Proteintech

NOS3 Polyclonal Antibody

NOS3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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NOS3 Antibody (27120-1-AP) in IHC (P)

Immunohistochemical analysis of paraffin-embedded human tonsilitis tissue slide using 27120-1-AP (NOS3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Product Details

27120-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500

View 19 publications 19 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Mouse, Rat

Published species

Not Applicable

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

NOS3 Fusion Protein Ag25712

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

700 µg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

Product Specific Information

Aliquoting is unnecessary for -20°C storage.

Target Information

Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) and endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Exosomes Derived from AT2R-Overexpressing BMSC Prevent Restenosis After Carotid Artery Injury by Attenuating the Injury-Induced Neointimal Hyperplasia.

Journal of cardiovascular translational research

Zou X,Liao Y,Liu Z,Xu X,Sun W,Qin H,Wang H,Liu J,Jing T

Wed Feb 01 00:00:00 EST 2023

Exosomes Derived from AT2R-Overexpressing BMSC Prevent Restenosis After Carotid Artery Injury by Attenuating the Injury-Induced Neointimal Hyperplasia.

Journal of cardiovascular translational research

Zou X,Liao Y,Liu Z,Xu X,Sun W,Qin H,Wang H,Liu J,Jing T

Wed Feb 01 00:00:00 EST 2023

Fucoidan promotes angiogenesis and accelerates wound healing through AKT/Nrf2/HIF-1α signalling pathway.

International wound journal

Wen W,Yang L,Wang X,Zhang H,Wu F,Xu K,Chen S,Liao Z

Wed Nov 01 00:00:00 EDT 2023

Exercise protects aged mice against coronary endothelial senescence via FUNDC1-dependent mitophagy.

Redox biology

Ma L,Li K,Wei W,Zhou J,Li Z,Zhang T,Wangsun Y,Tian F,Dong Q,Zhang H,Xing W

Thu Jun 01 00:00:00 EDT 2023

Dahuang Zhechong pill attenuates hepatic sinusoidal capillarization in liver cirrhosis and hepatocellular carcinoma rat model via the MK/integrin signaling pathway.

Journal of ethnopharmacology

Fu C,Zhang Y,Xi WJ,Xu K,Meng F,Ma T,Li W,Wu L,Chen Z

Tue May 23 00:00:00 EDT 2023

Extracellular vesicles from human urine-derived stem cells merged in hyaluronic acid ameliorate erectile dysfunction in type 2 diabetic rats by glans administration.

Andrology

Zhuang J,Gao P,Chen H,Fang Z,Zheng J,Zhu D,Hou J

Tue Nov 01 00:00:00 EDT 2022

Substrate stiffness regulates differentiation of induced pluripotent stem cells into heart valve endothelial cells.

Acta biomaterialia

Cao H,Zhou Q,Liu C,Zhang Y,Xie M,Qiao W,Dong N

Fri Apr 15 00:00:00 EDT 2022

Naoluo Xintong Decoction in the Treatment of Ischemic Stroke: A Network Analysis of the Mechanism of Action.

Frontiers in pharmacology

Wang N,Chu F,Fei C,Pan L,Wang Y,Chen W,Peng D,Duan X,He L

Sat Jul 16 00:00:00 EDT 2022

Pretreatment with Shenmai Injection Protects against Coronary Microvascular Dysfunction.

Evidence-based complementary and alternative medicine : eCAM

Zheng Z,Yu Z,Xu B,Zhou Y,Xing Y,Li Q,Tang W,Peng F

Sat Jul 16 00:00:00 EDT 2022

Existence of multiple organ aging in animal model of emphysema induced by cigarette smoke extract.

Tobacco induced diseases

Liang G,He Z,Chen Y,Zhang H,Peng H,Zong D,Long Y

Sat Jan 29 00:00:00 EST 2022

Oridonin attenuates low shear stress-induced endothelial cell dysfunction and oxidative stress by activating the nuclear factor erythroid 2-related factor 2 pathway.

BMC complementary medicine and therapies

Chen Z,Liu H,Zhao X,Mamateli S,Liu C,Wang L,Yu J,Liu Y,Cai J,Qiao T

Thu Jul 07 00:00:00 EDT 2022

A transcriptomics and molecular biology based investigation reveals the protective effect and mechanism of carnosol on t-BHP induced HRMECs via Nrf2 signaling pathway.

European journal of pharmacology

Ren Y,Liu Y,Liu K,Shu Z,Lv T,Chen Z,Feng W,Zhang Y

Sun May 15 00:00:00 EDT 2022

Macrophage-derived exosomal miR-4532 promotes endothelial cells injury by targeting SP1 and NF-κB P65 signalling activation.

Journal of cellular and molecular medicine

Liu P,Wang S,Wang G,Zhao M,Du F,Li K,Wang L,Wu H,Chen J,Yang Y,Su G

Sat Oct 01 00:00:00 EDT 2022

Cilostazol regulates the expressions of endothelin-1 and endothelial nitric oxide synthase via activation of the p38 MAPK signaling pathway in HUVECs.

Biomedical reports

Xue Y,Li X,Wang Z,Lv Q

Thu Sep 01 00:00:00 EDT 2022

Hax-1 Regulates Radiation-Induced Mitochondrial-Dependent Apoptosis of Uveal Melanoma Cells through PI3K/AKT/eNOS Pathway.

