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Product Details
H00004843-M11
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
2G4
Immunogen
NOS2 (NP_000616, 685 a.a. approximately 794 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Conjugate
Form
Liquid
Concentration
See Label
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
no preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Sequence of this protein is as follows: DVRGKQHIQI PKLYTSNVTW DPHHYRLVQD SQPLDLSKAL SSMHAKNVFT MRLKSRQNLQ SPTSSRATIL VELSCEDGQG LNYLPGEHLG VCPGNQPALV QGILERVVDG
Target Information
iNOS (Inducible Nitric oxide, NO, NOS) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. iNOS is expressed in liver and inducible by a combination of lipopolysaccharide and certain cytokines. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) and endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains. iNOS is found in a variety of cell types including macrophages, hepatocytes, synoviocytes, and smooth muscle cells. Cytokines such as interferon-gamma (IFN), tumor necrosis factor (TNF), interleukin-1 and -2, and lipopolysaccarides (LPS) cause an increase in iNOS mRNA, protein, and activity levels. Protein kinase C-stimulating agents exhibit the same effect on iNOS activity. After cytokine induction, iNOS exhibits a delayed activity response which is then followed by a significant increase in NO production over a long period of time. Three related iNOS pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17. Diseases associated with iNOS dysfunction include achalasia and impotence.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: HEP-NOS; Hepatocyte NOS; hepatocytes; Inducible NO synthase; Inducible NOS; nitric oxide synthase 2, inducible; nitric oxide synthase 2A (inducible, hepatocytes); Nitric oxide synthase, inducible; nitric oxide synthase, macrophage; nitric oxide synthase-inducible; NOS type II; NOS, type II; OTTMUSP00000000202; Peptidyl-cysteine S-nitrosylase NOS2
Gene Aliases: HEP-NOS; INOS; NOS; NOS2; NOS2A
UniProt ID: (Human) O94994
Entrez Gene ID: (Human) 4843
response to hypoxia
positive regulation of leukocyte mediated cytotoxicity
innate immune response in mucosa
arginine catabolic process
superoxide metabolic process
nitric oxide biosynthetic process
nitric oxide mediated signal transduction
circadian rhythm
response to bacterium
negative regulation of gene expression
peptidyl-cysteine S-nitrosylation
positive regulation of guanylate cyclase activity
prostaglandin secretion
cellular response to drug
regulation of cell proliferation
negative regulation of protein catabolic process
defense response to bacterium
regulation of cellular respiration
cell redox homeostasis
negative regulation of blood pressure
positive regulation of vasodilation
regulation of insulin secretion
defense response to Gram-negative bacterium
positive regulation of killing of cells of other organism
oxidation-reduction process
cellular response to lipopolysaccharide
cellular response to interferon-gamma
interleukin-6 secretion
interleukin-8 secretion
regulation of cytokine production involved in inflammatory response
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