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Product Details
H00004846-M04
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2a, kappa
Class
Monoclonal
Type
Antibody
Clone
1D12
Immunogen
NOS3 (AAH63294.1, 61 a.a. approximately 160 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Conjugate
Form
Liquid
Concentration
See Label
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
no preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Sequence of this protein is as follows: QPPEGPKFPR VKNWEVGSIT YDTLSAQAQQ DGPCTPRRCL GSLVFPRKLQ GRPSPGPPAP EQLLSQARDF INQYYSSIKR SGSQAHEQRL QEVEAEVAAT
Target Information
Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) and endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: cNOS; Constitutive NOS; e-NOS; EC-NOS; Endothelial NOS; Nitric oxide synthase 3; nitric oxide synthase 3 (endothelial cell); nitric oxide synthase 3 transcript variant eNOS-delta20; nitric oxide synthase 3 transcript variant eNOS-delta20-21; nitric oxide synthase 3 transcript variant eNOS-delta21; Nitric oxide synthase, endothelial; NOS type III; NOSIII; OTTHUMP00000213183
Gene Aliases: ECNOS; eNOS; NOS3
UniProt ID: (Human) P29474
Entrez Gene ID: (Human) 4846
actin monomer binding
nitric-oxide synthase activity
iron ion binding
protein binding
calmodulin binding
FMN binding
heme binding
tetrahydrobiopterin binding
arginine binding
cadmium ion binding
flavin adenine dinucleotide binding
NADP binding
scaffold protein binding
oxidoreductase
protein modifying enzyme
angiogenesis
ovulation from ovarian follicle
in utero embryonic development
blood vessel remodeling
regulation of sodium ion transport
regulation of the force of heart contraction by chemical signal
regulation of systemic arterial blood pressure by endothelin
arginine catabolic process
nitric oxide biosynthetic process
mitochondrion organization
nitric oxide mediated signal transduction
regulation of blood pressure
negative regulation of cell proliferation
response to heat
negative regulation of platelet activation
negative regulation of muscle hyperplasia
smooth muscle hyperplasia
removal of superoxide radicals
lung development
positive regulation of guanylate cyclase activity
lipopolysaccharide-mediated signaling pathway
response to fluid shear stress
negative regulation of potassium ion transport
endothelial cell migration
cell redox homeostasis
positive regulation of angiogenesis
negative regulation of blood pressure
positive regulation of vasodilation
regulation of blood vessel size
regulation of nitric-oxide synthase activity
negative regulation of hydrolase activity
negative regulation of calcium ion transport
oxidation-reduction process
negative regulation of extrinsic apoptotic signaling pathway via death domain receptors
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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