Journal of oncology

Wang S,Tan J,Chen L,Wang J

Sat Jul 16 00:00:00 EDT 2022

Tissue Kallikrein Protects Rat Prostate against the Inflammatory Damage in a Chronic Autoimmune Prostatitis Model via Restoring Endothelial Function in a Bradykinin Receptor B2-Dependent Way.

Oxidative medicine and cellular longevity

Zhang M,Lin D,Luo C,Wei P,Cui K,Chen Z

Fri Mar 18 00:00:00 EDT 2022

Decreased nitric oxide content mediated by asymmetrical dimethylarginine and protein l-arginine methyltransferase 3 in macrophages induces trophoblast apoptosis: a potential cause of recurrent miscarriage.

Human reproduction (Oxford, England)

Hao F,Tang LC,Sun JX,Li WX,Zhao Y,Xu XH,Jin LP

Thu Nov 18 00:00:00 EST 2021

Clopidogrel-Induced Gastric Injury in Rats is Attenuated by Stable Gastric Pentadecapeptide BPC 157.

Drug design, development and therapy

Wu H,Wei M,Li N,Lu Q,Shrestha SM,Tan J,Zhang Z,Wu G,Shi R

Tue Sep 21 00:00:00 EDT 2021

Antihypertensive effects of Trichiurus lepturus myosin hydrolysate in spontaneously hypertensive rats.

Food & function

Fu W,Wang P,Wu H,Zhang Z,Zeng H,Zhang Y,Zheng B,Hu J

Thu Apr 30 00:00:00 EDT 2020

MiR-30a-5p ameliorates spinal cord injury-induced inflammatory responses and oxidative stress by targeting Neurod 1 through MAPK/ERK signalling.

Clinical and experimental pharmacology & physiology

Fu X,Shen Y,Wang W,Li X

Mon Jan 01 00:00:00 EST 2018

Procyanidins extracted from the litchi pericarp ameliorate atherosclerosis in ApoE knockout mice: their effects on nitric oxide bioavailability and oxidative stress.

Food & function

Rong S,Hu X,Zhao S,Zhao Y,Xiao X,Bao W,Liu L

Wed Nov 15 00:00:00 EST 2017

Procyanidins extracted from the litchi pericarp ameliorate atherosclerosis in ApoE knockout mice: their effects on nitric oxide bioavailability and oxidative stress.

Food & function

Rong S,Hu X,Zhao S,Zhao Y,Xiao X,Bao W,Liu L

Wed Nov 15 00:00:00 EST 2017

Pitavastatin up-regulates eNOS production by suppressing miR-155 expression in lipopolysaccharide-stimulated human umbilical vein endothelial cells.

Cardiovascular therapeutics

Jing C,Guo M,Bao X,Li T,Lin J,Lu X,Wang W

Sun Oct 01 00:00:00 EDT 2017

Bioinformatics

Protein Aliases: cNOS; Constitutive NOS; e-NOS; EC-NOS; endothelial nitric oxide synthase; endothelial nitric oxide synthase 3; Endothelial NOS; Nitric oxide synthase 3; Nitric oxide synthase, endothelial; NOS type III; NOSIII; OTTHUMP00000213183

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Gene Aliases: 2310065A03Rik; Ecnos; eNOS; Nos-3; Nos3

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UniProt ID: (Mouse) P70313, (Rat) Q62600

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Entrez Gene ID: (Mouse) 18127, (Rat) 24600

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Function(s)

actin binding actin monomer binding NADPH-hemoprotein reductase activity nitric-oxide synthase activity iron ion binding calmodulin binding beta-catenin binding FMN binding oxidoreductase activity heme binding tetrahydrobiopterin binding arginine binding cadherin binding metal ion binding flavin adenine dinucleotide binding NADP binding nitric-oxide synthase binding Hsp90 protein binding scaffold protein binding protein binding oxidoreductase protein modifying enzyme

Process(es)

angiogenesis ovulation from ovarian follicle in utero embryonic development blood vessel remodeling regulation of sodium ion transport regulation of the force of heart contraction by chemical signal regulation of systemic arterial blood pressure by endothelin arginine catabolic process nitric oxide biosynthetic process signal transduction nitric oxide mediated signal transduction negative regulation of cell proliferation negative regulation of muscle hyperplasia smooth muscle hyperplasia lung development positive regulation of guanylate cyclase activity lipopolysaccharide-mediated signaling pathway response to estradiol positive regulation of apoptotic process negative regulation of potassium ion transport endothelial cell migration positive regulation of angiogenesis negative regulation of blood pressure positive regulation of vasodilation negative regulation of smooth muscle cell proliferation regulation of blood vessel size negative regulation of hydrolase activity negative regulation of calcium ion transport oxidation-reduction process cellular response to mechanical stimulus cellular response to transforming growth factor beta stimulus response to hypoxia female pregnancy aging response to mechanical stimulus response to fructose response to metal ion response to lead ion response to organic cyclic compound response to activity response to lipopolysaccharide response to cytokine response to fluid shear stress cellular response to heat response to statin response to drug response to amino acid response to peptide hormone response to ethanol response to axon injury response to corticosterone response to hyperoxia response to lipoprotein particle retina development in camera-type eye bone development cellular response to magnesium ion cellular response to cAMP cellular response to tumor necrosis factor cellular response to growth factor stimulus cellular response to organic cyclic compound response to candesartan response to peptide response to ketone response to salt

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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NOS3 Polyclonal Antibody

NOS3 Antibody (27120-1-AP) in IHC (P)

Product Details

27120-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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