was used in western blot to study NMDA receptor gene responses to ischemia

Thu Jul 01 00:00:00 EDT 2010

Neuronal survival and resistance to HIV-1 Tat toxicity in the primary culture of rat fetal neurons.

Experimental neurology

Aksenova MV,Aksenov MY,Adams SM,Mactutus CF,Booze RM

MA1-2014 was used in Immunocytochemistry to demonstrate that rapid binding/uptake of Tat 1-86 for 2 h was sufficient to trigger caspase activation and neurodegeneration in rat fetal midbrain cell cultures.

Sun Feb 01 00:00:00 EST 2009

Nonspecific interaction of prefibrillar amyloid aggregates with glutamatergic receptors results in Ca2+ increase in primary neuronal cells.

The Journal of biological chemistry

Pellistri F,Bucciantini M,Relini A,Nosi D,Gliozzi A,Robello M,Stefani M

MA1-2014 was used in immunocytochemistry to study the Ca(2+) increase in neuronal cells induced by the non-specific interaction of pre-fibrillar amyloid aggregates with glutaminergic receptors

Fri Oct 31 00:00:00 EDT 2008

Cell-specific expression of N-methyl-D-aspartate receptor subunits in Müller glia and neurons from the chick retina.

Investigative ophthalmology & visual science

Lamas M,Lee-Rivera I,López-Colomé AM

MA1-2014 was used in Western Blotting to analyse the structural differences in N-methyl-D-aspartate receptors and assess their role in the differential participation of neurons and glia in the physiologic control of glutamate transmission in the retina.

Sat Oct 01 00:00:00 EDT 2005

Characterization of NMDA receptor subunit-specific antibodies: distribution of NR2A and NR2B receptor subunits in rat brain and ontogenic profile in the cerebellum.

Journal of neurochemistry

Wang YH,Bosy TZ,Yasuda RP,Grayson DR,Vicini S,Pizzorusso T,Wolfe BB

MA1-2014 was used in western blot to investigate the localization of NMDA receptor subunits in the rat brain and the corresponding expression changes during development using novel subunit-specific antibodies

Sat Jul 01 00:00:00 EDT 1995

Bioinformatics

Protein Aliases: GluN2B; GluRepsilon2; Glutamate [NMDA] receptor subunit epsilon-2; glutamate receptor; Glutamate receptor ionotropic, NMDA 2B; glutamate receptor subunit epsilon-2; glutamate receptor, ionotropic, N-methyl D-aspartate 2B; glutamate receptor, ionotropic, NMDA2B; GRIN 2B; HGNC:4586; hNR 3; MGC142178; MGC142180; N-methyl D-aspartate receptor 2B; N-methyl D-aspartate receptor subtype 2B; N-methyl-D-aspartate receptor subunit 3; NMDAR2B; NMDE2; NME2; NR2B; NR3

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Gene Aliases: AW490526; EIEE27; GluN2B; GRIN2B; hNR3; MRD6; NMDAR2B; NR2B

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UniProt ID: (Human) Q13224, (Mouse) Q01097, (Rat) Q00960

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Entrez Gene ID: (Human) 2904, (Mouse) 14812, (Rat) 24410

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Function(s)

NMDA glutamate receptor activity Ras guanyl-nucleotide exchange factor activity extracellular-glutamate-gated ion channel activity protein binding zinc ion binding glycine binding receptor activity ionotropic glutamate receptor activity receptor binding interleukin-1 receptor binding ion channel activity cation channel activity calcium channel activity beta-catenin binding drug binding D2 dopamine receptor binding ionotropic glutamate receptor binding neurotransmitter binding metal ion binding protein heterodimerization activity cell adhesion molecule binding transmembrane signal receptor

Process(es)

MAPK cascade transport glutamate receptor signaling pathway chemical synaptic transmission learning or memory ion transmembrane transport ionotropic glutamate receptor signaling pathway positive regulation of GTPase activity response to ethanol ephrin receptor signaling pathway action potential behavioral fear response in utero embryonic development startle response suckling behavior ion transport cation transport calcium ion transport sensory organ development learning memory associative learning embryo development regulation of protein kinase A signaling positive regulation of cell death positive regulation of glutamate secretion synaptic transmission, glutamatergic fear response receptor clustering regulation of MAPK cascade regulation of synaptic plasticity regulation of neuronal synaptic plasticity regulation of long-term neuronal synaptic plasticity behavioral response to pain rhythmic process positive regulation of synaptic transmission detection of mechanical stimulus involved in sensory perception of pain regulation of postsynaptic membrane potential excitatory postsynaptic potential long-term synaptic potentiation calcium ion transmembrane transport cation transmembrane transport response to amphetamine long-term memory response to mechanical stimulus response to toxic substance response to carbohydrate response to manganese ion cellular response to magnesium starvation response to organic cyclic compound response to amine hippocampus development cerebral cortex development response to magnesium ion multicellular organismal response to stress response to cytokine response to cocaine response to calcium ion response to methylmercury response to electrical stimulus response to other organism response to growth hormone response to fungicide cellular response to amino acid stimulus cellular response to manganese ion cellular response to dsRNA cellular response to growth factor stimulus cellular response to lipid cellular response to organic cyclic compound

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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NMDAR2B Monoclonal Antibody (NR2B)

NMDAR2B Antibody (MA1-2014) in IHC (P)

Product Details

MA1-2014

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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