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NNT Antibody (PA5-143849) in ICC/IF
Immunocytochemistry analysis of NNT in U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in NNT Polyclonal antibody (Product # PA5-143849) using a dilution of 4 μg/mL. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for t... View More
Product Details
PA5-143849
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Rat,
Human,
Mouse,
Non-human primate
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human NNT recombinant protein (Position: C44-A350).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.01mg sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_3075063
Product Specific Information
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Target Information
This gene encodes an integral protein of the inner mitochondrial membrane. The enzyme couples hydride transfer between NAD(H) and NADP(+) to proton translocation across the inner mitochondrial membrane. Under most physiological conditions, the enzyme uses energy from the mitochondrial proton gradient to produce high concentrations of NADPH. The resulting NADPH is used for biosynthesis and in free radical detoxification.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: NAD(P) transhydrogenase, mitochondrial; Nicotinamide nucleotide transhydrogenase; Nicotinamide nucleotide transhydrogenase (NAD(P)+ transhydrogenase); Pyridine nucleotide transhydrogenase
Gene Aliases: 4930423F13Rik; AI323702; BB168308; GCCD4; NNT
UniProt ID: (Human) Q13423
Entrez Gene ID: (Human) 23530, (Rat) 310378, (Mouse) 18115
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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